Production of hepatopoietin Cn in high-cell-density cultures of recombinant Escherichia coli and detection of its antioxygen activity

Molecular Biotechnology

Volumn 47, Issue 2, 2011, Pages 111-119

  Liu, Yang ^a,b   Li, Xiao Li ^c   Zhang, Dong Dong ^a   Wang, Qiang ^c   Liu, Yong ^a   Liu, Dong Mei ^a   Wu, Chu Tse ^a,b   Cui, Chun Ping ^a  

^a BEIJING INSTITUTE OF RADIATION MEDICINE   (China)

^b TIANJIN UNIVERSITY   (China)

^c 309TH HOSPITAL OF PLA   (China)

Author keywords

Antioxygen; Hepatopoietin Cn; High cell density fermentation; Recombinant protein; Renaturation

Indexed keywords

ANTIOXYGEN; HEPATOPOIETIN CN; HIGH-CELL-DENSITY FERMENTATION; RECOMBINANT PROTEIN; RENATURATION;

AMINO ACIDS; BIOACTIVITY; CELL CULTURE; ESCHERICHIA COLI; ETHANOL; FERMENTATION; PURIFICATION; SCALES (WEIGHING INSTRUMENTS);

PROTEINS;

ALCOHOL; HEPATOPOIETIN; HEPATOPOIETIN CN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; BIOREACTOR; CELL CULTURE; CELL DENSITY; CHROMATOGRAPHY; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; FED BATCH FERMENTATION; LARGE SCALE PRODUCTION; LIVER CELL DAMAGE; LIVER PROTECTION; NONHUMAN; OXIDATIVE STRESS; PILOT STUDY; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN RENATURATION; SOLUBILIZATION;

ANTIOXIDANTS; BASE SEQUENCE; CELL CULTURE TECHNIQUES; ESCHERICHIA COLI; HEPATOCYTE GROWTH FACTOR; HEPATOCYTES; HUMANS; INCLUSION BODIES; NUCLEAR PROTEINS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 79551535519     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9318-x     Document Type: Article

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