Recombinant antimicrobial peptide hPAB-β expressed in Pichia pastoris, a potential agent active against methicillin-resistant Staphylococcus aureus

Applied Microbiology and Biotechnology

Volumn 89, Issue 2, 2011, Pages 281-291

  Chen, Zhijin ^a   Wang, Dongmei ^a   Cong, Yanguang ^a   Wang, Jing ^a   Zhu, Junmin ^a   Yang, Jie ^a   Hu, Zhen ^a   Hu, Xiaomei ^a   Tan, Yinling ^a   Hu, Fuquan ^a   Rao, Xiancai ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Antimicrobial activity; Antimicrobial peptide; hPAB ; Methicillin resistant Staphylococcus aureus (MRSA); Multidrug resistance; Pichia pastoris

Indexed keywords

ANTI-MICROBIAL ACTIVITY; ANTIMICROBIAL PEPTIDE; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MULTIDRUG RESISTANCE; PICHIA PASTORIS;

CELL CULTURE; COLUMN CHROMATOGRAPHY; DESORPTION; ELECTROPHORESIS; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE; MASS SPECTROMETRY; METHANOL; MICROFILTRATION; PARTICLE SPECTROMETERS; PEPTIDES; POLYACRYLATES; SODIUM SULFATE;

MICROORGANISMS;

ANTIBIOTIC G 418; BETA DEFENSIN 2; METHANOL; POLYPEPTIDE ANTIBIOTIC AGENT; PROTEIN HPAB BETA; PROTEINASE; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ANTIMICROBIAL ACTIVITY; BACTERIUM; BIOTRANSFORMATION; DESORPTION; ELECTROKINESIS; ENZYME ACTIVITY; FILTRATION; GENE EXPRESSION; INHIBITOR; ION CHROMATOGRAPHY; ION EXCHANGE; IONIZATION; MASS SPECTROMETRY; PEPTIDE; PLASMID; RECOMBINATION; YEAST;

ARTICLE; BACTERIUM ISOLATE; CELL TRANSFORMATION; CONTROLLED STUDY; CULTURE MEDIUM; ION EXCHANGE CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MEMBRANE FILTER; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS INFECTION; MOLECULAR CLONING; NONHUMAN; PHENOTYPE; PICHIA PASTORIS; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BASE SEQUENCE; BETA-DEFENSINS; GENE EXPRESSION; HUMANS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT PROTEINS;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; PICHIA PASTORIS; STAPHYLOCOCCUS AUREUS;

EID: 78651068827     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2864-0     Document Type: Article

References (48)

1. R Banerjee M Gretes L Basuino N Strynadka HF Chambers 2008 In vitro selection and characterization of ceftobiprole-resistant methicillin-resistant Staphylococcus aureus Antimicrob Agents Chemother 52 2089 2096 1:CAS:528:DC%2BD1cXmvVaisbY%3D 10.1128/AAC.01403-07 (Pubitemid 351758546)
2. B Berger-Bächi S Rohrer 2002 Factors influencing methicillin resistance in staphylococci Arch Microbiol 178 165 171 10.1007/s00203-002-0436-0 (Pubitemid 34985297)
3. MM Bradford 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248 254 1:CAS:528:DyaE28XksVehtrY%3D 10.1016/0003-2697(76)90527- 3
4. KMS Cabral MS Almeida AP Valente FCL Almeida E Kurtenbach 2003 Production of the active antifungal Pisum sativum defensin 1(Psd1) in Pichia pastoris: overcoming the inefficiency of the STE13 protease Protein Expr Purif 31 115 122 1:CAS:528:DC%2BD3sXntVGkt7w%3D 10.1016/S1046-5928(03)00136-0 (Pubitemid 37207649)
5. JL Cereghino JM Cregg 2000 Heterologous protein expression in the methylotrophic yeast Pichia pastoris FEMS Microbiol Rev 24 45 66 1:CAS:528:DC%2BD3cXltVClug%3D%3D 10.1111/j.1574-6976.2000.tb00532.x
6. O Cirioni A Giacometti W Kamysz C Silvestri A Riva A Della Vittoria A Abbruzzetti J Lukasiak G Scalise 2007 In vitro activities of tachyplesin III against Pseudomonas aeruginosa Peptides 28 747 751 1:CAS:528: DC%2BD2sXislGqsbs%3D 10.1016/j.peptides.2007.01.001 (Pubitemid 46349331)
7. M Dathe M Schumann T Wieprecht A Winkler K Matsuzaki O Murase M Beyermann E Krause M Bienert 1996 Peptide helicity and membrane surface charge modulate the balance of electrostatic and hydrophobic interactions with lipid bilayers and biological membranes Biochemistry 35 12612 12622 1:CAS:528: DyaK28XltlCqsrk%3D 10.1021/bi960835f (Pubitemid 26318059)
8. V Frecer B Ho JL Ding 2004 De novo design of potent antimicrobial peptides Antimicrob Agents Chemother 48 3349 3357 1:CAS:528:DC%2BD2cXnsVehsrw%3D 10.1128/AAC.48.9.3349-3357.2004 (Pubitemid 39129330)
9. C Friedrich MG Scott N Karunaratne H Yan REW Han-cock 1999 Salt-resistant alpha-helical cationic anti-microbial peptides Antimicrob Agents Chemother 43 1542 1548 1:CAS:528:DyaK1MXktlCksr0%3D
10. RE Hancock 2003 Concerns regarding resistance to self-proteins Microbiology 149 3343 3344 1:CAS:528:DC%2BD3sXhtVWnsL7I 10.1099/mic.0.C0122-0
11. RE Hancock DS Chapple 1999 Peptide antibiotics Antimicrob Agents Chemother 43 1317 1323 1:CAS:528:DyaK1MXjs12nsrc%3D
12. J Harder J Bartels E Christophers JM Schroder 1997 A peptide antibiotic from human skin Nature 387 861 1:CAS:528:DyaK2sXkt1Gmtbo%3D 10.1038/43088 (Pubitemid 27289577)
13. C Haught GD Davis R Subramanian KW Jackson RG Harrison 1998 Recombinant production and purification of novel anti-sense antimicrobial peptide in Escherichia coli Biotechnol Bioeng 57 55 61 1:CAS:528:DyaK2sXnvVGgtL0%3D 10.1002/(SICI)1097-0290(19980105)57:1<55::AID-BIT7>3.0.CO;2-U (Pubitemid 27514700)
14. L Huang JF Wang ZX Zhong L Peng HQ Chen ZN Xu PL Cen 2006 Production of bioactive human beta-defensin-3 in Escherichia coli by soluble fusion expression Biotechnol Lett 28 627 632 1:CAS:528:DC%2BD28XjvFSnuro%3D 10.1007/s10529-006- 0024-5
15. L Huang SS Leong R Jiang 2009 Soluble fusion expression and characterization of bioactive human beta-defensin 26 and 27 Appl Microbiol Biotechnol 84 301 308 1:CAS:528:DC%2BD1MXpsVarsbw%3D 10.1007/s00253-009-1982-z
16. F Jin X Xu W Zhang D Gu 2006 Expression and characterization of a housefly cecropin gene in the methylotrophic yeast, Pichia pastoris Protein Expr Purif 49 39 46 1:CAS:528:DC%2BD28XovVemsrw%3D 10.1016/j.pep.2006.03.008 (Pubitemid 44278630)
17. SJ Kim R Quan SJ Lee HK Lee JK Choi 2009 Antibacterial activity of recombinant hCAP18/LL37 protein secreted from Pichia pastoris J Microbiol 47 358 362 1:CAS:528:DC%2BD1MXnslemsrg%3D 10.1007/s12275-009-0131-9
18. M Kuroda T Ohta I Uchiyama T Baba H Yuzawa, et al. 2001 Whole genome sequencing of meticillin-resistant Staphylococcus aureus Lancet 357 1225 1240 1:CAS:528:DC%2BD3MXjvVClur0%3D 10.1016/S0140-6736(00)04403-2 (Pubitemid 32539050)
19. AS Ladokhin SH White 2001 "Detergent-like" permeabilization of anionic lipid vesicles by melittin Biochim Biophys Acta 1514 253 260 1:CAS:528:DC%2BD3MXms1OlsLk%3D 10.1016/S0005-2736(01)00382-0 (Pubitemid 32831013)
20. AS Ladokhin ME Selsted SH White 1997 Sizing membrane pores in lipid vesicles by leakage of co-encapsulated markers: pore formation by melittin Biophys J 72 1762 1766 1:CAS:528:DyaK2sXitFeit7s%3D 10.1016/S0006-3495(97)78822- 2
21. JH Lee I Minn CB Park SC Kim 1998 Acidic peptide-mediated expression of the antimicrobial peptide buforin II as tandem repeats in Escherichia coli Protein Expr Purif 12 53 60 1:CAS:528:DyaK1cXht1yis7g%3D 10.1006/prep.1997.0814 (Pubitemid 28361969)
22. JH Lee JH Kim SW Hwang WJ Lee HK Yoon HS Lee SS Hong 2000 High- level expression of antimicrobial peptide mediated by a fusion partner reinforcing formation of inclusion bodies Biochem Biophys Res Commun 277 575 580 1:CAS:528:DC%2BD3cXnslCqurs%3D 10.1006/bbrc.2000.3712
23. L Li JX Wang XF Zhao CJ Kang N Liu JH Xiang FH Li S Sueda H Kondo 2005 High level expression, purification, and characterization of the shrimp antimicrobial peptide, Ch-penaeidin, in Pichia pastoris Protein Expr Purif 39 144 151 1:CAS:528:DC%2BD2MXitFalsg%3D%3D 10.1016/j.pep.2004.09.006 (Pubitemid 40084599)
24. S Macauley-Patrick ML Fazenda B McNeil LM Harvey 2005 Heterologous protein production using the Pichia pastoris expression system Yeast 22 249 270 1:CAS:528:DC%2BD2MXjsVals7k%3D 10.1002/yea.1208 (Pubitemid 40528349)
25. WL Maloy UP Kari 1995 Structure-activity studies on magainins and other host defense peptides Biopolymers 37 105 122 1:CAS:528:DyaK2MXjsV2ju70%3D 10.1002/bip.360370206
26. K Matsuzaki 1998 Magainins as paradigm for the mode of action of pore forming polypeptides Biochim Biophys Acta 1376 391 400 1:CAS:528: DyaK1cXntVyit70%3D
27. C Monica T Damaso MS Almeida E Kurtenbach OB Martins N Pereira JMMC Andrade RM Albano 2003 Optimized expression of a thermostable xylanase from Thermomyces lanuginosus in Pichia pastoris Appl Enviro Microbiol 69 6064 6072 10.1128/AEM.69.10.6064-6072.2003 (Pubitemid 37287580)
28. L Peng ZN Xu XM Fang F Wang S Yang PL Cen 2004 Preferential codons enhancing the expression level of human beta-defensin-2 in recombinant Escherichia coli Protein Pept Lett 11 339 344 1:CAS:528:DC%2BD2cXlslKit74%3D 10.2174/0929866043406760 (Pubitemid 38908580)
29. VC Pereira A Martins LMSS Rugolo MLRS Cunha 2009 Detection of oxacillin resistance in Staphylococcus aureus isolated from the neonatal and pediatric units of a Brazilian teaching hospital Clin Med Pediatr 3 23 31
30. JC Pierce WL Maloy L Salvador CF Dungan 1997 Recombinant expression of the antimicrobial peptide polyphemusin and its activity against the protozoan oyster pathogen Perkinsus marinus Mol Mar Biol Biotechnol 6 248 259 1:CAS:528:DyaK2sXlvFSjsb8%3D
31. KL Piers MH Brown REW Hancock 1993 Recombinant-DNA procedures for producing small antimicrobial cationic peptides in bacteria Gene 134 7 13 1:CAS:528:DyaK2cXisVGgsA%3D%3D 10.1016/0378-1119(93)90168-3
32. BA Plantz J Sinha L Villarete KW Nickerson VL Schlegel 2006 Pichia pastoris fermentation optimization: energy state and testing a growth-associated model Appl Microbiol Biotechnol 72 297 305 1:CAS:528:DC%2BD28XotlCiu78%3D 10.1007/s00253-005-0271-8 (Pubitemid 44273052)
33. XC Rao S Li JC Hu XL Jin XM Hu JJ Huang ZJ Chen JM Zhu FQ Hu 2004 A novel carrier molecule for high-level expression of peptide antibiotics in Escherichia coli Protein Expr Purif 36 11 18 1:CAS:528:DC%2BD2cXks1ersr8%3D 10.1016/j.pep.2004.01.020 (Pubitemid 38836759)
34. X Rao J Hu S Li X Jin C Zhang Y Cong X Hu Y Tan J Huang Z Chen J Zhu F Hu 2005 Design and expression of peptide antibiotic hPAB-β as tandem multimers in E. coli Peptides 26 721 729 1:CAS:528:DC%2BD2MXjt1Kjsr4%3D 10.1016/j.peptides.2004.12.016 (Pubitemid 40461652)
35. A Ramamoorthy S Thennarasu DK Lee A Tan L Maloy 2006 Solid-state NMR investigation of the membrane-disrupting mechanism of antimicrobial peptides MSI-78 and MSI-594 derived from magainin 2 and melittin Biophysical J 91 206 216 1:CAS:528:DC%2BD28Xms1Crsb8%3D 10.1529/biophysj.105.073890 (Pubitemid 44015320)
36. JM Reichhart M Meister JL Dimarcq D Zachary D Hoffmann C Ruiz G Richards JA Hoffmann 1992 Insect immunity: developmental and inducible activity of the Drosophila diptericin promoter EMBO J 11 1469 1477 1:CAS:528:DyaK3sXkvFent7Y%3D
37. HS Sader AA Watters TR Fritsche RN Jones 2007 Daptomycin antimicrobial activity tested against methicillin-resistant staphylococci and vancomycin-resistant enterococci isolated in European medical centers BMC Infect Dis 7 29 10.1186/1471-2334-7-29 (Pubitemid 46745435)
38. H Schaegger GV Jagow 1987 Tricine-sodium dodecyl sulfate-poly-acrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa Anal Biochem 166 368 379 1:CAS:528:DyaL1cXhvVOhsQ%3D%3D 10.1016/0003- 2697(87)90587-2 (Pubitemid 18004907)
39. U Schwab P Gilligan J Jaynes D Henke 1999 In vitro activities of designed antimicrobial peptides against multidrug-resistant cystic fibrosis pathogens Antimicrob Agents Chemother 43 1435 1440 1:CAS:528:DyaK1MXjs12nsb4%3D
40. PD Stapleton PW Taylor 2002 Methicillin resistance in Staphylococcus aureus: mechanisms and modulation Sci Prog 85 57 72 1:CAS:528: DC%2BD38XjtFOnsr4%3D 10.3184/003685002783238870
41. S Ueno K Kusaka Y Tamada M Minaba H Zhang PC Wang Y Kato 2008 Anionic C-terminal proregion of nematode antimicrobial peptide cecropin P4 precursor inhibits antimicrobial activity of the mature peptide Biosci Biotechnol Biochem 72 3281 3284 1:CAS:528:DC%2BD1MXis1emtg%3D%3D 10.1271/bbb.80397
42. C Vidaillac MJ Rybak 2009 Ceftobiprole: first cephalosporin with activity against methicillin-resistant Staphylococcus aureus Pharmacotherapy 29 511 525 1:CAS:528:DC%2BD1MXhtVGisbfE 10.1592/phco.29.5.511
43. A Wang S Wang M Shen F Chen Z Zou X Ran T Cheng Y Su J Wang 2009 High level expression and purification of bioactive human alpha-defensin 5 mature peptide in Pichia pastoris Appl Microbiol Biotechnol 84 877 884 1:CAS:528:DC%2BD1MXhtFaqsLvN 10.1007/s00253-009-2020-x
44. Z Xu L Peng Z Zhong X Fang P Cen 2006 High-level expression of a soluble functional antimicrobial peptide, human beta-defensin 2, in Escherichia coli Biotechnol Prog 22 382 386 1:CAS:528:DC%2BD28XksV2qtQ%3D%3D 10.1021/bp0502680
45. M Zasloff 2002 Antimicrobial peptides of multicellular organisms Nature 415 389 395 1:CAS:528:DC%2BD38XhtVCgsLs%3D 10.1038/415389a (Pubitemid 34100944)
46. H Zhang QP Yuan 2007 Cloning and secretion expression of hepcidin in Pichia pastoris Sheng Wu Gong Cheng Xue Bao 23 381 385 1:CAS:528: DC%2BD2sXhtFOns7vL
47. Z Zhong Z Xu L Peng L Huang X Fang P Cen 2006 Tandem repeat mhBD2 gene enhance the soluble fusion expression of hBD2 in Escherichia coli Appl Microbiol Biotechnol 71 661 667 1:CAS:528:DC%2BD28Xnt1aju7o%3D 10.1007/s00253-005-0212-6 (Pubitemid 44114794)
48. WL Zhu H Lan IS Park JI Kim HZ Jin KS Hahm SY Shin 2006 Design and mechanism of action of a novel bacteria-selective antimicrobial peptide from the cell-penetrating peptide Pep-1 Biochem Biophys Res Commun 349 769 774 1:CAS:528:DC%2BD28Xps1Ogtrg%3D 10.1016/j.bbrc.2006.08.094 (Pubitemid 44344580)