Ghrelin protects H9c2 cells from hydrogen peroxide-induced apoptosis through NF-κB and mitochondria-mediated signaling

European Journal of Pharmacology

Volumn 654, Issue 2, 2011, Pages 142-149

  Zhang, Qin ^a   Huang, Wei Dong ^a   Lv, Xue Ying ^a   Yang, Yun Mei ^b  

^a ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b ZHEJIANG UNIVERSITY   (China)

Author keywords

Apoptosis; Ghrelin; H9c2 cells; Oxidative stress

Indexed keywords

CASPASE 3; CASPASE 9; GHRELIN; HYDROGEN PEROXIDE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; PROTEIN BAX; PROTEIN BCL 2; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL PROTECTION; CELL VIABILITY; CONTROLLED STUDY; DOWN REGULATION; EMBRYO; ENZYME ACTIVATION; ENZYME ACTIVITY; HEART MITOCHONDRION; HEART MUSCLE CELL; MITOCHONDRIAL MEMBRANE POTENTIAL; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; RAT; SIGNAL TRANSDUCTION; UPREGULATION;

ANIMALS; APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; CASPASE 3; CELL SURVIVAL; CELLS, CULTURED; GHRELIN; HYDROGEN PEROXIDE; IN SITU NICK-END LABELING; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA, HEART; MYOCYTES, CARDIAC; NF-KAPPA B; OXIDANTS; OXIDATIVE STRESS; PROTO-ONCOGENE PROTEINS C-BCL-2; RATS; REACTIVE OXYGEN SPECIES; SIGNAL TRANSDUCTION;

EID: 79551576278     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejphar.2010.12.011     Document Type: Article

References (49)

1. G. Baldanzi, N. Filigheddu, S. Cutrupi, F. Catapano, S. Bonissoni, and A. Fubini Ghrelin and des-acyl ghrelin inhibit cell death in cardiomyocytes and endothelial cells through ERK1/2 and PI 3-kinase/AKT J. Cell Biol. 159 2002 1029 1037
2. G. Bisi, V. Podio, M.R. Valetto, F. Broglio, G. Bertuccio, G. Aimaretti, E. Pelosi, G. Del Rio, G. Muccioli, and H. Ong Cardiac effects of hexarelin in hypopituitary adults Eur. J. Pharmacol. 381 1999 31 38
3. C.Y. Bowers Unnatural growth hormone-releasing peptide begets natural ghrelin J. Clin. Endocrinol. Metab. 86 2001 1464 1469
4. L. Chang, J. Zhao, J. Yang, Z. Zhang, J. Du, and C. Tang Therapeutic effects of ghrelin on endotoxic shock in rats Eur. J. Pharmacol. 473 2003 171 176
5. L. Chang, Y. Ren, X. Liu, W.G. Li, J. Yang, and B. Geng Protective effects of ghrelin on ischemia/reperfusion injury in the isolated rat heart J. Cardiovasc. Pharmacol. 43 2004 165 170
6. M.T. Crow, K. Mani, Y.J. Nam, and R.N. Kitsis The mitochondrial death pathway and cardiac myocyte apoptosis Circ. Res. 95 2004 957 970
7. V.D. Dixit, E.M. Schaffer, R.S. Pyle, G.D. Collins, S.K. Sakthivel, and R. Palaniappan Ghrelin inhibits leptin- and activation-induced proinflammatory cytokine expression by human monocytes and T cells J. Clin. Invest. 114 2004 57 66
8. 2-induced apoptosis FEBS 275 2008 3136 3144
9. M. Enomoto, N. Nagaya, M. Uematsu, H. Okumura, E. Nakagawa, and F. Ono Cardiovascular and hormonal effects of subcutaneous administration of ghrelin, a novel growth hormone-releasing peptide, in healthy humans Clin. Sci. 105 2003 431 435
10. El.E. Eter, Al.A. Tuwaijiri,, H. Hagar, and M. Arafa In vivo and in vitro antioxidant activity of ghrelin: attenuation of gastric ischemic injury in the rat J. Gastro enterol. Hepatol. 22 2007 1791 1799
11. E.A. Garcia, and M. Korbonits Ghrelin and cardiovascular health Curr. Opin. Pharmacol. 6 2006 142 147
12. C. Gill, R. Mestril, and A. Samali Losing heart: the role of apoptosis in heart disease-a novel therapeutic target? FASEB J. 16 2002 135 146
13. H. Han, H. Long, H. Wang, J. Wang, Y. Zhang, and Z. Wang Progressive apoptotic cell death triggered by transient oxidative insult in H9c2 rat ventricular cells: a novel pattern of apoptosis and the mechanisms Am. J. Physiol. Heart Circ. Physiol. 286 2004 H2169 H2182
14. Y. Hou, J. An, X.R. Hu, B.B. Sun, and J. Lin Ghrelin inhibits interleukin-8 production induced by hydrogen peroxide in A549 cells via NF-κB pathway Int. Immunopharmacol. 9 2009 120 126
15. D. Iarussi, P. Indolfi, and F. Casale Recent advances in the prevention of anthracycline cardiotoxicity in childhood Curr. Med. Chem. 8 2001 1649 1660
16. M.J. Iglesias, R. Pineiro, M. Blanco, R. Gallego, C. Dieguez, O. Gualillo, J.R. Gonzalez-Juanatey, and F. Laqo Growth hormone releasing peptide (ghrelin) is synthesized and secreted by cardiomyocytes Cardiovasc. Res. 62 2004 481 488
17. A. Inu Ghrelin: an orexigenic and somatotrophic signal from the stomach Nat. Rev. Neurosci. 2 2001 551 560
18. S.O. Iseri, G. Sener, B. Saglam, F. Ercan, N. Gedik, and B.C. Yegen Ghrelin alleviates biliary obstruction-induced chronic hepatic injury in rats Regul. Pept. 146 2008 73 79
19. A. Kawczynska-Drozdz, R. Olszanecki, J. Jawien, T. Brzozowski, W.W. Pawlik, and R. Korbut Ghrelin inhibits vascular superoxide production in spontaneously hypertensive rats Am. J. Hypertens. 19 2006 764 767
20. B.W. Kimes, and B.L. Brandt Properties of a clonal muscle cell line from rat heart Exp. Cell Res. 98 1976 367 381
21. M. Kojima, H. Hosoda, Y. Date, M. Nakazato, H. Matsuo, and K. Kangawa Ghrelin is a growth-hormone-releasing acylated peptide from stomach Nature 402 1999 656 660
22. W.G. Li, D. Gavrila, X. Liu, L. Wang, S. Gunnlaugsson, and L.L. Stoll Ghrelin inhibits proinflammatory responses and nuclear factor-kappaB activation in human endothelial cells Circulation 109 2004 2221 2226
23. K. Liu, W.W. Zhang, L. Liu, D.K. He, G.M. Zhou, L.N. Zhou, and R.M. Hu Ghrelin inhibits apoptosis induced by high glucose and sodium palmitate in adult rat cardiomyocytes through the PI3K-Akt signaling pathway Regul. Pept. 155 2009 62 69
24. V. Locatelli, G. Rossoni, F. Schweiger, A. Torsello, V. De Gennaro Colonna, M. Bernareggi, R. Deghenghi, E.E. Muller, and F. Berti Growth hormone-independent cardioprotective effects of hexarelin in the rat Endocrinology 140 1999 4024 4031
25. K. Matsumura, T. Tsuchihashi, K. Fujii, I. Abe, and M. Iida Central ghrelin modulates sympathetic activity in conscious rabbits Hypertension 40 2002 694 699
26. H. Murata, Y. Ihara, H. Nakamura, J. Yodoi, K. Sumikawa, and T. Kondo Glutaredoxin exerts an antiapoptotic effect by regulating the redox state of Akt J. Biol. Chem. 278 2003 50226 50233
27. N. Nagaya, K. Miyatake, M. Uematsu, M. Yamagishi, H. Hosoda, H. Oya, Y. Hayashi, and K. Kangawa Hemodynamic and hormonal effects of human ghrelin in healthy volunteers Am. J. Physiol. Regul. Integr. Comp. Physiol. 280 2001 R1483 R1487
28. N. Nagaya, K. Miyatake, M. Uematsu, H. Oya, W. Shimizu, and H. Hosoda Hemodynamic, renal, and hormonal effects of ghrelin infusion in patients with chronic heart failure J. Clin. Endocrinol. Metab. 86 2001 5854 5859
29. M. Nakazato, N. Murakami, Y. Date, M. Kojima, H. Matsuo, K. Kangawa, and S. Matsukura A role for ghrelin in the central regulation of feeding Nature 409 2001 194 198
30. T.G. Neilan, S.L. Blake, and F. Ichinose Disruption of nitric oxide synthase 3 protects against the cardiac injury, dysfunction, and mortality induced by doxorubicin Circulation 116 2007 506 514
31. M. O'Brien, J.A. Woods, S.A.O. Aherne, and Y.C. Callaghan Cytotoxicity, genotoxicity and oxidative reaction in cell-culture models: modulatory effects of phytochemicals Biochem. Soc. Trans. 28 2000 22 26
32. B.D. Obay, E. Tasdemir, C. Tumer, H.M. Bilgin, and M. Atmaca Dose dependent effects of ghrelin on pentylenetetrazole-induced oxidative stress in a rat seizure model Peptides 29 2008 448 455
33. F. Rossi, C. Bertone, S. Petricca, and V. Santiemma Ghrelin inhibits angiotensin II induced migration of human aortic endothelial cells Atherosclerosis 192 2007 291 297
34. Y. Shimizu, N. Nagaya, Y. Teranishi, M. Imazu, H. Yamamoto, and T. Shokawa Ghrelin improves endothelial dysfunction through growth hormone-independent mechanisms in rats. Biochem. Biophys. Res. Commun. 310 2003 830 835
35. C. Su, K. Chong, K. Edelstein, S. Lille, R. Khardori, and C. Lai Constitutive hsp70 attenuates hydrogen peroxide-induced membrane lipid peroxidation Biochem. Biophys. Res. Commun. 265 1999 279 284
36. M.S. Suleiman, A.P. Halestrap, and E.J. Griffiths Mitochondria: a target for myocardial protection Pharmacol. Ther. 89 2001 29 46
37. G. Takemura, and H. Fujiwara Morphological aspects of apoptosis in heart diseases J. Cell. Mol. Med. 10 2006 56 75
38. G. Takemura, and H. Fujiwara Doxorubicin-induced cardiomyopathy from the cardiotoxic mechanisms to management Prog. Cardiovasc. Dis. 49 2007 330 352
39. M. Tesauro, F. Schinzari, M. Iantorno, S. Rizza, D. Melina, and D. Lauro Ghrelin improves endothelial function in patients with metabolic syndrome Circulation 112 2005 2986 2992
40. A. Tivesten, E. Bollano, K. Caidahl, V. Kujacic, X.Y. Sun, T. Hedner, A. Hjalmarson, B.A. Bengtsson, and J. Isgaard The growth hormone secretagogue hexarelin improves cardiac function in rats after experimental myocardial infarction Endocrinology 141 2000 60 66
41. M. Tschop, D.L. Smiley, and M.L. Heiman Ghrelin induces adiposity in rodents Nature 407 2000 908 913
42. N.A. Turner, F. Xia, G. Azhar, X. Zhang, L. Liu, and J.Y. Wei Oxidative stress induces DNA fragmentation and caspase activation via the JNK pathway in H9c2 cardiac muscle cells J. Mol. Cell. Cardiol. 30 1998 1789 1801
43. A.J. Van der Lely, M. Tscḧ op, M.L. Heiman, and E. Ghigo Biological, physiological, pathophysiological, and pharmacological aspects of ghrelin Endocr. Rev. 25 2004 426 457
44. K.B. Wallace Doxorubicin-induced cardiac mitochondrionopathy Pharmacol. Toxicol. 93 2003 105 115
45. G.L. Wei, D. Gavrila, X.B. Liu, L.X. Wang, and Skuli Gunnlaugsson Ghrelin inhibits proinflammatory responses and nuclear factor-κB activation in human endothelial cells Circulation 11 2004 2221 2226
46. K.E. Wiley, and A.P. Davenport Comparison of vasodilators in human internal mammary artery: ghrelin is a potent physiological antagonist of endothelin-1 Br. J. Pharmacol. 136 2002 1146 1152
47. Z. Xu, W. Wu, X. Zhang, and G. Liu Endogenous ghrelin increases in doxorubicin induced heart failure rats J. Endocrinol. Invest. 30 2007 117 125
48. Z.W. Xu, S.Q. Lin, and W.K. Wu Ghrelin prevents doxorubicin-induced cardiotoxicity through TNF-alpha/NF-κB pathways and mitochondrial protective mechanisms Toxicology 247 2008 133 138
49. C. Yasuoka, Y. Ihara, S. Ikeda, Y. Miyahara, T. Kondo, and S. Kohno Antiapoptotic activity of Akt is downregulated by Ca2+ in myocardiac H9c2 cells. Evidence of Ca(2+)-dependent regulation of protein phosphatase 2Ac J. Biol. Chem. 279 2004 51182 51192