An insertional trap for conditional gene expression in Toxoplasma gondii: Identification of TAF250 as an essential gene

Molecular and Biochemical Parasitology

Volumn 175, Issue 2, 2011, Pages 133-143

  Jammallo, Lauren ^a   Eidell, Keith ^a   Davis, Paul H ^b,c   Dufort, Fay J ^a   Cronin, Courtney ^a   Thirugnanam, Sivasakthivel ^a   Chiles, Thomas C ^a   Roos, David S ^b   Gubbels, Marc Jan ^a  

^a BOSTON COLLEGE   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c UNIVERSITY OF NEBRASKA AT OMAHA   (United States)

Author keywords

Cell cycle; Chaperonin; Genetic screen; Invasion; TAF250; Toxoplasma gondii

Indexed keywords

CHAPERONIN; RNA POLYMERASE II; TAF250 ENZYME; TETRACYCLINE; UNCLASSIFIED DRUG;

ARTICLE; CELL CYCLE; CONTROLLED STUDY; DNA REPAIR; GENE EXPRESSION; GENE IDENTIFICATION; GENE INSERTION; GENE LOCUS; GENE MAPPING; GENE MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PLASMID; PRIORITY JOURNAL; TOXOPLASMA GONDII;

GENE EXPRESSION; GENE EXPRESSION PROFILING; GENES, ESSENTIAL; GENETIC VECTORS; GENETICS, MICROBIAL; MICROARRAY ANALYSIS; PLASMIDS; TETRACYCLINE; TOXOPLASMA; TRANSCRIPTION FACTOR TFIID;

TOXOPLASMA; TOXOPLASMA GONDII;

EID: 78650305327     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2010.10.007     Document Type: Article

References (43)

1. J.G. Montoya, and O. Liesenfeld Toxoplasmosis Lancet 363 2004 1965 1976
2. M.J. Gubbels, M. White, and T. Szatanek The cell cycle and Toxoplasma gondii cell division: tightly knit or loosely stitched? Int J Parasitol 38 2008 1343 1358
3. B. Striepen, C.N. Jordan, S. Reiff, and G.G. van Dooren Building the perfect parasite: cell division in apicomplexa PLoS Pathog 3 2007 e78
4. M.W. White, J. Radke, M. Conde de Felipe, and M. Lehmann Cell cycle control/parasite division 2007 Horizon Scientific Press Norwich, UK
5. M. Meissner, D. Schluter, and D. Soldati Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion Science 298 2002 837 840 (Pubitemid 35231538)
6. M. Meissner, S. Brecht, H. Bujard, and D. Soldati Modulation of myosin A expression by a newly established tetracycline repressor-based inducible system in Toxoplasma gondii Nucleic Acids Res 29 2001 E115
7. N.F. van Poppel, J. Welagen, R.F. Duisters, A.N. Vermeulen, and D. Schaap Tight control of transcription in Toxoplasma gondii using an alternative tet repressor Int J Parasitol 2006
8. A. Herm-Gotz, C. Agop-Nersesian, and S. Munter Rapid control of protein level in the apicomplexan Toxoplasma gondii Nat Methods 4 2007 1003 1005
9. M.J. Gubbels, M. Lehmann, and M. Muthalagi Forward genetic analysis of the apicomplexan cell division cycle in Toxoplasma gondii PLoS Pathog 4 2008 e36
10. L.J. Knoll, G.L. Furie, and J.C. Boothroyd Adaptation of signature-tagged mutagenesis for Toxoplasma gondii: a negative screening strategy to isolate genes that are essential in restrictive growth conditions Mol Biochem Parasitol 116 2001 11 16
11. D.S. Roos, R.G. Donald, N.S. Morrissette, and A.L. Moulton Molecular tools for genetic dissection of the protozoan parasite Toxoplasma gondii Methods Cell Biol 45 1994 27 63
12. M.J. Gubbels, C. Li, and B. Striepen High-throughput growth assay for Toxoplasma gondii using yellow fluorescent protein Antimicrob Agents Chemother 47 2003 309 316
13. M.J. Gubbels, S. Vaishnava, N. Boot, J.F. Dubremetz, and B. Striepen A MORN-repeat protein is a dynamic component of the Toxoplasma gondii cell division apparatus J Cell Sci 119 2006 2236 2245
14. B. Striepen, M.W. White, and C. Li Genetic complementation in apicomplexan parasites Proc Natl Acad Sci USA 99 2002 6304 6309
15. K.L. Carey, N.J. Westwood, T.J. Mitchison, and G.E. Ward A small-molecule approach to studying invasive mechanisms of Toxoplasma gondii Proc Natl Acad Sci USA 101 2004 7433 7438
16. A. Lorestani, L. Sheiner, K. Yang, S.D. Robertson, N. Sahoo, C.F. Brooks, D.J.P. Ferguson, B. Striepen, and M.-J. Gubbels A toxoplasma MORN1 null mutant is defective in basal complex assembly and apicoplast division PLoS ONE 5 2010 e12302
17. Anderson-White BR, Ivey FD, Cheng K, Szatanek T, Lorestani A, Beckers CJ, Ferguson DJP, Sahoo N, Gubbels MJ. A family of intermediate filament-like proteins is sequentially assembled into the cytoskeletal scaffold of Toxoplasma gondii. Cell Microbiol; in press, doi:10.1111/j.1462-5822.2010.01514.x.
18. M.S. Behnke, J.B. Radke, A.T. Smith, W.J. Sullivan Jr., and M.W. White The transcription of bradyzoite genes in Toxoplasma gondii is controlled by autonomous promoter elements Mol Microbiol 68 2008 1502 1518
19. M.A. Larkin, G. Blackshields, and N.P. Brown Clustal W and Clustal X version 2.0 Bioinformatics 23 2007 2947 2948
20. K. Tamura, J. Dudley, M. Nei, and S. Kumar MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 Mol Biol Evol 24 2007 1596 1599
21. R.G. Donald, and D.S. Roos Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria Proc Natl Acad Sci USA 90 1993 11703 11707
22. J.R. Radke, B. Striepen, M.N. Guerini, M.E. Jerome, D.S. Roos, and M.W. White Defining the cell cycle for the tachyzoite stage of Toxoplasma gondii Mol Biochem Parasitol 115 2001 165 175
23. M. Nishi, K. Hu, J.M. Murray, and D.S. Roos Organellar dynamics during the cell cycle of Toxoplasma gondii J Cell Sci 121 2008 1559 1568
24. C. Spiess, A.S. Meyer, S. Reissmann, and J. Frydman Mechanism of the eukaryotic chaperonin: protein folding in the chamber of secrets Trends Cell Biol 14 2004 598 604
25. K.I. Brackley, and J. Grantham Activities of the chaperonin containing TCP-1 (CCT): implications for cell cycle progression and cytoskeletal organisation Cell Stress Chaperones 14 2009 23 31
26. S.E. Dixon, K.L. Stilger, E.V. Elias, A. Naguleswaran, and W.J. Sullivan Jr. A decade of epigenetic research in Toxoplasma gondii Mol Biochem Parasitol 173 2010 1 9
27. S.R. Albright, and R. Tjian TAFs revisited: more data reveal new twists and confirm old ideas Gene 242 2000 1 13
28. Z. Bozdech, M. Llinas, B.L. Pulliam, E.D. Wong, J. Zhu, and J.L. DeRisi The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum PLoS Biol 1 2003 E5
29. K.G. Le Roch, Y. Zhou, and P.L. Blair Discovery of gene function by expression profiling of the malaria parasite life cycle Science 301 2003 1503 1508
30. M.S. Behnke, J.C. Wootton, M.M. Lehmann, J.B. Radke, O. Lucas, J. Nawas, L.D. Sibley, and M.W. White Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii PLoS One. 5 2010 e12354
31. M.A. McAleer, A.J. Coffey, and I. Dunham DNA rescue by the vectorette method Methods Mol Biol 226 2003 393 400
32. J.L. Li, and L.S. Cox Identification of an MCM4 homologue expressed specifically in the sexual stage of Plasmodium falciparum Int J Parasitol 31 2001 1246 1252
33. J.P. Murry, C.M. Sassetti, J.M. Lane, Z. Xie, and E.J. Rubin Transposon site hybridization in Mycobacterium tuberculosis Methods Mol Biol 416 2008 45 59
34. R.G. Donald, and D.S. Roos Insertional mutagenesis and marker rescue in a protozoan parasite: cloning of the uracil phosphoribosyltransferase locus from Toxoplasma gondii Proc Natl Acad Sci USA 92 1995 5749 5753
35. M.B. Frankel, D.G. Mordue, and L.J. Knoll Discovery of parasite virulence genes reveals a unique regulator of chromosome condensation 1 ortholog critical for efficient nuclear trafficking Proc Natl Acad Sci USA 104 2007 10181 10186
36. G. Arrizabalaga, F. Ruiz, S. Moreno, and J.C. Boothroyd Ionophore-resistant mutant of Toxoplasma gondii reveals involvement of a sodium/hydrogen exchanger in calcium regulation J Cell Biol 165 2004 653 662
37. P.J. Hastings, J.R. Lupski, S.M. Rosenberg, and G. Ira Mechanisms of change in gene copy number Nat Rev Genet 10 2009 551 564
38. A.R. Quinlan, R.A. Clark, and S. Sokolova Genome-wide mapping and assembly of structural variant breakpoints in the mouse genome Genome Res 20 2010 623 635
39. G. Hynes, C.W. Sutton, and U.S.K.R. Willison Peptide mass fingerprinting of chaperonin-containing TCP-1 (CCT) and copurifying proteins FASEB J 10 1996 137 147
40. D. Ursic, J.C. Sedbrook, K.L. Himmel, and M.R. Culbertson The essential yeast Tcp1 protein affects actin and microtubules Mol Biol Cell 5 1994 1065 1080
41. J.M. Dobrowolski, and L.D. Sibley Toxoplasma invasion of mammalian cells is powered by the actin cytoskeleton of the parasite Cell 84 1996 933 939
42. M.K. Shaw, H.L. Compton, D.S. Roos, and L.G. Tilney Microtubules, but not actin filaments, drive daughter cell budding and cell division in Toxoplasma gondii J Cell Sci 113 Pt 7 2000 1241 1254
43. G.E. Chalkley, and C.P. Verrijzer DNA binding site selection by RNA polymerase II TAFs: a TAF(II)250-TAF(II)150 complex recognizes the initiator EMBO J 18 1999 4835 4845