Purification and in situ immobilization of lipase from of a mutant of Trichosporon laibacchii using aqueous two-phase systems

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 878, Issue 11-12, 2010, Pages 909-912

  Zhang, Yuan yuan ^a   Liu, Jun hong ^a  

^a QINGDAO UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Aqueous two phase system; Immobilization; Lipase; Purification; Trichosporon laibacchii

Indexed keywords

ACTIVITY RECOVERY; AQUEOUS TWO PHASE SYSTEM; CELL HOMOGENATE; IMMOBILIZED LIPASE; IN-SITU; IS COSTS; LIPASE IMMOBILIZATION; LIPASE PURIFICATION; PARTIAL PURIFICATION; PARTITION COEFFICIENT; POTASSIUM PHOSPHATE; PURIFICATION FACTORS; RICH PHASE; TRICHOSPORON;

ENZYME IMMOBILIZATION; PHOSPHATES; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; POTASSIUM; PURIFICATION; SODIUM CHLORIDE;

CELL IMMOBILIZATION;

INFUSORIAL EARTH; MACROGOL; POTASSIUM DIHYDROGEN PHOSPHATE; TRIACYLGLYCEROL LIPASE;

AQUEOUS SOLUTION; ARTICLE; COST EFFECTIVENESS ANALYSIS; ENZYME ACTIVITY; FUNGUS MUTANT; NONHUMAN; PH; PHASE PARTITIONING; PHASE SEPARATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PURIFICATION; TRICHOSPORON; TRICHOSPORON LAIBACCHII;

ENZYMES, IMMOBILIZED; HYDROGEN-ION CONCENTRATION; LIPASE; MOLECULAR WEIGHT; MUTATION; PHASE TRANSITION; POLYETHYLENE GLYCOLS; TRICHOSPORON; WATER;

EID: 77949490955     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2010.01.045     Document Type: Article

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