Quantification of cholesterol-metabolizing p450s CYP27A1 and CYP46A1 in neural tissues reveals a lack of enzyme-product correlations in human retina but not human brain

Journal of Proteome Research

Volumn 10, Issue 1, 2011, Pages 241-248

  Liao, Wei Li ^a,b   Heo, Gun Young ^c   Dodder, Nathan G ^b,d   Reem, Rachel E ^c   Mast, Natalia ^c   Huang, Suber ^c   DiPatre, Pier Luigi ^d   Turko, Illarion V ^d   Pikuleva, Irina A ^c  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^c CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

Author keywords

Absolute quantification; CYP27A1; CYP46A1; Human brain; Human retina; Mass spectrometry; Membrane protein; Multiple reaction monitoring

Indexed keywords

CHOLESTANETRIOL 26 MONOOXYGENASE; CHOLESTEROL; CYTOCHROME P450; CYTOCHROME P450 46A1; FIBULIN 5; MEMBRANE PROTEIN; PEPTIDE; UNCLASSIFIED DRUG;

BRAIN; CHOLESTEROL METABOLISM; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME ANALYSIS; GAS CHROMATOGRAPHY; HUMAN; HUMAN TISSUE; NERVOUS TISSUE; PRIORITY JOURNAL; PROTEIN PROCESSING; QUANTITATIVE ASSAY; REACTION ANALYSIS; RETINA; TANDEM MASS SPECTROMETRY; TEMPORAL LOBE;

CELL MEMBRANE; CHOLESTENES; CHOLESTEROL; CYTOCHROME P-450 CYP27A1; HUMANS; HYDROXYCHOLESTEROLS; ISOTOPE LABELING; MASS SPECTROMETRY; NITROGEN ISOTOPES; REPRODUCIBILITY OF RESULTS; RETINA; STEROID HYDROXYLASES; TEMPORAL LOBE;

EID: 79951533845     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr1008898     Document Type: Conference Paper

References (32)

1. National Cholesterol Education Program Expert Panel. Third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (adult treatment panel III) final report. Circulation 2002, 106, 3143-3421.
2. Wolozin, B. Cholesterol and the biology of Alzheimer's disease. Neuron 2004, 41, 7-10. (Pubitemid 38068431)
3. van Leeuwen, R.; Klaver, C. C.; Vingerling, J. R.; Hofman, A.; van Duijn, C. M.; Stricker, B. H.; de Jong, P. T. Cholesterol and agerelated macular degeneration: is there a link. Am. J. Ophthalmol. 2004, 137, 750-752. (Pubitemid 38446692)
4. Pikuleva, I. A. Cholesterol-metabolizing cytochromes P450: implications for cholesterol lowering. Expert Opin. Drug Metab. Toxicol. 2008, 4, 1403-1414.
5. Lutjohann, D.; Breuer, O.; Ahlborg, G.; Nennesmo, I.; Siden, A.; Diczfalusy, U.; Bjorkhem, I. Cholesterol homeostasis in human brain: evidence for an age-dependent flux of 24S-hydrohycholesterol from the brain into the circulation. Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 9799-9804. (Pubitemid 26296689)
6. Bjorkhem, I.; Lutjohann, D.; Diczfalusy, U.; Stahle, L.; Ahlborg, G.; Wahren, J. Cholesterol homeostasis in human brain: turnover of 24S-hydroxycholesterol and evidence for a cerebral origin of most of this oxysterol in the circulation. J. Lipid Res. 1998, 39, 1594-1600. (Pubitemid 28377923)
7. Lund, E. G.; Guileyardo, J. M.; Russell, D. W. cDNA cloning of cholesterol 24-hydroxylase, a mediator of cholesterol homeostasis in the brain. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 7238-7243. (Pubitemid 29299645)
8. Bjorkhem, I.; Heverin, M.; Leoni, V.; Meaney, S.; Diczfalusy, U. Oxysterols and Alzheimer's disease. Acta Neurol. Scand. 2006, 114, 43-49. (Pubitemid 44015224)
9. Tserentsoodol, N.; Sztein, J.; Campos, M.; Gordiyenko, N. V.; Fariss, R. N.; Lee, J. W.; Fliesler, S. J.; Rodriguez, I. R. Uptake of cholesterol by the retina occurs primarily via a low density lipoprotein receptor-mediated process. Mol. Vision 2006, 12, 1306-1318. (Pubitemid 44688464)
10. Lee, J. W.; Fuda, H.; Javitt, N. B.; Strott, C. A.; Rodriguez, I. R. Expression and localization of sterol 27-hydroxylase (CYP27A1) in monkey retina. Exp. Eye Res. 2006, 83, 465-469. (Pubitemid 43850940)
11. Bretillon, L.; Diczfalusy, U.; Bjorkhem, I.; Maire, M. A.; Martine, L.; Joffre, C.; Acar, N.; Bron, A.; Creuzot-Garcher, C. Cholesterol- 24S-hydroxylase (CYP46A1) is specifically expressed in neurons of the neural retina. Curr. Eye Res. 2007, 32, 361-366. (Pubitemid 46633860)
12. Ramirez, D. M. O.; Andersson, S.; Russell, D. W. Neuronal expression and subcellular localization of cholesterol 24-hydroxylase in the mouse brain. J. Comp. Neurol. 2008, 507, 1676-1693. (Pubitemid 351468217)
13. Mast, N.; Reem, R.; Bederman, I.; Huang, S.; DiPatre, P. L.; Bjorkhem, I.; Pikuleva, I. A. Cholestenoic acid is an important elimination product of cholesterol in the retina: comparison of retinal cholesterol metabolism to that in the brain. Invest. Ophthalmol. Visual Sci., in press.
14. Anderson, L.; Hunter, C. L. Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins. Mol. Cell. Proteomics 2006, 5, 573-588.
15. Keshishian, H.; Addona, T.; Burgess, M.; Kuhn, E.; Carr, S. A. Quantitative, multiplexed assays for low abundance proteins in plasma by targeted mass spectrometry and stable isotope dilution. Mol. Cell. Proteomics 2007, 6, 2212-2229.
16. Picotti, P.; Bodenmiller, B.; Mueller, L. N.; Domon, B.; Aebersold, R. Full dynamic range proteome analysis of S. cerevisiae by targeted proteomics. Cell 2009, 138, 795-806.
17. Kitteringham, N. R.; Jenkins, R. E.; Lane, C. S.; Elliott, V. L.; Park, B. K. Multiple reaction monitoring for quantitative biomarker analysis in proteomics and metabolomics. J. Chromatogr., B 2009, 877, 1229-1239.
18. Seibert, C.; Davidson, B. R.; Fuller, B. J.; Patterson, L. H.; Griffiths, W. J.; Wang, Y. Multiple-approaches to the identification and quantification of cytochromes P450 in human liver tissue by mass spectrometry. J. Proteome Res. 2009, 8, 1672-1681.
19. Jiang, H.; Ramos, A. A.; Yao, X. Targeted quantification of overexpressed and endogenous cystic fibrosis transmembrane conductance regulator using multiple monitoring tandem mass spectrometry and oxygen stable isotope dilution. Anal. Chem. 2010, 82, 336-342.
20. Liao, W.-L.; Heo, G.-Y.; Dodder, N. G.; Pikuleva, I. A.; Turko, I. V. Optimizing the conditions of a multiple reaction monitoring assay for membrane proteins: quantification of cytochrome P450 11A1 and adrenodoxin reductase in bovine adrenal cortex and retina. Anal. Chem. 2010, 82, 5760-5767.
21. Pikuleva, I. A.; Bjorkhem, I.; Waterman, M. R. Expression, purification, and enzymatic properties of recombinant human cytochrome P450c27 (CYP27). Arch. Biochem. Biophys. 1997, 343, 123-130. (Pubitemid 27274350)
22. Mast, N.; Norcross, R.; Andersson, U.; Shou, M.; Nakayama, K.; Bjorkhem, I.; Pikuleva, I. A. Broad substrate specificity of human cytochrome P450 46A1 which initiates cholesterol degradation in the brain. Biochemistry 2003, 42, 14284-14292. (Pubitemid 37499426)
23. Marley, J.; Lu, M.; Bracken, C. A method for efficient isotopic labeling of recombinant proteins. J. Biomol. NMR 2001, 20, 71-75. (Pubitemid 32519656)
24. Omura, R.; Sato, R. The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J. Biol. Chem. 19 64, 239, 2370-2378.
25. Janecki, D. J.; Bemis, K. G.; Tegeler, T. J.; Sanghani, P. C.; Zhai, L.; Hurley, T. D.; Bosron, W. F.; Wang, M. A multiple reaction monitoring method for absolute quantification of the human liver alcohol dehydrogenase ADH1C1 isoenzyme. Anal. Biochem. 2007, 369, 18-26. (Pubitemid 47313380)
26. Brun, V.; Dupuis, A.; Adrait, A.; Marcellin, M.; Thomas, D.; Court, M.; Vandenesch, F.; Garin, J. Isotope-labeled protein standards: toward absolute quantitative proteomics. Mol. Cell. Proteomics 2007, 6, 2139-2149.
27. Singh, R.; Crow, F. W.; Babic, N.; Lutz, W. H.; Lieske, J. C.; Larson, T. S.; Kumar, R. A liquid chromatography-mass spectrometry method for the quantification of urinary albumin using a novel Quantification of Cholesterol-Metabolizing P450s research articles 15N-isotopically labeled albumin internal standard. Clin. Chem. 2007, 53, 540-542. (Pubitemid 46447959)
28. Pikuleva, I. A.; Mast, N.; Liao, W. L.; Turko, I. V. Studies of membrane topology of mitochondrial cholesterol hydroxylases CYPs 27A1 and 11A1. Lipids 2008, 43, 1127-1132.
29. Mast, N.; Liao, W. L.; Pikuleva, I. A.; Turko, I. V. Combined use of mass spectrometry and heterologous expression for identification of membrane-interacting peptides in cytochrome P450 46A1 and NADPH-cytochrome P450 oxidoreductase. Arch. Biochem. Biophys. 2009, 483, 81-89.
30. von Wachenfeldt, C.; Johnson, E. F. Structures of eukaryotic cytochrome P450 enzymes. In Cytochrome P450: Structure, Mechanism, and Biochemistry; Ortiz de Montellano, P. R., Ed.; Plenum Press: New York, 1995; pp 183-244.
31. Omura, T. Mitochondrial P450s. Chem.-Biol. Interact. 2006, 163, 86-93. (Pubitemid 44572220)
32. Guengerich, F. P.; Turvy, C. G. Comparison of levels of several human microsomal cytochrome P-450 enzymes and epoxide hydrolase in normal and disease states using immunochemical analysis of surgical liver samples. J. Pharmacol. Exp. Ther. 1991, 256, 1189-1194.