The N-terminal tail of hERG contains an amphipathic α-helix that regulates channel deactivation

PLoS ONE

Volumn 6, Issue 1, 2011, Pages

  Ng, Chai Ann ^a,b   Hunter, Mark J ^a   Perry, Matthew D ^a   Mobli, Mehdi ^c   Ke, Ying ^a   Kuchel, Philip W ^b   King, Glenn F ^c   Stock, Daniela ^a,d   Vandenberg, Jamie I ^a,b,d  

^a VICTOR CHANG CARDIAC RESEARCH INSTITUTE   (Australia)

^b UNIVERSITY OF SYDNEY   (Australia)

^c UNIVERSITY OF QUEENSLAND   (Australia)

^d UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; AMPHIPATHIC ALPHA PROTEIN; MUTANT PROTEIN; NUCLEIC ACID BINDING PROTEIN; PEPTIDES AND PROTEINS; POTASSIUM CHANNEL HERG; PROTEIN PER ARNT SIM; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENE DELETION; GENE EXPRESSION REGULATION; GENE FUNCTION; KINETICS; MUTAGENESIS; NONHUMAN; PHENOTYPE; PROTEIN DOMAIN; PROTEIN STABILITY; PROTEIN STRUCTURE; AMINO ACID SEQUENCE; CHANNEL GATING; CHEMISTRY; GENETICS; HUMAN; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN SECONDARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ETHER-A-GO-GO POTASSIUM CHANNELS; HUMANS; ION CHANNEL GATING; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, SECONDARY; SEQUENCE DELETION;

EID: 79251559440     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0016191     Document Type: Article

References (30)

1. Sanguinetti MC, Jiang C, Curran ME, Keating MT (1995) A mechanistic link between an inherited and an acquired cardiac arrhythmia: HERG encodes the IKr potassium channel. Cell 81: 299-307.
2. Vandenberg JI, Walker BD, Campbell TJ (2001) HERG K+ channels: friend and foe. Trends Pharmacol Sci 22: 240-246.
3. Curran ME, Splawski I, Timothy KW, Vincent GM, Green ED, et al. (1995) A molecular basis for cardiac arrhythmia: HERG mutations cause long QT syndrome. Cell 80: 795-803.
4. Perrin MJ, Subbiah RN, Vandenberg JI, Hill AP (2008) Human ether-a-go-go related gene (hERG) K+ channels: function and dysfunction. Prog Biophys Mol Biol 98: 137-148.
5. Trudeau MC, Warmke JW, Ganetzky B, Robertson GA (1995) HERG, a human inward rectifier in the voltage-gated potassium channel family. Science 269: 92-95.
6. Akhavan A, Atanasiu R, Noguchi T, Han W, Holder N, et al. (2005) Identification of the cyclic-nucleotide-binding domain as a conserved determinant of ion-channel cell-surface localization. J Cell Sci 118: 2803-2812.
7. Morais Cabral JH, Lee A, Cohen S, Chait B, Li M, et al. (1998) Crystal structure and functional analysis of the HERG potassium channel N terminus: a eukaryotic PAS domain. Cell 95: 649-655.
8. Ponting CP, Aravind L (1997) PAS: a multifunctional domain family comes to light. Curr Biol 7: R674-677.
9. Gustina A, Trudeau M (2009) A recombinant N-terminal domain fully restores deactivation gating in N-truncated and Proc Natl Acad Sci USA.
10. Vandenberg JI, Torres A, Campbell T, Kuchel PW (2004) The HERG K+channel: progress in understanding the molecular basis of its unusual gating kinetics. European Biophysics Journal 33: 89-97.
11. Schonherr R, Heinemann S (1996) Molecular determinants for activation and inactivation of HERG, a human inward rectifier potassium channel. Journal of Physiology.
12. Wang J, Myers CD, Robertson GA (2000) Dynamic control of deactivation gating by a soluble amino-terminal domain in HERG K(+) channels. J Gen Physiol 115: 749-758.
13. Wang J, Trudeau MC, Zappia AM, Robertson GA (1998) Regulation of deactivation by an amino terminal domain in human ether-à-go-go-related gene potassium channels. Journal of General Physiology 112: 637-647.
14. Mobli M, Hoch JC (2008) Maximum entropy spectral reconstruction of nonuniformly sampled data. Concepts Magn Reson 32A: 436-448.
15. Bartels C, Xia TH, Billeter M, Guntert P, Wuthrich K (1995) The Program Xeasy for Computer-Supported Nmr Spectral-Analysis of Biological Macromolecules. Journal of Biomolecular NMR 6: 1-10.
16. Cornilescu G, Delaglio F, Bax A (1999) Protein backbone angle restraints from searching a database for chemical shift and sequence homology. Journal of Biomolecular NMR 13: 289-302.
17. Guntert P (2004) Automated NMR structure calculation with CYANA. Methods in Molecular Biology 278: 353-378.
18. Case D, Darden T, Cheatham I, Simmerling C, Wang J, et al. (2008) AMBER 10. University of California, San Francisco.
19. Bhattacharya A, Tejero R, Montelione GT (2007) Evaluating protein structures determined by structural genomics consortia. Proteins 66: 778-795.
20. Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, et al. (2000) The Protein Data Bank. Nucleic Acids Res 28: 235-242.
21. Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. Journal of Biomolecular NMR 44: 213-223.
22. Clarke CE, Hill AP, Zhao J, Kondo M, Subbiah RN, et al. (2006) Effect of S5P alpha-helix charge mutants on inactivation of hERG K+ channels. J Physiol 573: 291-304.
23. Chen VB, Arendall WB, 3rd, Headd JJ, Keedy DA, Immormino RM, et al. (2010) MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallographica Section D: Biological Crystallography 66: 12-21.
24. Pandini A, Bonati L (2005) Conservation and specialization in PAS domain dynamics. Protein Engineering Design and Selection 18: 127-137.
25. Li Q, Gayen S, Chen AS, Huang Q, Raida M, et al. (2010) NMR solution structure of the N-terminal domain of hERG and its interaction with the S4-S5 linker. Biochem Biophys Res Commun.
26. Van Slyke AC, Rezazadeh S, Snopkowski M, Shi P, Allard CR, et al. (2010) Mutations within the S4-S5 linker alter voltage sensor constraints in hERG K+channels. Biophys J 99: 2841-2852.
27. Al-Owais M, Bracey K, Wray D (2009) Role of intracellular domains in the function of the herg potassium channel. European Biophysics Journal 38: 569-576.
28. Kolbe K, Schonherr R, Gessner G, Sahoo N, Hoshi T, et al. (2010) Cysteine 723 in the C-linker segment confers oxidative inhibition of hERG1 potassium channels. J Physiol 588: 2999-3009.
29. Muskett FW, Thouta S, Thomson SJ, Bowen A, Stansfeld PJ, et al. (2010) Mechanistic insight into hERG K+ channel deactivation gating from the solution structure of the EAG domain. J Biol Chem.
30. Moglich A, Ayers RA, Moffat K (2009) Structure and signaling mechanism of Per-ARNT-Sim domains. Structure 17: 1282-1294.