Overexpression of Bcl-2 in hepatocytes protects against injury but does not attenuate fibrosis in a mouse model of chronic cholestatic liver disease

Laboratory Investigation

Volumn 91, Issue 2, 2011, Pages 273-282

  Mitchell, Claudia ^a   Mahrouf Yorgov, Meriem ^a   Mayeuf, Alicia ^a   Robin, Marie Anne ^b   Mansouri, Abdellah ^c   Fromenty, Bernard ^b   Gilgenkrantz, Hélène ^a  

^a INSTITUT COCHIN   (France)

^b UNIVERSITÉ DE RENNES 1   (France)

^c CENTRE DE RECHERCHE BIOMÉDICALE BICHAT BEAUJON CRB3   (France)

Author keywords

apoptosis; Bcl 2; cholestasis; fibrosis; inflammation; necrosis; oxydative stress

Indexed keywords

PROTEIN BCL 2;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL DEATH; CELL PROTECTION; CHRONIC LIVER DISEASE; ENZYME ACTIVITY; LIVER CELL; LIVER INJURY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; APOPTOSIS; BILE DUCTS; BLOTTING, WESTERN; CASPASES; CHOLESTASIS, INTRAHEPATIC; ELECTRON TRANSPORT COMPLEX I; HEPATOCYTES; HISTOLOGICAL TECHNIQUES; IN SITU NICK-END LABELING; LIGATION; LIPID PEROXIDATION; MICE; MICE, TRANSGENIC; NECROSIS; PROTO-ONCOGENE PROTEINS C-BCL-2; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 79551595838     PISSN: 00236837     EISSN: 15300307     Source Type: Journal    
DOI: 10.1038/labinvest.2010.163     Document Type: Article

References (32)

1. Faubion WA, Guicciardi ME, Miyoshi H, et al. Toxic bile salts induce rodent hepatocyte apoptosis via direct activation of Fas. J Clin Invest 1999;103:137-145.
2. Canbay A, Higuchi H, Bronk SF, et al. Fas enhances fibrogenesis in the bile duct ligated mouse: a link between apoptosis and fibrosis. Gastroenterology 2002;123:1323-1330.
3. Miyoshi H, Rust C, Roberts PJ, et al. Hepatocyte apoptosis after bile duct ligation in the mouse involves Fas. Gastroenterology 1999;117:669-677.
4. Canbay A, Feldstein A, Baskin-Bey E, et al. The caspase inhibitor IDN- 6556 attenuates hepatic injury and fibrosis in the bile duct ligated mouse. J Pharmacol Exp Ther 2004;308:1191-1196.
5. Kahraman A, Barreyro FJ, Bronk SF, et al. TRAIL mediates liver injury by the innate immune system in the bile duct-ligated mouse. Hepatology (Baltimore, MD) 2008;47:1317-1330.
6. Higuchi H, Bronk SF, Taniai M, et al. Cholestasis increases tumor necrosis factor-related apoptotis-inducing ligand (TRAIL)-R2/DR5 expression and sensitizes the liver to TRAIL-mediated cytotoxicity. J Pharmacol Exp Ther 2002;303:461-467.
7. Gujral JS, Liu J, Farhood A, et al. Reduced oncotic necrosis in Fas receptor-deficient C57BL/6J-lpr mice after bile duct ligation. Hepatology (Baltimore, MD) 2004;40:998-1007.
8. Fickert P, Trauner M, Fuchsbichler A, et al. Oncosis represents the main type of cell death in mouse models of cholestasis. J Hepatol 2005;42: 378-385.
9. Nalapareddy P, Schungel S, Hong JY, et al. The BH3-only protein bid does not mediate death-receptor-induced liver injury in obstructive cholestasis. Am J Pathol 2009;175:1077-1085.
10. Schoemaker MH, Gommans WM, Conde de la Rosa L, et al. Resistance of rat hepatocytes against bile acid-induced apoptosis in cholestatic liver injury is due to nuclear factor-kappa B activation. J Hepatol 2003;39:153-161.
11. Vogel A, Aslan JE, Willenbring H, et al. Sustained phosphorylation of Bid is a marker for resistance to Fas-induced apoptosis during chronic liver diseases. Gastroenterology 2006;130:104-119.
12. Gujral JS, Farhood A, Bajt ML, et al. Neutrophils aggravate acute liver injury during obstructive cholestasis in bile duct-ligated mice. Hepatology (Baltimore, MD) 2003;38:355-363.
13. Lacronique V, Mignon A, Fabre M, et al. Bcl-2 protects from lethal hepatic apoptosis induced by an anti-Fas antibody in mice. Nat Med 1996;2:80-86.
14. De la Coste A, Fabre M, McDonell N, et al. Differential protective effects of Bcl-xL and Bcl-2 on apoptotic liver injury in transgenic mice. Am J Physiol 1999;277(3 Part 1):G702-G708.
15. Mitchell C, Mignon A, Guidotti JE, et al. Therapeutic liver repopulation in a mouse model of hypercholesterolemia. Hum Mol Genet 2000; 9:1597-1602.
16. Mignon A, Guidotti JE, Mitchell C, et al. Selective repopulation of normal mouse liver by Fas/CD95-resistant hepatocytes. Nat Med 1998;4:1185-1188.
17. Hockenbery DM, Oltvai ZN, Yin XM, et al. Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell 1993;75:241-251.
18. Kane DJ, Sarafian TA, Anton R, et al. Bcl-2 inhibition of neural death: decreased generation of reactive oxygen species. Science (New York, NY) 1993;262:1274-1277.
19. Peralta C, Perales JC, Bartrons R, et al. The combination of ischemic preconditioning and liver Bcl-2 overexpression is a suitable strategy to prevent liver and lung damage after hepatic ischemia-reperfusion. Am J Pathol 2002;160:2111-2122.
20. Mitchell C, Robin M.A., Mayeuf A, et al. Protection against hepatocyte mitochondrial dysfunction delays fibrosis progression in mice. Am J Pathol 2009;175:1929-1937.
21. Serviddio G, Pereda J, Pallardo FV, et al. Ursodeoxycholic acid protects against secondary biliary cirrhosis in rats by preventing mitochondrial oxidative stress. Hepatology (Baltimore, MD) 2004;39:711-720.
22. Mitchell C, Couton D, Couty JP, et al. Dual role of CCR2 in the constitution and the resolution of liver fibrosis in mice. Am J Pathol 2009;174:1766-1775.
23. Georgiev P, Jochum W, Heinrich S, et al. Characterization of timerelated changes after experimental bile duct ligation. Br J Surg 2008;95:646-656.
24. Grasl-Kraupp B, Ruttkay-Nedecky B, Koudelka H, et al. In situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: a cautionary note. Hepatology (Baltimore, MD) 1995;21:1465-1468.
25. Szymanska JA. Hepatotoxicity of brominated benzenes: relationship between chemical structure and hepatotoxic effects in acute intoxication of mice. Arch Toxicol 1998;72:97-103.
26. Song M, Song Z, Barve S, et al. Tetrathiomolybdate protects against bile duct ligation-induced cholestatic liver injury and fibrosis. J Pharmacol Exper Therap 2008;325:409-416.
27. Gabele E, Froh M, Arteel GE, et al. TNFalpha is required for cholestasisinduced liver fibrosis in the mouse. Biochem Biophys Res Commun 2009;378:348-353.
28. Kahraman A, Mott JL, Bronk SF, et al. Overexpression of mcl-1 attenuates liver injury and fibrosis in the bile duct-ligated mouse. Dig Dis Sci 2009;54:1908-1917.
29. Ramm GA, Shepherd RW, Hoskins AC, et al. Fibrogenesis in pediatric cholestatic liver disease: role of taurocholate and hepatocyte-derived monocyte chemotaxis protein-1 in hepatic stellate cell recruitment. Hepatology (Baltimore, MD) 2009;49:533-544.
30. Takeda K, Kojima Y, Ikejima K, et al. Death receptor 5 mediatedapoptosis contributes to cholestatic liver disease. Proc Natl Acad Sci USA 2008;105:10895-10900.
31. Sokol RJ, McKim Jr JM, Goff MC, et al. Vitamin E reduces oxidant injury to mitochondria and the hepatotoxicity of taurochenodeoxycholic acid in the rat. Gastroenterology 1998;114:164-174.
32. Sommerfeld A, Reinehr R, Haussinger D. Bile acid-induced epidermal growth factor receptor activation in quiescent rat hepatic stellate cells can trigger both proliferation and apoptosis. J Biol Chem 2009;284: 22173-22183.