High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins

Analytical Biochemistry

Volumn 410, Issue 2, 2011, Pages 272-280

  Berridge, Georgina ^a   Chalk, Rod ^a   D'Avanzo, Nazzareno ^a,b   Dong, Liang ^a   Doyle, Declan ^a,c   Kim, Jung In ^a,d   Xia, Xiaobing ^a   Burgess Brown, Nicola ^a   Deriso, Antonio ^a   Carpenter, Elisabeth Paula ^a   Gileadi, Opher ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c TRINITY COLLEGE DUBLIN   (Ireland)

^d ETH ZURICH   (Switzerland)

Author keywords

Detergent; Electrospray ionization mass spectrometry; HPLC; Intact mass analysis; Integral membrane protein; Isocratic elution; LC MS; Methanol

Indexed keywords

DETERGENTS; ELECTROSPRAY IONIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHANOL; SOAPS (DETERGENTS);

ELECTROSPRAY IONIZATION MASS SPECTROMETRY; HPLC; INTEGRAL MEMBRANE PROTEINS; ISOCRATIC ELUTION; LC-MS; MASS ANALYSIS;

PROTEINS;

DETERGENT; MEMBRANE PROTEIN; METHANOL; PROTEIN ABCB10; PROTEIN HVCN1; PROTEIN KCNJ12; PROTEIN SC4MOL; PROTEIN SCD; PROTEIN SLC22A18; PROTEIN ZMPSTE24; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; SENSITIVITY ANALYSIS; SEPARATION TECHNIQUE;

EID: 79551524680     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.11.008     Document Type: Article

References (23)

1. A.L. Hopkins, and C.R. Groom The druggable genome Nat. Rev. Drug Discov. 1 2002 727 730
2. S.H. White Biophysical dissection of membrane proteins Nature 459 2009 344 346
3. E.P. Carpenter, K. Beis, A.D. Cameron, and S. Iwata Overcoming the challenges of membrane protein crystallography Curr. Opin. Struct. Biol. 18 2008 581 586
4. F. Mancia, and J. Love High-throughput expression and purification of membrane proteins J. Struct. Biol. 172 2010 85 93
5. S. Graslund, P. Nordlund, J. Weigelt, B.M. Hallberg, J. Bray, O. Gileadi, S. Knapp, U. Oppermann, C. Arrowsmith, R. Hui, J. Ming, S. dhe-Paganon, H.W. Park, A. Savchenko, A. Yee, A. Edwards, R. Vincentelli, C. Cambillau, R. Kim, S.H. Kim, Z. Rao, Y. Shi, T.C. Terwilliger, C.Y. Kim, L.W. Hung, G.S. Waldo, Y. Peleg, S. Albeck, T. Unger, O. Dym, J. Prilusky, J.L. Sussman, R.C. Stevens, S.A. Lesley, I.A. Wilson, A. Joachimiak, F. Collart, I. Dementieva, M.I. Donnelly, W.H. Eschenfeldt, Y. Kim, L. Stols, R. Wu, M. Zhou, S.K. Burley, J.S. Emtage, J.M. Sauder, D. Thompson, K. Bain, J. Luz, T. Gheyi, F. Zhang, S. Atwell, S.C. Almo, J.B. Bonanno, A. Fiser, S. Swaminathan, F.W. Studier, M.R. Chance, A. Sali, T.B. Acton, R. Xiao, L. Zhao, L.C. Ma, J.F. Hunt, L. Tong, K. Cunningham, M. Inouye, S. Anderson, H. Janjua, R. Shastry, C.K. Ho, D. Wang, H. Wang, M. Jiang, G.T. Montelione, D.I. Stuart, R.J. Owens, S. Daenke, A. Schutz, U. Heinemann, S. Yokoyama, K. Bussow, K.C. Gunsalus, Protein production and purification, Nat. Methods 5 (2008) 135-46.
6. P. Savitsky, J. Bray, C.D. Cooper, B.D. Marsden, P. Mahajan, N.A. Burgess-Brown, and O. Gileadi High-throughput production of human proteins for crystallization: the SGC experience J. Struct. Biol. 172 2010 3 13
7. M. Cadene, and B.T. Chait A robust, detergent-friendly method for mass spectrometric analysis of integral membrane proteins Anal. Chem. 72 2000 5655 5658
8. H. Takayama, P. Chelikani, P.J. Reeves, S. Zhang, and H.G. Khorana High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81 PLoS One 3 2008 e2314
9. J.P. Whitelegge HPLC and mass spectrometry of intrinsic membrane proteins Methods Mol. Biol. 251 2004 323 340
10. J.P. Whitelegge, C.B. Gundersen, and K.F. Faull Electrospray-ionization mass spectrometry of intact intrinsic membrane proteins Protein Sci. 7 1998 1423 1430
11. + (KCNJ) channels in Saccharomyces cerevisiae Protein Expr. Purif. 71 2010 115 121
12. + channel Kir(2.1b) is inhibited by direct protein kinase C-dependent regulation in human isolated cardiomyocytes and in an expression system Circulation 106 2002 1493 1499
13. S. Huang, R.C. Elliott, P.S. Liu, R.K. Koduri, J.L. Weickmann, J.H. Lee, L.C. Blair, P. Ghosh-Dastidar, R.A. Bradshaw, and K.M. Bryan Specificity of cotranslational amino-terminal processing of proteins in yeast Biochemistry 26 1987 8242 8246
14. C.H. Borchers, R. Thapar, E.V. Petrotchenko, M.P. Torres, J.P. Speir, M. Easterling, Z. Dominski, and W.F. Marzluff Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem-loop-binding proteins that contributes to high-affinity RNA binding Proc. Natl. Acad. Sci. USA 103 2006 3094 3099
15. F. Sherman, J.W. Stewart, and S. Tsunasawa Methionine or not methionine at the beginning of a protein BioEssays 3 1985 27 31
16. S. Tsunasawa, J.W. Stewart, and F. Sherman Amino-terminal processing of mutant forms of yeast iso-1-cytochrome c. The specificities of methionine aminopeptidase and acetyltransferase J. Biol. Chem. 260 1985 5382 5391
17. J. le Coutre, J.P. Whitelegge, A. Gross, E. Turk, E.M. Wright, H.R. Kaback, and K.F. Faull Proteomics on full-length membrane proteins using mass spectrometry Biochemistry 39 2000 4237 4242
18. N.P. Barrera, N. Di Bartolo, P.J. Booth, and C.V. Robinson Micelles protect membrane complexes from solution to vacuum Science 321 2008 243 246
19. N.P. Barrera, S.C. Isaacson, M. Zhou, V.N. Bavro, A. Welch, T.A. Schaedler, M.A. Seeger, R.N. Miguel, V.M. Korkhov, H.W. van Veen, H. Venter, A.R. Walmsley, C.G. Tate, and C.V. Robinson Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions Nat. Methods 6 2009 585 587
20. L.N. Jones, S.A. Baldwin, P.J. Henderson, and A.E. Ashcroft Defining topological features of membrane proteins by nanoelectrospray ionisation mass spectrometry Rapid Commun. Mass Spectrom. 24 2010 276 284
21. H.T. Lin, V.N. Bavro, N.P. Barrera, H.M. Frankish, S. Velamakanni, H.W. van Veen, C.V. Robinson, M.I. Borges-Walmsley, and A.R. Walmsley MacB ABC transporter is a dimer whose ATPase activity and macrolide-binding capacity are regulated by the membrane fusion protein MacA J. Biol. Chem. 284 2009 1145 1154
22. F. Sobott, and C.V. Robinson Protein complexes gain momentum Curr. Opin. Struct. Biol. 12 2002 729 734
23. C.C. Wu, and J.R. Yates 3rd. The application of mass spectrometry to membrane proteomics Nat. Biotechnol. 21 2003 262 267