Screening for improved cell performance: Selection of subclones with altered production kinetics or improved stability by cell sorting

Biotechnology and Bioengineering

Volumn 88, Issue 6, 2004, Pages 699-706

  Böhm, Ernst ^a   Voglauer, Regina ^a   Steinfellner, Willi ^a   Kunert, Renate ^a   Borth, Nicole ^a   Katinger, Hermann ^a  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

Author keywords

Cell sorting; Flow cytometry; Human monoclonal antibody; Production kinetics; Recombinant Chinese hamster ovary cells; Stability

Indexed keywords

BIOREACTORS; CLONING; GROWTH KINETICS; PROTEINS;

BIOPHARMACEUTICAL PROTEINS; CELL LINE; CELL PERFORMANCE; SUBCLONES;

CELL CULTURE;

MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BIOTECHNOLOGICAL PROCEDURES; CELL ASSAY; CELL CULTURE; CELL GROWTH; CELL KINETICS; CELL SELECTION; CELL SPECIFICITY; CELL SURFACE; CHO CELL; CONTROLLED STUDY; FLOW CYTOMETRY; MOLECULAR CLONING; MOLECULAR STABILITY; NONHUMAN; PRESSURE MEASUREMENT; SINGLE CELL SECRETION ASSAY; ANIMAL; BIOSYNTHESIS; CELL SEPARATION; CRICETULUS; ENZYME LINKED IMMUNOSORBENT ASSAY; EVALUATION; GENETIC ENHANCEMENT; GENETICS; HAMSTER; METHODOLOGY; MICROFLUIDIC ANALYSIS; PROTEIN ENGINEERING;

CRICETINAE; CRICETULUS GRISEUS;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL SEPARATION; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GENETIC ENHANCEMENT; MICROFLUIDIC ANALYTICAL TECHNIQUES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 16644380307     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20271     Document Type: Article

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