Ultrastructural alterations induced by δ24(25)-sterol methyltransferase inhibitors on Trichomonas vaginalis

FEMS Microbiology Letters

Volumn 315, Issue 1, 2011, Pages 72-78

  De Andrade Rosa, Ivone ^a,b   Rocha, Débora Afonso Silva ^a   De Souza, Wanderley ^b   Urbina, Julio A ^c   Benchimol, Marlene ^a  

^a UNIVERSIDADE SANTA ÚRSULA   (Brazil)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^c INSTITUTO VENEZOLANO DE INVESTIGACIONES CIENTÍFICAS   (Venezuela)

Author keywords

Cell death; Chemotherapeutic compounds; Membrane alterations; Sterol biosynthesis inhibitors; Trichomonas vaginalis; Ultrastructure

Indexed keywords

22,26 AZASTEROL; 24,25 EPIMINOLANOSTEROL; METHYLTRANSFERASE INHIBITOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIPROTOZOAL ACTIVITY; ARTICLE; AUTOPHAGY; CELL CULTURE; CELL CYCLE ARREST; CELL DAMAGE; CELL ENLARGEMENT; CELL PROLIFERATION; CELL ULTRASTRUCTURE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY TEST; DRUG SELECTIVITY; GOLGI COMPLEX; IN VITRO STUDY; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; TRICHOMONAS VAGINALIS; TROPHOZOITE;

ANIMALS; ANTITRICHOMONAL AGENTS; CELL LINE; CELL PROLIFERATION; CHOLESTANOL; DOGS; ENZYME INHIBITORS; LANOSTEROL; METHYLTRANSFERASES; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; PARASITIC SENSITIVITY TESTS; TOXICITY TESTS; TRICHOMONAS VAGINALIS;

MAMMALIA; TRICHOMONAS VAGINALIS;

EID: 78650786413     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02178.x     Document Type: Article

References (33)

1. Atencio R, Visbal G, Pekerar S, Papale J & Urbina JA (2001) Studies of molecular structure parameters of 20-piperidin-2-yl-5alpha-pregnan-3beta, 20-diol and its N-methyl derivative: two inhibitors of Delta24(25) sterol methyl transferase and Delta24(24') sterol methyl reductase of Trypanosoma cruzi. Acta Crystallogr B 57: 714-721.
2. Benchimol M (2008) The hydrogenosome as a drug target. Curr Pharm Design 14: 872-881.
3. Contreras LM, Vivas J & Urbina JA (1997) Altered lipid composition and enzyme activities of plasma membranes from Trypanosoma (Schizotrypanum) cruzi epimastigotes grown in the presence of sterol biosynthesis inhibitors. Pharmacology 53: 697-704.
4. Dantas-Leite L, Urbina JA, De Souza W & Vommaro RC (2005) Antiproliferative synergism of azasterols and antifolates against Toxoplasma gondii. Int J Antimicrob Ag 25: 130-135.
5. Diamond LS (1957) The establishment of various trichomonads of animalis and man in axenic cultures. J Parasitol 43: 488-490.
6. Dulbecco R & Freeman G (1959) Plaque production by the polyoma virus. Virology 8: 396-397.
7. Dunne R, Dunn L, Upcroft P, O'Donoghue P & Upcroft J (2003) Drug resistance in the sexually transmitted protozoan Trichomonas vaginalis. Cell Res 13: 239-249.
8. Durel P, Couture J, Collart P & Girot C (1960) Flagyl (metronidazole). Brit J Vener Dis 36: 154-162.
9. Edinger AL & Thompson CB (2004) Death by design: apoptosis, necrosis and autophagy. Curr Opin Cell Biol 16: 663-669.
10. Goldman LM, Upcroft JA, Workowski K & Rapkin A (2009) Treatment of metronidazole-resistant Trichomonas vaginalis. Sex Health 6: 345-347.
11. Gros L, Castillo-Acosta VM, Jiménez Jiménez C et al. (2006) New azasterols against Trypanosoma brucei: role of 24-sterol methyltransferase in inhibitor action. Antimicrob Agents Ch 50: 2595-2601.
12. Ishida K, Rodrigues JCF, Ribeiro MD, Vila TV, De Souza W, Urbina JA, Nakamura CV & Rozental S (2009) Growth inhibition and ultrastructural alterations induced by a delta(24(25))-sterol methyltransferase inhibitors in Candida spp. isolates, including non-albincans organisms. BMC Microbiol 9: 74-85.
13. Kurohara ML, Kwong FK, Lebherz TB & Klaustermeyer WB (1991) Metronidazole hypersensitivity and oral desensitization. J Allergy Clin Immun 88: 279-280.
14. Land KM, Clemens DL & Johnson PJ (2001) Loss of multiple hydrogenosomal proteins associated with organelle metabolism and high-level drug resistance in trichomonads. Exp Parasitol 97: 102-110.
15. Lossick JG, Müller M & Gorrell TE (1986) In vitro drug susceptibility and doses of metronidazole required for cure in cases of refractory vaginal trichomoniasis. J Infect Dis 153: 948-955.
16. Madeiro da Costa RF & Benchimol M (2004) The effect of drugs on cell structure of Tritrichomonas foetus. Parasitol Res 92: 159-170.
17. Maia C, Attias M, Urbina J, Gilbert I, Magaraci F & De Souza W (2007) Azasterols impair Giardia lamblia proliferation and induces encystation. Biochem Bioph Res Co 363: 310-316.
18. Mariante RM, Guimarães CA, Linden R & Benchimol M (2003) Hydrogen peroxide induces caspase activation and programmed cell death in the amitochondrial Tritrichomonas foetus. Histochem Cell Biol 120: 129-141.
19. Mariante RM, Vancini RG & Benchimol M (2006) Cell death in trichomonads: new insights. Histochem Cell Biol 125: 545-556.
20. Palmié-Peixoto IV, Rocha MR, Urbina JA, De Souza W, Einicker-Lamas M & Motta MC (2006) Effects of sterol biosynthesis inhibitors on endosymbiont- bearing trypanossomatids. FEMS Microbiol Lett 255: 33-42.
21. Pereira-Neves A, Ribeiro KC & Benchimol M (2003) Pseudocysts in trichomonads - new insights. Protist 154: 313-329.
22. Petrin D, Delgaty K, Bhatt R & Garber G (1998) Clinical and microbiological aspects of Trichomonas vaginalis. Clin Microbiol 11: 300-317.
23. Robinson SC (1962) Trichomonal vaginitis resistant to metranidazole. Can Med Assoc J 86: 665.
24. Rodrigues JC, Attias M, Rodriguez C, Urbina JA & Souza W (2002) Ultrastructural and biochemical alterations induced by 22, 26-azasterol, a delta(24(25))-sterol methyltransferase inhibitor, on promastigote and amastigote forms of Leishmania amazonensis. Antimicrob Agents Ch 46: 487-499.
25. Smilack JD, Wilson WR & Cockerill FR III (1991) Tetracyclines, chloramphenicol, erythromycin, clindamycin, and metronidazole. Mayo Clin Proc 66: 1270-1280.
26. Song Z & Nes WD (2007) Sterol biosynthesis inhibitors: potential for transition state analogs and mechanism-based inactivators targeted at sterol methyltransferase. Lipids 42: 15-33.
27. Sutcliffe S, Alderete JF, Till C, Goodman PJ, Hsing AW, Zenilman JM, De Marzo AM & Platz EA (2009) Trichomonosis and subsequent risk of prostate cancer in the prostate cancer prevention trial. Int J Cancer 124: 2082-2087.
28. Urbina JA (1997) Lipid biosynthesis pathways as chemotherapeutic targets in kinetoplastid parasites. Parasitology 114: S91-S99.
29. Urbina JA, Vivas J, Visbal G & Contreras LM (1995) Modification of the sterol composition of Trypanosoma (Schizotrypanum) cruzi epimastigotes by delta 24(25)-sterol methyl transferase inhibitors and their combinations with ketoconazole. Mol Biochem Parasit 73: 199-210.
30. Urbina JA, Visbal G, Contreras LM, McLaughlin G & Docampo R (1997) Inhibitors of delta24(25) sterol methyltransferase block sterol synthesis and cell proliferation in Pneumocystis carinii. Antimicrob Agents Ch 4: 428-432.
31. Visbal G, Alvarez A, Moreno B & San-Blas G (2003) S-Adenosyl-l-methionine inhibitors delta(24)-sterol methyltransferase and delta(24(28))-sterol methylreductase as possible agents against Paracoccidioides brasiliensis. Antimicrob Agents Ch 47: 2966-2970.
32. WHO (2001) Trichomoniasis. Global prevalence and incidence of selected curable sexually transmitted infections. Global program on AIDS, pp 2-27. World Health Organization, Geneva, Switzerland.
33. Wright JM, Webb RI, O'Donoghue P, Upcroft P & Upcroft JA (2010) Hydrogenosomes of laboratory-induced metronidazole-resistant Trichomonas vaginalis lines are downsized while those from clinically metronidazole-resistant isolates are not. J Eukaryot Microbiol 57: 171-176.