High-level expression of a fungal pyranose oxidase in high cell-density fed-batch cultivations of Escherichia coli using lactose as inducer

Protein Expression and Purification

Volumn 36, Issue 1, 2004, Pages 61-69

  Kotik, Michael ^a   Kočanová, Marcela ^a   Marešová, Helena ^a   Kyslík, Pavel ^a  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

Author keywords

Fed batch; Inclusion bodies; Lactose; Purification; Recombinant pyranose oxidase

Indexed keywords

FUNGAL PROTEIN; LACTOSE; OXIDOREDUCTASE; PYRANOSE OXIDASE; RECOMBINANT PROTEIN;

ARTICLE; BASIDIOMYCETES; BIOSYNTHESIS; DRUG EFFECT; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; KINETICS;

BASIDIOMYCOTA; CARBOHYDRATE DEHYDROGENASES; ESCHERICHIA COLI; FUNGAL PROTEINS; GENE EXPRESSION REGULATION; KINETICS; LACTOSE; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

BASIDIOMYCOTA; ESCHERICHIA COLI; TRAMETES; TRAMETES OCHRACEA;

EID: 3042591780     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.02.011     Document Type: Article

References (35)

1. Artolozaga M.J., Kubátová E., Volc J., Kalisz H.M. Pyranose 2-oxidase from Phanerochaete chrysosporium - further biochemical characterization. Appl. Microbiol. Biotechnol. 47:1997;508-514
2. Freimund S., Huwig A., Giffhorn F., Köpper S. Rare keto-aldoses from enzymatic oxidation: substrates and oxidation products of pyranose 2-oxidase. Chem. Eur. J. 4:1998;2442-2455
3. Leitner C., Volc J., Haltrich D. Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor. Appl. Environ. Microbiol. 67:2001;3636-3644
4. Giffhorn F. Fungal pyranose oxidases: occurrence, properties and biotechnical applications in carbohydrate chemistry. Appl. Microbiol. Biotechnol. 54:2000;727-740
5. Giffhorn F., Köpper S., Huwig A., Freimund S. Rare sugars and sugar-based synthons by chemo-enzymatic synthesis. Enzyme Microb. Technol. 27:2000;734-742
6. Danneel H.J., Rössner E., Zeeck A., Giffhorn F. Purification and characterization of a pyranose oxidase from the basidiomycete Peniophora gigantea and chemical analyses of its reaction products. Eur. J. Biochem. 214:1993;795-802
7. Schäfer A., Bieg S., Huwig A., Kohring G.W., Giffhorn F. Purification by immunoaffinity chromatography, characterization, and structural analysis of a thermostable pyranose oxidase from the white-rot fungus Phlebiopsis gigantean. Appl. Environ. Microbiol. 62:1996;2586-2592
8. Shin K.S., Youn H.D., Han Y.H., Kang S.O., Hah Y.C. Purification and characterisation of D-glucose oxidase from white-rot fungus Pleurotus ostreatus. Eur. J. Biochem. 215:1993;747-752
9. Costa-Ferreira M., Couto A. High ionic strength tolerance of pyranose oxidase from Trametes versicolor and its purification. Process Biochem. 38:2003;1019-1023
10. Volc J., Kubátová E., Daniel G., Sedmera P., Haltrich D. Screening of basidiomycete fungi for the quinone-dependent sugar C-2/C-3 oxidoreductase, pyranose dehydrogenase, and properties of the enzyme from Macrolepiota rhacodes. Arch. Microbiol. 176:2001;178-186
11. Leitner C., Haltrich D., Nidetzky B., Prillinger H., Kulbe K.D. Production of a novel pyranose 2-oxidase by basidiomycete Trametes multicolor. Appl. Biochem. Biotechnol. 70-72:1998;237-248
12. Furuya Y., Yamada H., Izumi Y. Production of pyranose oxidase by basidiomycetous fungus No. 52. J. Ferment. Bioeng. 76:1993;532-534
13. Nishimura I., Okada K., Koyama Y. Cloning and expression of pyranose oxidase cDNA from Coriolus versicolor in Escherichia coli. J. Biotechnol. 52:1996;11-20
14. S. Christensen, S.F. Lassen, P. Schneider, US Patent 6146865, 2000
15. Takakura Y., Kuwata S. Purification, characterization, and molecular cloning of a pyranose oxidase from the fruit body of the basidiomycete Tricholoma matsutake. Biosci. Biotechnol. Biochem. 67:2003;2598-2607
16. B. Večerek, H. Marešová, M. Kočanová, P. Kyslík, Molecular cloning and expression of the pyranose 2-oxidase cDNA from Trametes ochracea MB49 in Escherichia coli, Appl. Microbiol. Biotechnol., 2004, in press
17. Strobel R.J., Sullivan G.R. Experimental design for improvement of fermentations. Demain A.L., Davies J.E. Manual of Industrial Microbiology and Biotechnology. second ed. 1999;80-93 ASM Press, Washington, DC
18. Lee J., Lee S.Y., Park S., Middelberg A.P.J. Control of fed-batch fermentations. Biotechnol. Adv. 17:1999;29-48
19. Lee S.J., Kim I.C., Kim D.M., Bae K.H., Byun S.M. High level secretion of recombinant staphylokinase into periplasm of Escherichia coli. Biotechnol. Lett. 20:1998;113-116
20. Vohradský J., Marešová H., Večerek B., Kyslík P. Determination of plasmid copy number in continuous culture by fluorescence densitometry and image analysis. Methods Mol. Cell. Biol. 5/6:1990;223-233
21. Makrides S.C. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 60:1996;512-538
22. Kaprálek F., Ječmen P., Sedláček J., Fábry M., Zadražil S. Fermentation conditions for high-level expression of the tac-promoter-controlled calf prochymosin cDNA in Escherichia coli HB101. Biotechnol. Bioeng. 37:1991;71-79
23. Neubauer P., Hofmann K., Holst O., Mattiasson B., Kruschke P. Maximizing the expression of a recombinant gene in Escherichia coli by manipulation of induction time using lactose as inducer. Appl. Microbiol. Biotechnol. 36:1992;739-744
24. Hoffman B.J., Broadwater J.A., Johnson P., Harper J., Fox B.G., Kenealy W.R. Lactose fed-batch overexpression of recombinant metalloproteins in Escherichia coli BL21(DE3): process control yielding high levels of metal-incorporated, soluble protein. Protein Expr. Purif. 6:1995;646-654
25. Donovan R.S., Robinson C.W., Glick B.R. Optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter. J. Ind. Microbiol. 16:1996;145-154
26. Gombert A.K., Kilikian B.V. Recombinant gene expression in Escherichia coli cultivation using lactose as inducer. J. Biotechnol. 60:1998;47-54
27. Nordberg Karlsson E., Holst O., Tocaj A. Efficient production of truncated thermostable xylanases from Rhodothermus marinus in Escherichia coli fed-batch cultures. J. Biosci. Bioeng. 87:1999;598-606
28. Hwang T.S., Fu H.M., Lin L.L., Hsu W.H. High-level expression of Trigonopsis variabilis D-amino acid oxidase in Escherichia coli using lactose as inducer. Biotechnol. Lett. 22:2000;655-658
29. Snyder L., Champness W. Molecular Genetics of Bacteria. 1997;ASM Press, Washington, DC
30. Saier M.H., Crasnier M. Inducer exclusion and the regulation of sugar transport. Res. Microbiol. 147:1996;482-489
31. Yoon S.K., Kang W.K., Park T.H. Fed-batch operation of recombinant Escherichia coli containing trp promoter with controlled specific growth rate. Biotechnol. Bioeng. 43:1994;995-999
32. Lee J., Lee S.Y., Park S. Fed-batch culture of Escherichia coli W by exponential feeding of sucrose as a carbon source. Biotechnol. Tech. 11:1997;59-62
33. Lee S.Y. High cell-density culture of Escherichia coli. Trends Biotechnol. 14:1996;98-105
34. Schein C.H., Noteborn M.H.M. Formation of soluble recombinant proteins in Escherichia coli is favored by lower growth temperature. Bio/Technology. 6:1988;291-294
35. 2 during wood degradation by Phanerochaete chrysosporium, Trametes versicolor, and Oudemansiella mucida. Appl. Environ. Microbiol. 60:1994;2524-2532