Cloning and expression in E. coli of an organic solvent-tolerant and alkali-resistant glucose 1-dehydrogenase from Lysinibacillus sphaericus G10

Bioresource Technology

Volumn 102, Issue 2, 2011, Pages 1528-1536

  Ding, Hai Tao ^a   Du, Yi Qing ^a   Liu, Dan Feng ^a   Li, Ze Li ^a   Chen, Xue Jiao ^a   Zhao, Yu Hua ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Alkaline resistance; Coenzyme regeneration; Glucose 1 dehydrogenase; Organic solvent tolerance

Indexed keywords

ALKALI RESISTANT; ALKALINE CONDITIONS; ALKALINE-RESISTANCE; CLONING AND EXPRESSION; COENZYME REGENERATION; E. COLI; ENZYME STABILITY; GAUSSIAN DISTRIBUTION MODEL; HIGH STABILITY; ISO-PROPANOLS; N-HEXANE; N-HEXANOL; N-PROPANOL; SODIUM PHOSPHATE BUFFER;

ACETONE; ALKALINITY; CLONING; ESCHERICHIA COLI; ETHANOL; GENE ENCODING; GLUCOSE; HEXANE; METHANOL; SODIUM; SODIUM CHLORIDE;

ORGANIC SOLVENTS;

2 PROPANOL; ACETONE; ALCOHOL; ALKALI; GLUCOSE DEHYDROGENASE; HEXANE; HEXANOL; METHANOL; ORGANIC SOLVENT; PROPANOL; SODIUM DIHYDROGEN PHOSPHATE;

ALCOHOL; ALKALINE ENVIRONMENT; BACTERIUM; CATION; ENZYME ACTIVITY; GAUSSIAN METHOD; GENE EXPRESSION; GLUCOSE; NUMERICAL MODEL; PH; RECOMBINATION; SODIUM CHLORIDE; TOLERANCE;

ARTICLE; CLONING; COENZYME; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; INCUBATION TEMPERATURE; LYSINIBACILLUS SPHAERICUS; NONHUMAN; NORMAL DISTRIBUTION; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ALKALIES; AMINO ACID SEQUENCE; BACILLUS; CLONING, MOLECULAR; EDETIC ACID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; ESCHERICHIA COLI; GENES, BACTERIAL; GLUCOSE 1-DEHYDROGENASE; HYDROGEN-ION CONCENTRATION; IONS; KINETICS; METALS; MOLECULAR SEQUENCE DATA; ORGANIC CHEMICALS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SOLVENTS; SUBSTRATE SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI;

EID: 78650705745     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.08.018     Document Type: Article

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