Increased expression, folding and enzyme reaction rate of recombinant human insulin by selecting appropriate leader peptide

Journal of Biotechnology

Volumn 151, Issue 4, 2011, Pages 350-356

  Min, Cheol Ki ^a   Son, Young Jin ^b   Kim, Chang Kyu ^c   Park, Sang Joong ^d   Lee, Jin Won ^a  

^a Sogang University   (South Korea)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^c Konkuk University   (South Korea)

^d Laboratory of Bioengineering   (South Korea)

Author keywords

Enzymatic conversion; Leader peptide; Proinsulin; S sulfonated preproinsulin (SSPPI); Sulfitolysis

Indexed keywords

ENZYMATIC CONVERSIONS; LEADER PEPTIDE; PROINSULIN; S-SULFONATED PREPROINSULIN (SSPPI); SULFITOLYSIS;

AMINO ACIDS; ENZYMES; INSULIN; REACTION RATES;

PEPTIDES;

ARGININE; C PEPTIDE; CYANOGEN BROMIDE; HYBRID PROTEIN; LYSINE; MERCAPTOETHANOL; PLASMID PPT B5KPI; PLASMID PPT B5RPI; PLASMID PPT H27RPI; PLASMID PPT T10RPI; PLASMID PPT T13RPI; PLASMID VECTOR; PREPROINSULIN; RECOMBINANT HUMAN INSULIN; S SULFONATED PREPROINSULIN; SIGNAL PEPTIDE; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME KINETICS; ENZYME MODIFICATION; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; GENETIC TRANSFECTION; HORMONE SYNTHESIS; HYDROPHILICITY; HYDROPHOBICITY; INSULIN SYNTHESIS; INTERMETHOD COMPARISON; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN CONFORMATION; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STRUCTURE;

ESCHERICHIA COLI; GENE EXPRESSION PROFILING; GENETIC VECTORS; HUMANS; INSULIN; PROINSULIN; PROTEIN CONFORMATION; PROTEIN PRECURSORS; PROTEIN REFOLDING; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; TRYPSIN;

EID: 79951550256     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.12.023     Document Type: Article

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