Rapid, highly efficient extraction and purification of membrane proteins using a microfluidic continuous-flow based aqueous two-phase system

Journal of Chromatography A

Volumn 1218, Issue 1, 2011, Pages 171-177

  Hu, Rui ^a   Feng, Xiaojun ^a   Chen, Pu ^a   Fu, Meng ^b   Chen, Hong ^c   Guo, Lin ^b   Liu, Bi Feng ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b WUHAN UNIVERSITY   (China)

^c INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Aqueous two phase system; Membrane proteins; Microfluidic chip; Purification

Indexed keywords

ANALYSIS TIME; AQUEOUS TWO PHASE SYSTEM; CELL EXTRACTS; CELLULAR FUNCTION; CONTINUOUS EXTRACTION; CONTINUOUS-FLOW; CONVENTIONAL APPROACH; DETECTION METHODS; EXTRACTION EFFICIENCIES; INTEGRAL MEMBRANE PROTEINS; MEMBRANE PROTEINS; MEMBRANE-ASSOCIATED PROTEINS; MICROFLUIDIC CHIP; NANOHPLC; PROTEOMICS; PURIFIED PROTEIN; SAMPLE CONSUMPTION; SDS-PAGE; SWISS-PROT; TRANS-MEMBRANE DOMAINS; TWO PHASE SYSTEMS;

ACTIVATION ANALYSIS; BIOLOGICAL MEMBRANES; CAPILLARY ELECTROPHORESIS; EMULSIFICATION; EXTRACTION; FLUIDIC DEVICES; LAMINAR FLOW; MICROFLUIDICS; MOLECULAR BIOLOGY; PURIFICATION; SEARCH ENGINES;

PROTEINS;

MEMBRANE PROTEIN;

ARTICLE; CAPILLARY ELECTROPHORESIS; CENTRIFUGATION; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; FLOW RATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; HUMIDITY; HYDROPHILICITY; HYDROPHOBICITY; MICROFLUIDICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN PURIFICATION; PROTEOMICS; TANDEM MASS SPECTROMETRY;

CELL EXTRACTS; CHEMICAL FRACTIONATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DATABASES, PROTEIN; ELECTROPHORESIS, CAPILLARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EQUIPMENT DESIGN; HELA CELLS; HUMANS; MEMBRANE PROTEINS; MICROFLUIDIC ANALYTICAL TECHNIQUES; PROTEOMICS; TANDEM MASS SPECTROMETRY;

EID: 78650418004     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.10.090     Document Type: Article

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