A study on the temperature dependency and time course of the cold capture antibody secretion assay

Journal of Biotechnology

Volumn 141, Issue 1-2, 2009, Pages 80-83

  Pichler, Johannes ^a   Hesse, Friedemann ^a   Wieser, Matthias ^a   Kunert, Renate ^a   Galosy, Sybille S ^b   Mott, John E ^b   Borth, Nicole ^a  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

^b PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United States)

Author keywords

Flow cytometric cell sorting; High production rates; Recombinant CHO cells; Single cell secretion assay

Indexed keywords

CELL SURFACES; FLOW CYTOMETRIC CELL SORTING; FLUORESCENT ANTIBODIES; FLUORESCENT LABELS; FLUORESCENT SIGNALS; HEAVY CHAINS; HIGH PRODUCTION RATES; HIGH PRODUCTIVITIES; HUMAN ANTIBODIES; LOW TEMPERATURES; PH DEPENDENTS; PRODUCT RELEASE; PROTEIN RELEASE; RECOMBINANT CELLS; RECOMBINANT CHO CELLS; ROOM TEMPERATURES; SECRETED PROTEINS; SIGNAL INTENSITIES; SIGNAL STABILITIES; SIMPLE METHODS; SINGLE CELL SECRETION ASSAY; SMALL MOLECULES; SPECIFIC PRODUCTION RATES; SPECIFIC PRODUCTIVITIES; TEMPERATURE DEPENDENCIES; TIME COURSE; VESICLE FUSIONS;

ANTIBODIES; ASSAYS; CELL CULTURE; CELL MEMBRANES; CONCENTRATION (PROCESS);

FLUORESCENCE;

ANTIBODY; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENT DYE; PHYCOERYTHRIN;

ANTIBODY PRODUCTION; ARTICLE; CELL COMPARTMENTALIZATION; CELL SURFACE; CHO CELL; CONTROLLED STUDY; CYTOPLASM; ENDOCYTOSIS; FLUORESCENCE; HEAVY CHAIN; HUMAN; LIGHT CHAIN; LOW TEMPERATURE; PH; PRIORITY JOURNAL; PROTEIN SECRETION; ROOM TEMPERATURE; STAINING; TEMPERATURE DEPENDENCE;

ANIMALS; ANTIBODIES; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; FLOW CYTOMETRY; MICROSCOPY, FLUORESCENCE; TEMPERATURE; TRANSFECTION;

EID: 64249163225     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.03.001     Document Type: Article

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