Renaturation of human proinsulin - A study on refolding and conversion to insulin

Analytical Biochemistry

Volumn 310, Issue 2, 2002, Pages 148-155

  Winter, Jeannette ^a   Lilie, Hauke ^a   Rudolph, Rainer ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Disulfide bond formation; Oxidative folding; Proinsulin; Proteolysis; Pulse renaturation

Indexed keywords

AMINO ACIDS; COVALENT BONDS; DICHROISM; ESCHERICHIA COLI; PROTEOLYSIS; RECOMBINANT PROTEINS; REDOX REACTIONS; SULFUR COMPOUNDS; UREA;

DISULFIDE BOND FORMATION; ENZYMATIC CONVERSIONS; NATIVE STRUCTURES; OXIDATIVE FOLDING; PROINSULIN; PROTEIN CONCENTRATIONS; REFOLDING PROCESS; RENATURATION;

INSULIN;

CARBOXYPEPTIDASE B; INSULIN; PROINSULIN; RECOMBINANT PROTEIN; TRYPSIN; CARBOXYPEPTIDASE; CYSTEINE; CYSTINE; GUANIDINE; UREA;

ARTICLE; CELL INCLUSION; CIRCULAR DICHROISM; DISULFIDE BOND; ENZYME KINETICS; ESCHERICHIA COLI; OXIDATION REDUCTION POTENTIAL; OXIDATION REDUCTION STATE; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN ISOLATION; PROTEIN RENATURATION; PROTEIN STRUCTURE; TEMPERATURE DEPENDENCE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; METABOLISM; METHODOLOGY; OXIDATION REDUCTION REACTION; PH; PROTEIN DENATURATION; PROTEIN SECONDARY STRUCTURE; TEMPERATURE;

ESCHERICHIA COLI;

CARBOXYPEPTIDASE B; CARBOXYPEPTIDASES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; CYSTEINE; CYSTINE; ENZYME-LINKED IMMUNOSORBENT ASSAY; GUANIDINE; HUMANS; HYDROGEN-ION CONCENTRATION; INSULIN; OXIDATION-REDUCTION; PROINSULIN; PROTEIN DENATURATION; PROTEIN RENATURATION; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; TEMPERATURE; TRYPSIN; UREA;

EID: 0037112617     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00287-7     Document Type: Article

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