Depletion of phosphatidylcholine affects endoplasmic reticulum morphology and protein traffic at the Golgi complex

Journal of Lipid Research

Volumn 50, Issue 11, 2009, Pages 2182-2192

  Testerink, Nicole ^a   van der Sanden, Michiel H M ^a   Houweling, Martin ^a   Helms, J Bernd ^a   Vaandrager, Arie B ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

CTP; Diacylglycerol; Lipid droplets; Phosphocholine cytidylyltransferase; Protein transport

Indexed keywords

CHOLINE PHOSPHATE CYTIDYLYLTRANSFERASE; FAT DROPLET; LYSOPHOSPHATIDYLCHOLINE; PHOSPHATIDYLCHOLINE; TRIACYLGLYCEROL;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LEVEL; CELL MEMBRANE; CELL PROLIFERATION; CELL STRUCTURE; CHO CELL; ELECTRON MICROSCOPY; ENDOPLASMIC RETICULUM; FLUORESCENCE MICROSCOPY; GOLGI COMPLEX; LIPID ANALYSIS; LIPID METABOLISM; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT;

ANIMALS; CELL MEMBRANE; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE; CRICETINAE; CRICETULUS; ENDOPLASMIC RETICULUM; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; GOLGI APPARATUS; GREEN FLUORESCENT PROTEINS; LYSOPHOSPHATIDYLCHOLINES; MEMBRANE GLYCOPROTEINS; MICROSCOPY, CONFOCAL; MICROSCOPY, IMMUNOELECTRON; MUTATION; OLEIC ACID; PHOSPHATIDYLCHOLINES; PROTEIN TRANSPORT; TEMPERATURE; TRIGLYCERIDES; TRITIUM; VIRAL ENVELOPE PROTEINS;

EID: 70350418594     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.M800660-JLR200     Document Type: Article

References (44)

1. Kent, C. 1990. Regulation of phosphatidylcholine biosynthesis. Prog. Lipid Res. 29: 87-105.
2. Exton, J. H. 1994. Phosphatidylcholine breakdown and signal transduction. Biochim. Biophys. Acta. 1212: 26-42.
3. Billah, M. M., and J. C. Anthes. 1990. The regulation and cellular functions of phosphatidylcholine hydrolysis. Biochem. J. 269: 281-291.
4. Kiss, Z. 1990. Effects of phorbol ester on phospholipid metabolism. Prog. Lipid Res. 29: 141-166.
5. Nishizuka, Y. 1995. Proteine kinase C and lipid signaling for sustained cellular responses. FASEB J. 29: 484-496.
6. Jackowski, S. 1996. Cell cycle regulation of membrane phospholipid metabolism. J. Biol. Chem. 271: 20219-20222.
7. Houweling, M., Z. Cui, C. D. Abfuso, M. Bussiere, M. H. Chen, and D. E. Vance. 1996. CTP-phosphocholine cytidylyltrasferase is both a nuclear and cytoplasmic protein in primary hepatocytes. Eur J Cell Biol 69: 55-63.
8. Lykidis, A., I. Baburina, and S. Jackowski. 1999. Distribution of CTP:phosphocholine cytidylyltransferase (CCT) isoforms. Identifi cation of a new CCTbeta splice variant. J Biol Chem 274: 26992-27001.
9. Kent, C. 1997. CTP:phosphocholine cytidylyltransferase. Biochim Biophys Acta 1348: 79-90.
10. Dunne, S. J., R. B. Cornell, J. E. Johnson, N. R. Glover, and A. S. Tracey. 1996. Structure of the membrane binding domain of CTP:phosphocholine cytidylyltransferase. Biochemistry. 35: 11975-11984.
11. Cornell, R. B. 1998. How cytidylyltransferase uses an amphipathic helix to sense membrane phospholipid composition. Biochem. Soc. Trans. 26: 539-544.
12. Baburina, I., and S. Jackowski. 1998. Apoptosis triggered by 1-O-octadecyl-2-O-methyl-rac-glycero-3- phosphocholine is prevented by increased expression of CTP:phosphocholine cytidylyltransferase. J. Biol. Chem. 273: 2169-2173.
13. Esko, J. D., M. Nishijima, and C. R. Raetz. 1982. Animal cells dependent on exogenous phosphatidylcholine for membrane biogenesis. Proc. Natl. Acad. Sci. USA. 79: 1698-1702.
14. Esko, J. D., M. M. Wermuth, and C. R. Raetz. 1981. Thermolabile CDP-choline synthetase in an animal cell mutant defective in lecithin formation. J Biol Chem 256: 7388-7393.
15. Esko, J. D., and C. R. Raetz. 1980. Autoradiographic detection of animal cell membrane mutants altered in phosphatidylcholine synthesis. Proc. Natl. Acad. Sci. USA. 77: 5192-5196.
16. Cui, Z., M. Houweling, M. H. Chen, M. Record, H. Chap, D. E. Vance, and F. Terce. 1996. A genetic defect in phosphatidylcholine biosynthesis triggers apoptosis in Chinese hamster ovary cells. J. Biol. Chem. 271: 14668-14671.
17. van der Sanden, M. H. M., M. Houweling, L. M. G. van Golde, and A. B. Vaandrager. 2003. Inhibition of phosphatidylcholine synthesis induces expression of the endoplasmic reticulum stress and apoptosis related protein C/EBP-Homologous Protein (CHOP/GADD153). Biochem. J. 369: 643-650.
18. Sweitzer, T. D., and C. Kent. 1994. Expression of wild-type and mutant rat liver CTP: phosphocholine cytidylyltransferase in a cytidylyltransferase-defi cient Chinese hamster ovary cell line. Arch. Biochem. Biophys. 311: 107-116.
19. Bligh, E. G., and W. J. Dyer. 1959. A rapid method of total lipid extraction and purifi cation. Can. J. Biochem. Physiol. 37: 911-917.
20. Wieland, O. H. 1984.Glycerol UV-method. In Methods of Enzymatic Analysis. Vol. 6. H. U. Bergmeyer, editor. Verlag Chemie, Weinheim, Germany. 504-510.
21. Martin, S., and R. G. Parton. 2006. Lipid droplets: a unifi ed view of a dynamic organelle. Nat Rev Mol Cell Biol 7: 373-378.
22. Hirschberg, K., C. M. Miller, J. Ellenberg, J. F. Presley, E. D. Siggia, R. D. Phair, and J. Lippincott-Schwartz. 1998. Kinetic analysis of secretory protein traffi c and characterization of Golgi to plasma membrane transport intermediates in living cells. J. Cell Biol. 143: 1485-1503.
23. Griffiths, G., S. Pfeiffer, K. Simons, and K. Matlin. 1985. Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane. J. Cell Biol. 101: 949-964.
24. Baburina, I., and S. Jackowski. 1999. Cellular responses to excess phospholipids. J. Biol. Chem. 274: 9400-9408.
25. Barbour, S. E., A. Kapur, and C. L. Deal. 1999. Regulation of phosphatidylcholine homeostasis by calcium-independent phospholipase A2. Biochim. Biophys. Acta. 1439: 77-88.
26. Zaccheo, O., D. Dinsdale, P. A. Meacock, and P. Glynn. 2004. Neuropathy target esterase and its yeast homologue degrade phosphatidylcholine to glycerophosphocholine in living cells. J. Biol. Chem. 279: 24024-24033.
27. Martins de Lima, T., M. F. Cury-Boaventura, G. Giannocco, M. T. Nunes, and R. Curi. 2006. Comparitive toxicity of fatty acids on a macrofage cell line (J774). Clin. Sci. 111: 307-317.
28. Jackowski, S., J. Wang, and I. Baburina. 2000. Activity of the phosphatidylcholine biosynthetic pathway modulates the distribution of fatty acids into glycerolipids in proliferating cells. Biochim. Biophys. Acta. 1483: 301-315.
29. Waite, K. A., and D. E. Vance. 2000. Why expression of phosphatidylethanolamine N-methyltransferase does not rescue Chinese hamster ovary Cells that have an impaired CDP-choline pathway. J. Biol. Chem. 275: 21197-21202.
30. Guo, Y., T. C. Walther, M. Rao, N. Stuurman, G. Goshima, K. Terayama, J. S. Wong, R. D. Vale, P. Walter, and R. V. Farese, Jr. 2008. Functional genomic screen reveals genes involved in lipid droplet formation and utilisation. Nature. 453: 657-661.
31. Caviglia, J. M., I. N. De Gomez Dumm, R. A. Coleman, and R. A. Igal. 2004. Phosphatidylcholine deficiency upregulates enzymes of triacylglycerol metabolism in CHO cells. J. Lipid Res. 45: 1500-1509.
32. Bolender, R. P. 1974. Stereological analysis of the guinea pig pancreas. J. Cell Biol. 61: 269-287.
33. Dayel, M. J., E. F. Y. Hom, and A. S. Verkman. 1999. Diffusion of green fl uorescent protein in the aqueous-phase lumen of endoplasmic reticulum. Biophys. J. 76: 2843-2851.
34. Borradaile, N. M., X. Han, J. D. Harp, S. E. Gale, D. S. Ory, and J. E. Schaffer. 2006. Disruption of endoplasmic reticulum structure and integrity in lipotoxic cell death. J. Lipid Res. 47: 2726-2737.
35. Rutkowski, D. T., and R. J. Kaufman. 2004. A trip to the ER: coping with stress. Trends Cell Biol. 14: 20-28.
36. Bernales, S., S. Schuck, and P. Walter. 2007. ER-phagy: selective autophagy of the endoplasmic reticulum. Autophagy 3: 285-287.
37. van der Sanden, M. H. M., H. Meems, M. Houweling, J. B. Helms, and A. B. Vaandrager. 2004. Induction of CCAAT/enhancerbinding protein (C/ EBP)-homologous protein/growth arrest and DNA damage-inducible protein 153 expression during inhibition of phosphatidylcholine synthesis is mediated via activation of a C/EBP-activating transcription factor-responsive element. J. Biol. Chem. 279: 52007-52015.
38. Tian, Y., C. Pate, A. Andreolotti, L. Wang, E. Tuomanen, K. Boyd, E. Claro, and S. Jackowski. 2008. Cytokine secretion requires phosphatidylcholine synthesis. J. Cell Biol. 181: 945-957
39. Mousley, C. J., K. R. Tyeryar, P. Vincent-Pope, and V. A. Bankaitis. 2007. The Sec14-superfamily and the regulatory interface between phospholipid metabolism and membrane traffi cking. Biochem. Biophys. Acta. 1771: 727-736.
40. Corda, D., C. Hidalgo Carcedo, M. Bonazzi, A. Luini, and S. Spano. 2002. Molecular aspects of membrane fi ssion in the secretory pathway. Cell Mol. Life Sci. 59: 1819-1832.
41. Baron, C. L., and V. Malhotra. 2002. Role of diacylglycerol in PKD recruitment to the TGN and protein transport to the plasma membrane. Science. 295: 325-338.
42. McGee, T. P., H. B. Skinner, E. A. Whitters, S. A. Henry, and V. A. Bankaitis. 1994. A phosphatidylinositol transfer protein controls the phosphatidylcholine content of yeast Golgi membranes. J. Cell Biol. 124: 273-287.
43. Litvak, V., N. Dahan, S. Ramachandran, H. Sabanay, and S. Lev. 2005. Maintenance of the diacylglycerol level in the Golgi apparatus by the Nir2 protein is critical for Golgi secretory function. Nat. Cell Biol. 7: 225-234.
44. van der Sanden, M. H. M., M. Houweling, D. Dijsings, A. B. Vaandrager, and L. M. G. van Golde. 2004. Inhibition of phosphatidylcholine is not the primary pathway in hexadecylphosphocholine-induced apoptosis. Biochim. Biophys. Acta. 1636: 99-107.