Expression and folding of an interleukin-2-proinsulin fusion protein and its conversion into insulin by a single step enzymatic removal of the C-peptide and the N-terminal fused sequence

FEBS Letters

Volumn 378, Issue 2, 1996, Pages 171-176

  Castellanos Serra, Lila R ^a   Hardy, Eugenio ^a   Ubieta, Raimundo ^a   Vispo, Nelson S ^a   Fernandez, Cesar ^a   Besada, Vladimir ^a   Falcon, Viviana ^a   Gonzalez, Marta ^a   Santos, Alicia ^a   Perez, Gudelia ^a   Silva, Alejandro ^a   Herrera, Luis ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Enzymatic processing; Proinsulin folding; Recombinant insulin

Indexed keywords

CARBOXYPEPTIDASE B; HYBRID PROTEIN; INSULIN; INTERLEUKIN 2 DERIVATIVE; PROINSULIN; TRYPSIN;

ARTICLE; DRUG MANUFACTURE; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; RECOMBINANT DNA TECHNOLOGY; TECHNIQUE;

ESCHERICHIA COLI;

EID: 17144455014     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/0014-5793(95)01437-3     Document Type: Article

References (11)

1. Frank, B.H., Pettee, J.M., Zimmerman, R.E. and Burck, P.J. (1981) in: Peptides: Proceedings of the Seventh American Peptide Chemistry Symposium, pp. 729-738, (Rich, D. and Gross, E., Eds.), Pierce Chem. Co. Rockford, Il.
2. Williams, D.C., Van Frank, R.M., Muth, W.L. and Burnett, J.P. (1982) Science 215, 687-689.
3. Sung, W.L., Yau, F.L., Zahab, D.M. and Narang, S.A. (1986) Proc. Natl. Acad. Sci. USA 83, 561-565.
4. Chance, R.E. and Frank, B. (1993) Diabetes Care Suppl. 3, 133-142.
5. Belagaje, R.M., Heath, W.F. and Long, H.B. (1992) Eur. Patt. Publication 0 518 587 A2, Eur. Patt. Office Bulletin 92/51.
6. Heath, W., Belagaje, R., Brooke, G., Chance, R., Hoffmann, J., Long, H., Reams, S., Roundtree, C., Shaw, W., Slieker, L., Sundell, K. and DiMarchi, R. (1992) J. Biol. Chem. 267, 419-425.
7. Frank, B., Furman, T.C. and McDonough, J.P. (1993) Eur. Patt. Publication 0557076 A1, Eur. Patt. Office Bulletin 93/34.
8. Novoa, L.I., Machado, J., Jimenez, V., Padron, G., Penton, E., Benitez, J., Estrada, M.P., Herrera, L., Rodriguez, J.L., Garcia, J. and Narciandi, E. (1991) Eur. Patt. Office Bulletin 91/11 (13-03-1991).
9. Jimenez, V., Guimil, R., Ubieta, R., Ochagavia, M.E., Silva, A., Villegas, G. and Herrera, L. (1990) Biotecnología Aplicada 7, 142-152.
10. Hanahan, D.J. (1983) J. Mol. Biol. 166, 557-580.
11. Laemmli, U.K. (1970) Nature 227, 680-685.