Recombinant human insulin IX. Investigation of factors, influencing the folding of fusion protein-S-sulfonates, biotechnological precursors of human insulin

Protein Expression and Purification

Volumn 26, Issue 2, 2002, Pages 187-193

  Tikhonov, Roman V ^a   Pechenov, Sergey E ^a   Belacheu, Irina A ^a   Yakimov, Sergey A ^a   Klyushnichenko, Vadim E ^b   Tunes, Heloisa ^c   Thiemann, Josef E ^c   Vilela, Luciano ^c   Wulfson, Andrey N ^a  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b BIOTECO   (Canada)

^c Biotechnology Unit   (Brazil)

Author keywords

Circular dichroism; Factors influencing on folding; Redox potential; S sulfonated insulin precursor; Secondary structure

Indexed keywords

ESCHERICHIA COLI;

INSULIN; PROTEIN PRECURSOR; RECOMBINANT PROTEIN; SULFONIC ACID DERIVATIVE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BIOTECHNOLOGY; CHEMISTRY; CIRCULAR DICHROISM; GEL CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; METABOLISM; MOLECULAR GENETICS; PROTEIN SECONDARY STRUCTURE;

AMINO ACID SEQUENCE; BIOTECHNOLOGY; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; HUMANS; INSULIN; MOLECULAR SEQUENCE DATA; PROTEIN PRECURSORS; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SULFONIC ACIDS;

EID: 0036421115     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00531-4     Document Type: Article

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