Antibacterial activity of recombinant hCAP18/LL37 protein secreted from Pichia pastoris

Journal of Microbiology

Volumn 47, Issue 3, 2009, Pages 358-362

  Kim, Soon ja ^a   Quan, Renshu ^a   Lee, Sung Jin ^b   Lee, Hak Kyo ^c   Choi, Joong Kook ^a  

^a Chungbuk National University College of Medicine   (South Korea)

^b Kangwon National University   (South Korea)

^c Hankyong National University   (South Korea)

Author keywords

Antibacterial activity; Antimicrobial peptide (AMP); hCAP18 LL37; Pichia pastoris

Indexed keywords

BACTERIA (MICROORGANISMS); MICROCOCCUS LUTEUS; PICHIA PASTORIS; SALMONELLA; STAPHYLOCOCCUS AUREUS;

ANTIINFECTIVE AGENT; ANTIMICROBIAL CATIONIC PEPTIDE; CATHELICIDIN ANTIMICROBIAL PEPTIDE; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; CULTURE MEDIUM; DRUG EFFECT; GENETICS; HUMAN; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROCOCCUS LUTEUS; PICHIA; SALMONELLA; STAPHYLOCOCCUS AUREUS;

ANTI-BACTERIAL AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; CULTURE MEDIA; HUMANS; MICROBIAL SENSITIVITY TESTS; MICROCOCCUS LUTEUS; PICHIA; RECOMBINANT PROTEINS; SALMONELLA; STAPHYLOCOCCUS AUREUS;

EID: 68349084675     PISSN: 12258873     EISSN: 12258873     Source Type: Journal    
DOI: 10.1007/s12275-009-0131-9     Document Type: Article

References (30)

1. Agerberth, B., J. Charo, J. Werr, B. Olsson, F. Idali, L. Lindbom, R. Kiessling, H. Jörnvall, H. Wigzell, and G.H. Gudmundsson. 2000. The human antimicrobial and chemotatic peptides LL37 and alpha-defensins are expressed by specific lymphocyte and monocyte populations. Blood 96, 3086-3093.
2. auley-Patrick, S., M.L. Fazenda, B. McNeil, and L.M. Harvey. 2005. Heterologous protein production using the Pichia pastoris expression system. Yeast 22, 249-270.
3. Bals, R. 2000. Epithelial antimicrobial peptides in host defense against infection. Respir. Res. 1, 141-150.
4. Bals, R., D.J. Weiner, A.D. Moscioni, R.L. Meegalla, and J.M. Wilson. 1999. Augmentation of innate host defense by expression of a cathelicidin antimicrobial peptide. Infect. Immun. 67, 6084-6089.
5. Brierley, R.A., G.R. Davis, and G.C. Holtz. 1994. Production of insulin-like growth factor in methylotrophic yeast cell. US Patent 5324639.
6. Cereghino, J.L. and J.M. Cregg. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24, 45-66.
7. Clare, J.J., M.A. Romanos, F.B. Rayment, J.E. Rowedder, M.A. Smith, M.M. Payne, K. Sreekrishna, and C.A. Henwood. 1991. Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies. Gene 105, 205-212.
8. Colilla, F.J., A. Rocher, and E. Mendez. 1990. Gamma-purothionins: amino acid sequence of two polypeptides of a new family of thionins from wheat endosperm. FEBS Lett. 270, 191-194.
9. Cowland, J.B., A.H. Johnsen, and N. Borregaard. 1995. hCAP-18, a cathelin/pro-bactenecin-like protein of human neutrophil specific granules. FEBS Lett. 368, 173-176.
10. Frohm, M., B. Agerberth, G. Ahangari, M. Stahle-Backdahl, S. Liden, H. Wiqzell, and G.H. Gudmundsson. 1997. The expression of the gene coding for the antibacterial peptide LL-37 is induced in human keratinocytes during inflammatory disorders. J. Biol. Chem. 272, 15258-15263.
11. Gudmundsson, G.H., B. Agerberth, J. Odeberg, T. Bergman, B. Olsson, and R. Salcedo. 1996. The human gene FALL39 and processing of the cathelin precursor to the antibacterial peptide LL-37 in granulocytes. Eur. J. Biochem. 238, 325-332.
12. Hancock, R.E. and M.G. Scott. 2000. The role of antimicrobial peptides in animal defenses. Proc. Natl. Acad. Sci. USA 97, 8856-8861.
13. Henzler-Wildman, K.A., D. Lee, and A. Ramamoorthy. 2003. Mechanism of lipid bilayer disruption by the human antimicrobial peptide, LL37. Biochemistry 42, 6545-6558.
14. Henzler-Wildman, K.A., G.V. Martinez, M.F. Brown, and A. Ramamoorthy. 2004. Perturbation of the hydrophobic core of lipid bilayers by the human antimicrobial peptide, LL37. Biochemistry 43, 8459-8469.
15. Iwanaga, S., S. Kawabata, and T. Muta. 1998. New types of clotting factors and defense molecules found in horseshoe crab hemolymph: their structures and functions. J. Biochem. 123, 1-15.
16. Liu, W. and J.N. Hansen. 1990. Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lactis. Appl. Environ. Microbiol. 56, 2551-2558.
17. Luders, T., G.A. Birkemo, G. Fimland, J. Nissen-Meyer, and I.F. Nes. 2003. Strong synergy between a eukaryotic antimicrobial peptide and bacteriocins from lactic acid bacteria. Appl. Environ. Microbiol. 69, 1797-1799.
18. Mendez, E., A. Moreno, F. Colilla, F. Pelaez, G.G. Limas, R. Mendez, F. Soriano, M. Salinas, and C. De Haro. 1990. Primary structure and inhibition of protein synthesis in eukaryotic cell-free system of a novel thionin, gamma-hordothionin, from barley endosperm. Eur. J. Biochem. 194, 533-539.
19. Moon, J., K.A. Henzler-Wildman, and A. Ramamoorthy. 2006. Expression and purification of a recombinant LL37 from Escherichia coli. Biochim. Biophys. Acta. 1758, 1351-1358.
20. Nakamura, T., H. Furunaka, T. Miyata, F. Tokunaga, T. Muta, S. Iwanaga, M. Niwa, T. Takao, and Y. Shimonishi. 1988. Tachyplesin, a class of antimicrobial peptide from the hemocytes of the horseshoe crab (Tachypleus tridentatus) isolation and chemical structure. J. Biol. Chem. 263, 16709-16713.
21. Reddy, K.V., R.D. Yedery, and C. Aranha. 2004. Antimicrobial peptides: premises and promises. Int. J. Antimicrob. Agent 24, 536-547.
22. Schnapp, D., C.J. Reid, and A. Harris. 1998. Localization of expression of human beta defensin-1 in the pancreas and kidney. J. Pathol. 186, 99-103.
23. Selsted, M.E., Y.Q. Tang, W.L. Morris, P.A. McGuire, M.J. Novotny, W. Smith, A.H. Henschen, and J.S. Cullor. 1993. Purification, primary structures, and antibacterial activities of beta-defensins, a new family of antimicrobial peptides from bovine neutrophils. J. Biol. Chem. 268, 6641-6648.
24. Sorensen, O.E., P. Follin, A.H. Johnsen, J. Calafat, G.S. Tjabringa, P.S. Hiemstra, and N. Borreqaard. 2001. Human cathelicidin, hCAP-18, is processed to the antimicrobial peptide LL-37 by extracellular cleavage with proteinase 3. Blood 97, 3951-3959.
25. Steiner, H., D. Hultmark, A. Engstrom, H. Bennich, and H.G. Boman. 1981. Sequence and specificity of two antibacterial proteins involved in insect immunity. Nature 292, 246-248.
26. Thevissen, K., K.K. Ferket, I.E. Francois, and B.P. Cammue. 2003. Interactions of antifungal plant defensins with fungal membrane components. Peptide 24, 1705-1712.
27. Turner, J., Y. Cho, N.N. Dinh, A.J. Waring, and R.I. Lehrer. 1998. Activities of LL-37, a cathelin-associated antimicrobial peptide of human neutrophils. Antimicrob. Agents Chemother. 42, 2206-2214.
28. Yang, Y., G. Zheng, G. Li, X. Zhang, Z. Cao, Q. Rao, and K. Wu. 2004. Expression of bioactive recombinant GSLL-39, a variant of human antimicrobial peptide LL37, in Esherichia coli. Protein Expr. Purif. 37, 229-235.
29. Zanetti, M. 2004. Cathelicidins, multifunctional peptides of the innate immunity. J. Leukoc. Biol. 75, 39-48.
30. Zasloff, M. 1987. Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor. Proc. Natl. Acad. Sci. USA 84, 5449-5453.