Transposon site hybridization in Mycobacterium tuberculosis

Methods in Molecular Biology

Volumn 416, Issue , 2007, Pages 45-59

  Murry, Jeffrey P ^a   Sassetti, Christopher M ^a   Lane, James M ^a   Xie, Zhifang ^a   Rubin, Eric J ^a  

^a NONE

Author keywords

Method design; Microarray; Mycobacterium tuberculosis; Transposon; TraSH

Indexed keywords

ARTICLE; BACTERIAL GENOME; DNA MICROARRAY; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METHODOLOGY; MYCOBACTERIUM TUBERCULOSIS; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; TRANSPOSON;

DNA TRANSPOSABLE ELEMENTS; GENOME, BACTERIAL; MUTAGENESIS, INSERTIONAL; MYCOBACTERIUM TUBERCULOSIS; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; POLYMERASE CHAIN REACTION;

CORYNEBACTERINEAE; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS;

EID: 40549111907     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (14)

1. Badarinarayana, V., Estep, P. W. 3rd, Shendure, J., Edwards, J., Tavazoie, S., Lam, F., and Church, G. M. (2001) Selection analyses of insertional mutants using subgenic-resolution arrays. Nat. Biotechnol. 19, 1060-1065.
2. Sassetti, C. M., Boyd, D. H., and Rubin, E. J. (2001) Comprehensive identification of conditionally essential genes in mycobacteria. Proc. Natl. Acad. Sci. U.S.A. 98, 12,712-12,717.
3. Salama, N. R., Shepherd, B., and Falkow, S. (2004) Global, transposon mutagenesis and essential, gene analysis of Helicobacter pylori. J. Bacteriol. 186, 7926-7935.
4. Chan, K., Kim, C. C., and Falkow, S. (2005) Microarray-based detection of Salmonella enterica serovar Typhimurium transposon mutants that cannot survive in macrophages and mice. Infect. Immun. 73, 5438-5449.
5. Rengarajan, J., Bloom, B. R., and Rubin, E. J. (2005) Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages. Proc. Natl. Acad. Sci. U.S.A. 102, 8327-8332.
6. Sassetti, C. M., and Rubin, E. J. (2003) Genetic requirements for mycobacterial survival during infection. Proc. Natl. Acad. Sci. U.S.A. 100, 12,989-12,994.
7. Sassetti, C. M., Boyd, D. IL, and Rubin, E. J. (2003) Genes required for mycobacterial growth defined by high density mutagenesis. Mol. Microbiol. 48, 77-84.
8. Lawley, T. D., Chan, K., Thompson, L. J., Kim, C. C., Govoni, G. R., and Monack, D. M. (2006) Genome-wide screen for salmonella genes required for long-term systemic infection of the mouse. PLoS Pathog. 2, e11.
9. Bardarov, S., Kriakov, J., Carriere, C., Yu, S., Vaamonde, C., McAdam, R. A., et al. (1997) Conditionally replicating mycobacteriophages: a system for transposon delivery to Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. U.S.A. 94, 10,961-10,966.
10. Lampe, D. J., Akerley, B. J., Rubin, E. J., Mekalanos, J. J., and Robertson, H. M. (1999) Hyperactive transposase mutants of the Himar1 mariner transposon. Proc. Natl. Acad. Sci. U.S.A. 96, 11,428-11,433.
11. Rubin, E. J., Akerley, B. J., Novik, V. N., Lampe, D. J., Husson, R. N., and Mekalanos, J. J. (1999) In vivo transposition of manner-based elements in enteric bacteria and mycobacteria. Proc. Natl. Acad. Sci. U.S.A. 96, 1645-1650.
12. Belisle, J. T., and Sonnenberg, M. G. (1998) Isolation of genomic DNA from mycobacteria. Methods Mol. Biol. 101, 31-44.
13. Rozen, S., and Skaletsky, H. (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 132, 365-386.
14. Yang, Y. H., Dudoit, S., Luu, P., and Speed, T. P. (2001) Normalization for cDNA microarray data. Presented at SPIE BiOS 2001, San Jose, California.