PCR detection of alternaria spp. in processed foods, based on the internal transcribed spacer genetic marker

Journal of Food Protection

Volumn 74, Issue 2, 2011, Pages 240-247

  Pavón, Miguel Ángel ^a   González, Isabel ^a   Rojas, María ^a   Pegels, Nicolette ^a   Martín, Rosario ^a   García, Teresa ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA; PRIMER DNA; SPACER DNA;

ALTERNARIA; ARTICLE; FOOD CONTAMINATION; GENETIC MARKER; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRODUCT SAFETY; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; TOMATO;

ALTERNARIA; BASE SEQUENCE; CONSUMER PRODUCT SAFETY; DNA PRIMERS; DNA, FUNGAL; DNA, INTERGENIC; FOOD CONTAMINATION; GENETIC MARKERS; HUMANS; LYCOPERSICON ESCULENTUM; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY;

ALTERNARIA; ANIMALIA; BACTERIA (MICROORGANISMS); LYCOPERSICON ESCULENTUM;

EID: 79951589524     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X.JFP-10-110     Document Type: Article

References (39)

1. Andersen, B., A. Dongo, and B. M. Pryor. 2008. Secondary metabolite profiling of Alternaria dauci, A. porri, A. solani, and A. tomatophila. Mycol. Res. 112:241-250.
2. Andersen, B., and J. Frisvad. 2004. Natural occurrence of fungi and fungal metabolites in moldy tomatoes. J. Agric. Food Chem. 52:7507-7513.
3. Andersen, B., E. Kroger, and R. G. Roberts. 2001. Chemical and morphological segregation of Alternaria alternata, A. gaisen, and A. longipes. Mycol. Res. 105:291-299. (Pubitemid 32258461)
4. Andersen, B., E. Kroger, and R. G. Roberts. 2002. Chemical and morphological segregation of Alternaria arborescens, A. infectoria, and A. tenuissima species groups. Mycol. Res. 106:170-182. (Pubitemid 34514184)
5. Andersen, B., J. L. Sorensen, K. F. Nielsen, B. G. van den Ende, and S. de Hoog. 2009. A polyphasic approach to the taxonomy of the Alternaria infectoria species group. Fungal Genet. Biol. 46:642-656.
6. Azcarate, M. P., A. Patriarca, L. Terminiello, and P. Fernandez. 2008. Alternaria toxins in wheat during the 2004 to 2005 Argentinean harvest. J. Food Prot. 71:1262-1265. (Pubitemid 351829826)
7. Bottalico, A., and A. Logrieco. 1998. Toxigenic Alternaria species of economic importance, p. 65-108. In K. K. Sinha and D. Bhatnager (ed.), Mycotoxins in agriculture and food safety. Marcel Dekker, New York.
8. Chou, H., and W. Wu. 2002. Phylogenetic analysis of internal transcribed spacer regions of the genus Alternaria, and the significance of filament-beaked conidia. Mycol. Res. 106:164-169. (Pubitemid 34514183)
9. Christensen, K. B., J. W. van Klink, R. T. Weavers, T. O. Larsen, B. Andersen, and R. K. Phipps. 2005. Novel chemotaxonomic markers for the Alternaria infectoria species group. J. Agric. Food Chem. 53:431-435.
10. Dugan, F. M., and T. L. Peever. 2002. Morphological and cultural differentiation of described species of Alternaria from Poaceae.Mycotaxon 83:229-264. (Pubitemid 35456744)
11. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791.
12. Iniesta, M. D., D. Pérez-Conesa, J. Garcýa-Alonso, G. Ros, and M. J. Periago. 2009. Folate content in tomato (Lycopersicon esculentum). Influence of cultivar, ripeness, year of harvest, and pasteurization and storage temperatures. J. Agric. Food Chem. 57:4739-4745.
13. Konstantinova, P., P. Bonants, M. van Gent-Pelzer, P. van der Zouwen, and R. van den Bula. 2002. Development of specific primers for detection and identification of Alternaria spp. in carrot material by PCR and comparison with blotter and plating assays. Mycol. Res.106:23-33. (Pubitemid 34186311)
14. Kusaba, M., and T. Tsuge. 1995. Phylogeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA. Curr. Genet. 28:491-498.
15. Liu, G. T., Y. Z. Qian, P. Zhang, Z. M. Dong, Y. M. Qi, and H. T. Guo. 1992. Etiological role of Alternaria alternata in human esophageal cancer. Chin. Med. J. 105:390-400.
16. Logrieco, A., A. Bottalico, M. Mulé, A. Moretti, and G. Perrone. 2003. Epidemiology of toxigenic fungi and their associated mycotoxins for some Mediterranean crops. Eur. J. Plant Pathol.109:645-667. (Pubitemid 37325506)
17. Marek, P., T. Annamalai, and K. Venkitanarayan. 2003. Detection of Penicillium expansum by polymerase chain reaction. Int. J. Food Microbiol. 89:139-144. (Pubitemid 37412687)
18. Motta, S., and L. M. Valente Soares. 2000. Simultaneous determination of tenuazonic and cyclopiazonic acids in tomato products. Food Chem. 71:111-116. (Pubitemid 30621547)
19. Murphy, P. A., S. Hendrich, C. Landgren, and C. M. Bryant. 2006. Food mycotoxins: an update. J. Food Sci. 71:51-65.
20. National Center for Biotechnology Information. 2009. Basic Local Alignment Search Tool. Available at: http://blast.ncbi.nlm.nih.gov/Blast.cgi. Accessed 1 March 2010.
21. Nei, M., and S. Kumar. 2000. Molecular evolution and phylogenetics. Oxford University Press, New York.
22. Niessen, L. 2007. PCR-based diagnosis and quantification of mycotoxin producing fungi. Int. J. Food Microbiol. 119:38-46. (Pubitemid 350051464)
23. Ostry, V. 2008. Alternaria mycotoxins: an overview of chemical characterization, producers, toxicity, analysis and occurrence in foodstuffs. World Mycotoxin J. 1:175-188.
24. Peever, T. L., G. Su, L. Carpenter-Boggs, and L. W. Timmer. 2004. Molecular systematics of citrus-associated Alternaria species.Mycologia 96:119-134. (Pubitemid 38300162)
25. Perelló , A. E., M. Moreno, and M. N. Sisterna, 2008. Alternaria infectoria species group associated with black point of wheat in Argentina. Plant Pathol. 57:379. (Pubitemid 351422449)
26. Perelló , A. E., and M. N. Sisterna. 2006. Leaf blight of wheat caused by Alternaria triticina in Argentina. Plant Pathol. 55:303. (Pubitemid 351209335)
27. Pitt, J. I., and A. D. Hocking. 1997. Fungi and food spoilage. Blackie Academic and Professional, London.
28. Pozzi, C. R., R. Braghini, J. Arcaro, P. Zorzete, A. L. Israel, I. O. Pozar, S. Denucci, and B. Correa. 2005. Mycoflora and occurrence of alternariol and alternariol monomethyl ether in Brazilian sunflower from sowing to harvest. J. Agric. Food Chem. 53:5824-5828. (Pubitemid 41022485)
29. Pryor, B., and R. L. Gilbertson. 2000. Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mtSSU rDNA sequences. Mycol. Res. 104:1312-1321. (Pubitemid 32102793)
30. Scheu, P., M. K. Berghof, and U. Stahl. 1998. Detection of pathogenic and spoilage microorganisms in food with the polymerase chain reaction. Food Microbiol. 15:13-31. (Pubitemid 28129863)
31. Seung-Jo, G., H. Seung-Beum, K. Hee-Wan, Y. Seung-Hun, and R. Jin-Chang. 1997. Phylogenetic relationship of host-specific toxinproducing Alternaria spp. on the basis sequences of internal transcribed spacer in ribosomal DNA. Crop Prot. 39:1-9.
32. Simmons, E. G. 2007. Alternaria, an identification manual. CBS Fungal Biodiversity Centre, Utrecht, The Netherlands.
33. Solfrizzo, M., A. De Girolamo, C. Vitti, K. Tylkowska, J. Grabarkiewicz-Szczesna, D. Szopinska, and H. Dorna. 2005. Toxigenic profile of Alternaria alternata and Alternaria radicina occurring on umbelliferous plants. Food Addit. Contam. 22:302-308. (Pubitemid 40723765)
34. Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599. (Pubitemid 47236692)
35. Vujanovic, V., and M. Labrecque. 2008. Potentially pathogenic and biocontrol Ascomycota associated with green wall structures of basket willow (Salix viminalis L.) revealed by phenotypic characters and ITS phylogeny. Biocontrol 53:413-426. (Pubitemid 351422004)
36. Xu, L., and L. Du. 2006. Direct detection and quantification of Alternaria alternata lycopersici toxins using high-performance liquid chromatography-evaporative light-scattering detection. J. Microbiol. Methods 64:398-405. (Pubitemid 43212815)
37. Zhou, G., W. Z. Whong, T. Ong, and B. Chen. 2000. Development of a fungus-specific PCR assay for detecting low-level fungi in an indoor environment. Mol. Cell. Probes 14:339-348. (Pubitemid 32001059)
38. Zur, G., E. M. Hallerman, R. Sharf, and Y. Kashi. 1999. Development of a polymerase chain reaction-based assay for the detection of Alternaria fungal contamination in food products. J. Food Prot. 62:1191-1197. (Pubitemid 29478348)
39. Zur, G., E. M. Shimoni, E. Hallerman, and Y. Kashi. 2002. Detection of Alternaria fungal contamination in cereal grains by a polymerase chain reaction-based assay. J. Food Prot. 65:1433-1440. (Pubitemid 35022265)