Evolutionary optimization of peptide substrates for proteases that exhibit rapid hydrolysis kinetics

Biotechnology and Bioengineering

Volumn 106, Issue 3, 2010, Pages 339-346

  Boulware, Kevin T ^a   Jabaiah, Abeer ^a   Daugherty, Patrick S ^a,b  

^a University of California   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Bacterial display; CLiPS; FACS; Peptide libraries; TEV

Indexed keywords

CLEAVAGE KINETICS; CLEAVAGE SITES; CONSENSUS MOTIF; EVOLUTIONARY OPTIMIZATIONS; HYDROLYSIS KINETICS; HYDROLYSIS RATE; LIBRARY SCREENING; MOLECULAR IMAGING; PEPTIDE LIBRARY; PEPTIDE SUBSTRATES; PRODRUGS; PROTEASE ACTIVITIES; SEQUENCE SIMILARITY; SUBSTRATE SELECTIVITY; TEV PROTEASE; TOBACCO ETCH VIRUS; TWO-COLOR; VIRAL PROTEASE;

AMINO ACIDS; BACTERIOLOGY; HYDROLYSIS; LIBRARIES; OPTIMIZATION; ORGANIC ACIDS; PEPTIDES; VIRUSES;

SUBSTRATES;

PENTAPEPTIDE; PEPTIDE LIBRARY; PROTEINASE; VIRUS ENZYME;

ARTICLE; CONSENSUS SEQUENCE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HYDROLYSIS KINETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT VIRUS; PROCESS OPTIMIZATION; PROTEIN CLEAVAGE; PROTEIN HYDROLYSIS; PROTEIN MOTIF; SCREENING; SEQUENCE HOMOLOGY; TOBACCO VIRUS;

BINDING SITES; HYDROLYSIS; KINETICS; PEPTIDE HYDROLASES; PEPTIDES; POTYVIRUS; SUBSTRATE SPECIFICITY; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); TOBACCO ETCH VIRUS;

EID: 77953570947     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22693     Document Type: Article

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