Expression of the pyranose 2-oxidase from Trametes pubescens in Escherichia coli and characterization of the recombinant enzyme

Journal of Biotechnology

Volumn 120, Issue 4, 2005, Pages 387-395

  Marešová, Helena ^a   Večerek, Branislav ^a   Hradská, Marcela ^a   Libessart, Nathalie ^b   Bečka, Stanislav ^a   Saniez, Marie Helene ^b   Kyslík, Pavel ^a  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

^b ROQUETTE Microbiology and Genetic Department   (France)

Author keywords

Heterologous expression; Inclusion bodies; Pyranose 2 oxidase; Recombinant protein

Indexed keywords

DNA; ENZYMES; ESCHERICHIA COLI; GENETIC ENGINEERING; PROTEINS; SYNTHESIS (CHEMICAL);

HETEROGENEOUS EXPRESSIONS; INCLUSION BODIES; PYRANOSE-2 OXIDASE; RECOMBINANT PROTEINS;

ENZYME KINETICS;

COMPLEMENTARY DNA; OXIDOREDUCTASE; RECOMBINANT ENZYME;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; CELL INCLUSION; COMPARATIVE STUDY; ESCHERICHIA COLI; GENE SEQUENCE; GENETIC CODE; GENETIC TRAIT; KINETICS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYSICAL CHEMISTRY; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PURIFICATION; SYNTHESIS; TRAMETES;

BASIDIOMYCOTA; CARBOHYDRATE DEHYDROGENASES; ESCHERICHIA COLI; FUNGAL PROTEINS; GENE EXPRESSION; INCLUSION BODIES; KINETICS; PROTEIN FOLDING; SPECIES SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; TRAMETES; TRAMETES OCHRACEA; TRAMETES PUBESCENS;

EID: 27744471883     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2005.06.021     Document Type: Article

References (21)

1. L. Akileswaran, B.J. Brock, J.L. Cereghino, and M.H. Gold 1,4-Benzoquinone reductase from Phanerochaete chrysosporium: cDNA cloning and regulation of expression Appl. Environ. Microbiol. 65 1999 415 421
2. J.J. Chalmers, E. Kim, J.N. Telford, E.Y. Wong, W.C. Tacon, M.L. Shuler, and D.B. Wilson Effects of temperature on Escherichia coli overproducing β-lactamase or human epidermal growth factor Appl. Environ. Microbiol. 56 1990 104 111
3. 2 during wood degradation by Phanerochaete chrysosporium, Trametes versicolor, and Oudemansiella mucida Appl. Environ. Microbiol. 60 1994 2524 2532
4. H.J. Danneel, E. Rössner, A. Zeeck, and F. Giffhorn Purification and characterization of a pyranose oxidase from the basidiomycete Peniophora gigantea and chemical analyses of its reaction products Eur. J. Biochem. 214 1993 795 802
5. S. Freimund, A. Huwig, F. Giffhorn, and S. Köpper Rare keto-aldoses from enzymatic oxidation: substrates and oxidation products of pyranose 2-oxidase Chem. Eur. J. 4 1998 2442 2455
6. F. Giffhorn Fungal pyranose oxidases: occurrence, properties and biotechnical applications in carbohydrate chemistry Appl. Microbiol. Biotechnol. 54 2000 727 740
7. P. Halada, C. Leitner, P. Sedmera, D. Haltrich, and J. Volc Identification of the covalent flavin adenine dinucleotide-binding region in pyranose 2-oxidase from Trametes multicolor Anal. Biochem. 314 2003 235 242
8. B.C. Hallberg, C. Leitner, D. Haltrich, and C. Divne Crystal structure of the 270 kDa homotetrameric lignin-degrading enzyme pyranose 2-oxidase J. Mol. Biol. 341 2004 781 796
9. H. Ichinose, H. Wariishi, and H. Tanaka Identification and heterologous expression of the cytochrome P450 oxidoreductase from white-rot basidiomycete Coriolus versicolor Appl. Microbiol. Biotechnol. 59 2002 658 664
10. Y. Iimura, and K. Tatsumi Isolation of mRNA indused by a hazardous chemical in white-rot fungus, Coriolus versicolor, by differential display FEBS Lett. 421 1997 370 374
11. M. Kotik, M. Kočanová, H. Marešová, and P. Kyslík High-level expression of a fungal pyranose oxidase in high cell-density fed-batch cultivations of Escherichia coli using lactose as inducer Protein Expr. Purif. 36 2004 61 69
12. C. Leitner, J. Volc, and D. Haltrich Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor Appl. Environ. Microbiol. 67 2001 3636 3644
13. Y. Machida, and T. Nakanishi Purification and properties of pyranose oxidase from Coriolus versicolor Agric. Biol. Chem. 48 1984 2463 2470
14. H. Nielsen, and A. Krogh Prediction of signal peptides and signal anchors by a hidden Markov model. Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology (ISMB 6) 1998 AAAI Press Menlo Park, California 122 130
15. I. Nishimura, O. Kimiharu, and Y. Koyama Cloning and expression of pyranose oxidase cDNA from Coriolus versicolor in Escherichia coli J. Biotechnol. 2 1996 11 20
16. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual second ed. 1989 Cold Spring Harbor Laboratory Cold Spring Harbor, NY
17. L. Strandberg, and S.O. Enfors Factors influencing inclusion body formation in the production of a fused protein in Escherichia coli Appl. Environ. Microbiol. 57 1991 1669 1674
18. Y. Takakura, and S. Kuwata Purification, characterization, and molecular cloning of a pyranose oxidase from the fruit body of the basidiomycete, Tricholoma matsutake Biosci. Biotechnol. Biochem. 67 12 2003 2598 2607
19. B. Večerek, H. Marešová, M. Kočanová, and P. Kyslík Molecular cloning and expression of the pyranose 2-oxidase cDNA from Trametes ochracea MB49 in Escherichia coli Appl. Microbiol. Biotechnol. 64 2004 525 530
20. J. Volc, and K.E. Eriksson Pyranose 2-oxidase from Phanerochaete chrysosporium Methods Enzymol. 161 1988 316 322
21. J. Volc, P. Sedmera, V. Havlíček, V. Př ikrylová, and G. Daniel Conversion of d-glucose to d-erythro-hexos-2,3- diulose (2,3-diketo-d-glucose) by enzyme preparations from the basidiomycetes Oudemansiella mucida Carbohydr. Res. 278 1995 59 70