Renaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli

Journal of Biotechnology

Volumn 94, Issue 2, 2002, Pages 185-194

  Vallejo, Luis Felipe ^a   Brokelmann, Maren ^a   Marten, Sabine ^a   Trappe, Susanne ^a   Cabrera Crespo, Joaquin ^a   Hoffmann, Andrea ^a   Gross, Gerhard ^a   Weich, Herbert A ^a   Rinas, Ursula ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

E. coli; In vitro refolding; Purification; rhBMP 2

Indexed keywords

AFFINITY CHROMATOGRAPHY; BIOMASS; CELLS; DIMERIZATION; PROTEINS; PURIFICATION;

INCLUSION BODIES;

ESCHERICHIA COLI;

ALKALINE PHOSPHATASE; RECOMBINANT BONE MORPHOGENETIC PROTEIN 2;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; BACTERIUM CULTURE; BONE REGENERATION; CELL DENSITY; CONTROLLED STUDY; DILUTION; DIMERIZATION; ENZYME ACTIVITY; ESCHERICHIA COLI; MICROBIAL BIOMASS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN RENATURATION; TEMPERATURE;

BIOREACTORS; BONE MORPHOGENETIC PROTEINS; CULTURE MEDIA; DIMERIZATION; ESCHERICHIA COLI; GENETIC ENGINEERING; INCLUSION BODIES; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN RENATURATION; RECOMBINANT PROTEINS; TRANSFORMING GROWTH FACTOR BETA;

ESCHERICHIA COLI;

EID: 0037187447     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(01)00425-4     Document Type: Article

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