High level expression and purification of bioactive human α-defensin 5 mature peptide in Pichia pastoris

Applied Microbiology and Biotechnology

Volumn 84, Issue 5, 2009, Pages 877-884

  Wang, Aiping ^a   Wang, Song ^a   Shen, Mingqiang ^a   Chen, Fang ^a   Zou, Zhongmin ^a   Ran, Xinze ^a   Cheng, Tianmin ^a   Su, Yongping ^a   Wang, Junping ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Bioactivity; Expression; Human defensin 5; Pichia pastoris; Purification

Indexed keywords

ADAPTIVE IMMUNITY; ANTI-BACTERIAL ACTIVITY; ANTIMICROBIAL PEPTIDE; BIOENGINEERING TECHNIQUES; CATION EXCHANGE CHROMATOGRAPHY; CELL DENSITY; CULTURE CONDITIONS; DEFENSINS; DNA FRAGMENT; EXPRESSION; EXPRESSION SYSTEM; EXPRESSION VECTORS; GENOMIC INTEGRATION; HIGH LEVEL EXPRESSION; HOMOLOGOUS RECOMBINATION; HUMAN PAPILLOMAVIRUS; IN-VITRO; MACROPOROUS RESIN ADSORPTION; MASS SPECTROMETRY ANALYSIS; N-TERMINAL AMINO ACID SEQUENCE; N-TERMINALS; NUCLEOTIDE SEQUENCES; P. PASTORIS; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; REPRODUCTIVE TISSUES; SMALL INTESTINE;

ADSORPTION; AMINATION; AMINES; AMINO ACIDS; CELL CULTURE; CELL MEMBRANES; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; FERMENTATION; MASS SPECTROMETRY; NUCLEIC ACIDS; ORGANIC ACIDS; PEPTIDES; PLANTS (BOTANY); RESINS;

PURIFICATION;

ALPHA DEFENSIN; DNA FRAGMENT; HUMAN ALPHA DEFENSIN 5; UNCLASSIFIED DRUG;

AMINO ACID; BIOACTIVITY; FUNGUS; GENE EXPRESSION; IMMUNITY; PEPTIDE;

ANTIBACTERIAL ACTIVITY; ANTIVIRAL ACTIVITY; ARTICLE; CELL DENSITY; CONTROLLED STUDY; DRUG IDENTIFICATION; DRUG PURIFICATION; FERMENTATION MEDIUM; FUNGAL CELL; FUNGAL STRAIN; HUMAN; HUMAN CELL; IN VITRO STUDY; MASS SPECTROMETRY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; VIRUS INFECTION; WESTERN BLOTTING;

ALPHA-DEFENSINS; AMINO ACID SEQUENCE; ANTI-INFECTIVE AGENTS; BASE SEQUENCE; CELL LINE; CLONING, MOLECULAR; GENE EXPRESSION; GENETIC ENGINEERING; HUMANS; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT FUSION PROTEINS;

HUMAN PAPILLOMAVIRUS; PICHIA PASTORIS;

EID: 70349263689     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2020-x     Document Type: Article

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