Fractional 13C enrichment of isolated carbons using [1-13C]- or [2-13C]-glucose facilitates the accurate measurement of dynamics at backbone Cα and side-chain methyl positions in proteins

Journal of Biomolecular NMR

Volumn 38, Issue 3, 2007, Pages 199-212

  Lundström, Patrik ^a   Teilum, Kaare ^b   Carstensen, Tommy ^b   Bezsonova, Irina ^a,c   Wiesner, Silke ^c   Hansen, D Flemming ^a   Religa, Tomasz L ^d   Akke, Mikael ^b   Kay, Lewis E ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b LUND UNIVERSITY   (Sweden)

^c HOSPITAL FOR SICK CHILDREN   (Canada)

^d MRC CENTRE FOR PROTEIN ENGINEERING   (United Kingdom)

Author keywords

13C relaxation measurements; 1 13C glucose; 2 13C glucose; CPMG relaxation dispersion; Protein expression; Selective 13C labeling; T1

Indexed keywords

AMINO ACID; CARBON 13; GLUCOSE; ISOLEUCINE; PROTEIN; THREONINE; CARBON; DRUG DERIVATIVE; METHANE; METHYL RADICAL; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; DYNAMICS; MEASUREMENT; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN EXPRESSION; AMINO ACID SEQUENCE; ANIMAL; BIOSYNTHESIS; CATTLE; CHEMISTRY; HUMAN; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE;

AMINO ACID SEQUENCE; AMINO ACIDS; ANIMALS; CARBON; CARBON ISOTOPES; CATTLE; GLUCOSE; HUMANS; METHANE; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEINS;

EID: 34250903564     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-007-9158-6     Document Type: Article

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