Processive replication of single DNA molecules in a nanopore catalyzed by phi29 DNA polymerase

Journal of the American Chemical Society

Volumn 132, Issue 50, 2010, Pages 17961-17972

  Lieberman, Kate R ^a   Cherf, Gerald M ^a   Doody, Michael J ^a   Olasagasti, Felix ^a   Kolodji, Yvette ^a   Akeson, Mark ^a  

^a University of California Santa Cruz   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOPHAGE PHI29; DNA COMPLEX; DNA SUBSTRATES; DNA SYNTHESISS; DNA-TEMPLATE; ENZYME COMPLEXES; EXONUCLEASE ACTIVITY; HIGH AFFINITY; HIGH BANDWIDTH; NANO-SCALE PORES; PHI29 DNA POLYMERASE; PRIMER STRAND; PROCESSIVITY; REAL TIME; REAL-TIME DETECTION; SINGLE DNA MOLECULES; SPATIAL RESOLUTION; TEMPLATE STRAND; TERMINAL RESIDUES; WILD TYPES;

ELECTRIC FIELDS; ENZYMES; NANOPORES; NUCLEIC ACIDS; RNA;

DNA;

ALPHA HEMOLYSIN; DEOXYRIBONUCLEOTIDE; DNA POLYMERASE; EXODEOXYRIBONUCLEASE III; EXODEOXYRIBONUCLEASE V;

ARTICLE; BACTERIOPHAGE; CATALYSIS; DNA REPLICATION; DNA SYNTHESIS; DNA TEMPLATE; ELECTRIC FIELD; ENZYME ACTIVITY; NANOPORE;

CATALYSIS; DNA REPLICATION; DNA-DIRECTED DNA POLYMERASE; MODELS, BIOLOGICAL; NANOPORES; SUBSTRATE SPECIFICITY; VIRAL PROTEINS;

EID: 78650280872     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja1087612     Document Type: Article

References (31)

1. Kapanidis, A. N. and Strick, T. Trends. Biochem. Sci. 2009, 34, 234-243
2. Moffitt, J. R., Chemla, Y. R., Smith, S. B., and Bustamante, C. Annu. Rev. Biochem. 2008, 77, 205-228
3. Myong, S. and Ha, T. Curr. Opin. Struct. Biol. 2010, 20, 121-127
4. Eid, J.; et al. Science 2009, 323, 133 - 138
5. Harris, T. D.; et al. Science 2008, 320, 106 - 109.
6. Kasianowicz, J. J., Brandin, E., Branton, D., and Deamer, D. W. Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 13770-13773
7. Akeson, M., Branton, D., Kasianowicz, J. J., Brandin, E., and Deamer, D. W. Biophys. J. 1999, 77, 3227-3233
8. Ma, L. and Cockroft, S. L. ChemBioChem 2010, 11, 25-34
9. Meller, A., Nivon, L., Brandin, E., Golovchenko, J., and Branton, D. Proc. Natl. Acad. Sci. U.S.A. 2000, 97, 1079-1084
10. Hornblower, B., Coombs, A., Whitaker, R. D., Kolomeisky, A., Picone, S. J., Meller, A., and Akeson, M. Nat. Methods 2007, 4, 315-317
11. Astier, Y., Kainov, D. E., Bayley, H., Tuma, R., and Howorka, S. ChemPhysChem 2007, 8, 2189-2194
12. Benner, S., Chen, R. J., Wilson, N. A., Abu-Shumays, R., Hurt, N., Lieberman, K. R., Deamer, D. W., Dunbar, W. B., and Akeson, M. Nat. Nanotechnol. 2007, 2, 718-724
13. Cockroft, S. L., Chu, J., Amorin, M., and Ghadiri, M. R. J. Am. Chem. Soc. 2008, 130, 818-820
14. Gyarfas, B., Olasagasti, F., Benner, S., Garalde, D., Lieberman, K. R., and Akeson, M. ACS Nano 2009, 3, 1457-1466
15. Hurt, N., Wang, H., Akeson, M., and Lieberman, K. R. J. Am. Chem. Soc. 2009, 131, 3772-3778
16. Wilson, N. A., Abu-Shumays, R., Gyarfas, B., Wang, H., Lieberman, K. R., Akeson, M., and Dunbar, W. B. ACS Nano 2009, 3, 995-1003
17. Olasagasti, F., Lieberman, K. R., Benner, S., Cherf, G. M., Dahl, J. M., Deamer, D. W., and Akeson, M. Nat. Nanotechnol. 2010, 5, 798 - 806.
18. Blanco, L. and Salas, M. J. Biol. Chem. 1996, 271, 8509-8512
19. Salas, M., Blanco, L., Lázaro, J. M., and de Vega, M. IUBMB Life 2008, 60, 82-85
20. Salas, M. Annu. Rev. Biochem. 1991, 60, 39-71
21. Blanco, L., Bernad, A., Lázaro, J. M., Martín, G., Garmendia, C., and Salas, M. J. Biol. Chem. 1989, 264, 8935-8940
22. Berman, A. J., Kamtekar, S., Goodman, J. L., Lázaro, J. M., de Vega, M., Blanco, L., Salas, M., and Steitz, T. A. EMBO J. 2007, 26, 3494-3505
23. Kamtekar, S., Berman, A. J., Wang, J., Lázaro, J. M., de Vega, M., Blanco, L., Salas, M., and Steitz, T. A. Mol. Cell 2004, 16, 609-618
24. Johnson, A. and O'Donnell, M. Annu. Rev. Biochem. 2005, 74, 283-315
25. Rodríguez, I., Lázaro, J. M., Blanco, L., Kamtekar, S., Berman, A. J., Wang, J., Steitz, T. A., Salas, M., and de Vega, M. Proc. Natl. Acad. Sci. U.S.A. 2005, 102, 6407-6412
26. Ibarra, B., Chemla, Y. R., Plyasunov, S., Smith, S. B., Lázaro, J. M., Salas, M., and Bustamante, C. EMBO J. 2009, 28, 2794-2802
27. Vercoutere, W., Winters-Hilt, S., Olsen, H., Deamer, D., Haussler, D., and Akeson, M. Nat. Biotechnol. 2001, 19, 248-252
28. Blasco, M. A., Lázaro, J. M., Blanco, L., and Salas, M. J. Biol. Chem. 1993, 268, 16763-16770
29. Blanco, L. and Salas, M. Nucleic Acids Res. 1985, 13, 1239-249
30. Garmendia, C., Bernad, A., Esteban, J. A., Blanco, L., and Salas, M. J. Biol. Chem. 1992, 267, 2594-2599
31. Truniger, V., Lázaro, J. M., Esteban, F. J., Blanco, L., and Salas, M. Nucleic Acids Res. 2002, 30, 1483-1492