Purification and in situ immobilization of papain with aqueous two-phase system

PLoS ONE

Volumn 5, Issue 12, 2010, Pages

  Li, Mingliang ^a   Su, Erzheng ^a   You, Pengyong ^a   Gong, Xiangyu ^a   Sun, Ming ^a   Xu, Diansheng ^a   Wei, Dongzhi ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CHITOSAN; CROSS LINKING REAGENT; LATEX; MACROGOL 6000; PAPAIN; ENZYME; IMMOBILIZED ENZYME; MACROGOL DERIVATIVE; WATER;

AMINATION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; HYDROLYSIS; NONHUMAN; PAPAYA; PH; PROCESS OPTIMIZATION; RESPONSE SURFACE METHOD; BIOCHEMISTRY; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE;

CARICA PAPAYA;

BIOCHEMISTRY; CARICA; CHITOSAN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CROSS-LINKING REAGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYMES; ENZYMES, IMMOBILIZED; HYDROGEN-ION CONCENTRATION; LATEX; PAPAIN; POLYETHYLENE GLYCOLS; TEMPERATURE; WATER;

EID: 78650788079     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0015168     Document Type: Article

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