Large-scale separation and production of engineered proteins, designed for facilitated recovery in detergent-based aqueous two-phase extraction systems

Process Biochemistry

Volumn 39, Issue 7, 2004, Pages 889-896

  Selber, Klaus ^a   Tjerneld, Folke ^b   Collén, Anna ^b   Hyytiä, Teppo ^c   Nakari Setälä, Tiina ^c   Bailey, Michael ^c   Fagerström, Richard ^c   Kan, John ^d   Van Der Laan, Joop ^d   Penttilä, Merja ^c   Kula, Maria Regina ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b LUND UNIVERSITY   (Sweden)

^c VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^d Genencor International BV   (Netherlands)

Author keywords

Detergent based aqueous two phase systems; Endoglucanase I; Hydrophobin fusion protein; Large scale extraction

Indexed keywords

HYPOCREA JECORINA; TRICHODERMA;

EID: 1542653049     PISSN: 00329592     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0032-9592(03)00198-5     Document Type: Article

References (27)

1. Walter H., Brooks D.E., Fisher D. Partitioning in Aqueous Two-Phase Systems. 1985;Academic Press, Orlando, FL.
2. Albertsson P.A. Partition of Cell Particles and Macromolecules. 1986;Wiley, New York.
3. Kula M.-R., Selber K. Protein Purification, Aqueous Liquid Extraction. Flickinger M.C., Drew S.W. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation. 1999;Wiley, New York.
4. Kroner K.H., Hustedt H., Kula M.-R. Process Biochem. 19:1984;170-179.
5. Kula M.-R. Trends and future aspects of aqueous two-phase extraction. Bioseparation. 1:1990;181-189.
6. Sánchez-Ferrer Á., Bru R., García-Carmona F. Phase separation of biomolecules in polyoxyethylene glycol nonionic detergents. Crit. Rev. Biochem. Mol. Biol. 29:(4):1994;275-313.
7. Hinze W.L., Pramauro E. A critical review of surfactant-mediated phase separation (cloud-point extractions): theory and application. Crit. Rev. Anal. Chem. 24:(2):1993;133-177.
8. Kroner K.H., Schütte H., Stach W., Kula M.-R. Scale up of formate dehydrogenase by partition. J. Chem. Technol. Biotechnol. 32:1982;130-137.
9. Kim CY, Brewer JW, Brothers CE, Farver TF, Lee EK. Paper presented at the 3rd Chemical Congress of North America, Toronto, June 5-10, 1988.
10. Heinsohn HG, Lorch JD, Arnold RE. US Patent 5,139,943, 1992.
11. Minuth T., Gieren H., Pape U., Raths H.C., Thömmes J., Kula M.-R. Processing of detergent based aqueous two-phase systems in the pilot scale. Biotechnol. Bioeng. 55:1997;339-347.
12. Selber K., Müller S., Gieren H., Thömmes J., Sottmann T., Strey R., Kula M.-R. Physical properties of detergent based aqueous two-phase systems. Bioseparation. 10:2002;243-254.
13. Berggren K., Veide A., Nygren P.-A., Tjerneld F. Genetic engineering of protein-peptide fusions for control of protein partitioning in thermoseparating aqueous two-phase systems. Bio/Bio. 62:1999;135-144.
14. Collén A., Ward M., Tjerneld F., Stålbrand H. Genetic engineering of the Trichoderma reesei endoglucanase I (Cel 7B) for enhanced partitioning in aqueous two-phase systems containing thermoseparating ethyleneoxide-propyleneoxide copolymers. J. Biotechnol. 87:2001;179-191.
15. Collén A, Selber K, Hyytiä T, Persson P, Nakari- Setälä T, Bailey M, Fagerström R, Kula M-R, Penttilä M, Stålbrand H, Tjerneld F. Primary recovery of a genetically engineered Trichoderma reesei endoglucanase I (Cel 7B) fusion protein in cloud-point extraction systems, Biotechonol Bioeng 2002;78:385-394.
16. Minuth T., Thömmes J., Kula M.-R. A closed concept for the purification of the membrane-bound cholesterol oxidase from nochordia rhodochrous by surfactant-based cloud-point extraction, organic solvent extraction and anion-exchange chromatography. Biotechnol. Appl. Biochem. 23:1996;107-116.
17. Fauquex P. F., Hustedt H., Kula M.-R. Phase equilibration in agitated vessels during extractive enzyme recovery. J. Chem. Tech. Biotechnol. 35B:1985;51-59.
18. Mandels M., Weber J. The production of cellulases. Adv. Chem. Ser. 95:1969;391-414.
19. Selber K., Collén A., Hyytiä T., Penttilä M., Tjerneld F., Kula M.-R. Parameters influencing extraction in detergent based aqueous two-phase systems. Bioseparation. 10:2002;229-236.
20. Selber K. Primary Recovery of Genetically Modified Proteins Using Detergent Based Aqueous Two-Phase Extraction. 2000;Shaker Verlag Aachen, Germany.
21. Kroner K.H., Hustedt H., Kula M.-R. Evaluation of crude dextran as phase forming polymer for the extraction of enzymes in aqueous two-phase systems in large scale. Biotechnol. Bioeng. 24:1982;1015-1045.
22. Strey R. Water-nonionic surfactant systems, and the effect of additives. Ber. Bunsen-Ges. Phys. Chem. 100:1996;182-189.
23. Wormuth K.R. Patterns of phase behaviour in polymer and amphiphile mixtures. Langmuir. 7:1991;1622-1626.
24. Pryde J.G., Philipps J.H. Fractionation of membrane proteins by temperature induced phase separation in Triton X-114 based aqueous two-phase systems. J. Biochem. 233:1986;525-533.
25. Terstappen G.C., Geerts A.J., Kula M.-R. The use of detergent based aqueous two-phase systems for the isolation of extracellular proteins: purification of a lipase from Pseudomonas cepacia. Biotechnol. Appl. Biochem. 16:1992;228-235.
26. Tomme P., van Tilbeurgh H., Petterson G., Damme J., van Vandekerckhove J., Knowles J., Teeri T., Caeyssens M. Studies of the cellulytic system of Trichoderma reesei QM9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis. Eur. J. Biochem. 170:1988;575-581.
27. Johansson G., Ståhlberg G., Lindeberg G., Engström Å., Pettersson G. Isolated fungal cellulase terminal domains and a synthetic minimum analogue bind to cellulose. FEBS Lett. 243:1989;389-393.