Identification of novel halotolerant bacillopeptidase F-like proteinases from a moderately halophilic bacterium, Virgibacillus sp. SK37

Journal of Applied Microbiology

Volumn 110, Issue 1, 2011, Pages 191-201

  Phrommao, E ^a   Rodtong, S ^a   Yongsawatdigul, J ^a  

^a SURANAREE UNIVERSITY OF TECHNOLOGY   (Thailand)

Author keywords

Bacillopeptidase F; Fish sauce; Halotolerant enzymes; Moderately halophilic bacteria; Na activated proteinases; Subtilisin like proteinases; Virgibacillus sp.

Indexed keywords

BACTERIA; ELECTROPHORESIS; ETHYLENEDIAMINETETRAACETIC ACID; FISH; FLUORINE COMPOUNDS; PEPTIDES; SODIUM CHLORIDE;

BACILLOPEPTIDASE F; FISH SAUCE; HALOPHILIC BACTERIA; HALOTOLERANT; HALOTOLERANT ENZYME; MODERATELY HALOPHILIC BACTERIA; NA-ACTIVATED PROTEINASE; SUBTILISIN; SUBTILISIN-LIKE PROTEINASE; VIRGIBACILLUS SP; VIRGIBACILLUS SP.;

ENZYMES;

BACILLOPEPTIDASE F; EDETIC ACID; PROTEINASE; SUBTILISIN; UNCLASSIFIED DRUG;

BACTERIUM; BIOTECHNOLOGY; EDTA; ELECTROKINESIS; ENZYME ACTIVITY; FISH; IDENTIFICATION METHOD; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDE; PH; SALINITY TOLERANCE; SODIUM CHLORIDE;

ARTICLE; ENZYME ACTIVITY; ENZYME STABILITY; HALOPHILIC BACTERIUM; ISOELECTRIC POINT; NONHUMAN; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SALT TOLERANCE; SEQUENCE HOMOLOGY; TWO DIMENSIONAL ELECTROPHORESIS; VIRGIBACILLUS;

BACTERIA (MICROORGANISMS); VIRGIBACILLUS;

EID: 78650156262     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2010.04871.x     Document Type: Article

References (46)

1. Bode, W., Papamokos, E. and Musil, D. (1987) The high-resolution X-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hirudo medicinalis structural analysis, subtilisin structure and interface geometry. Eur J Biochem 166, 673-692.
2. Bott, R., Ultsch, M., Kossiakoff, A., Graycar, T., Katz, B. and Power, S. (1988) The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A° and an analysis of the structural consequences of peroxide inactivation. J Biol Chem 263, 7895-7906.
3. Boyer, H.W. and Carlton, B.C. (1968) Production of two proteolytic enzymes by a transformable strain of Bacillus subtilis. Arch Biochem Biophys 128, 422-455.
4. Carrasco, I.J., Márquez, M.C. and Ventosa, A. (2009) Virgibacillus salinus sp. nov., a moderately halophilic bacterium from sediment of a saline lake. Int J Syst Evol Microbiol 59, 3068-3073.
5. Chamroensaksri, N., Akaracharanya, A., Visessanguan, W. and Tanasupawat, S. (2008) Characterization of halophilic bacterium NB2-1 from Pla-Ra and its protease production. J Food Biochem 32, 536-555.
6. Chen, Y.G., Cui, X.L., Wang, Y.X., Zhang, Y.Q., Tang, S.K., Li, W.J., Liu, Z.X., Wen, M.L. et al. (2009) Virgibacillus sediminis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 59, 2058-2063.
7. Graycer, T.P., Ballinger, M.D. and Wells, J.A. (2004) Subtilisins. In Handbook of Proteolytic Enzymes ed. Barrett, A.J., Rawlings, N.D. and Woessner, J.F. pp. 1786-1792. London: Elsevier.
8. Gupta, R., Beg, Q.K. and Lorenz, P. (2002) Bacterial alkaline proteases: molecular approaches and industrial applications. Appl Microbiol Biotechnol 59, 15-32.
9. Gupta, A., Joseph, B., Mani, A. and Thomas, G. (2008) Biosynthesis and properties of an extracellular thermostable serine alkaline proteinase from Virgibacillus pantothenticus. World J Microbiol Biotechnol 24, 237-243.
10. Hageman, J.H. (2004) Bacillopeptidase. In Handbook of Proteolytic Enzymes ed. Barrett, A.J., Rawlings, N.D. and Woessner, J.F. pp. 1795-1796. London: Elsevier.
11. Heukeshoven, J. and Dernick, R. (1985) Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6, 103-112.
12. Hiraga, K., Nishikata, Y., Namwong, S., Tanasupawat, S., Takada, K. and Oda, K. (2005) Purification and characterization of serine proteinase from a halophilic bacterium, Filobacillus sp. RF2-5. Biosci Biotechnol Biochem 69, 38-44.
13. Hua, N.P., Hamza, C.A., Vreeland, R.H., Isoda, H. and Naganuma, T. (2008) Virgibacillus salarius sp. nov., a halophilic bacterium isolated from a Saharan salt lake. Int J Syst Evol Microbiol 58, 2409-2414.
14. Hwang, K.J., Choi, K.H., Kim, M.J., Park, C.S. and Cha, J. (2007) Purification and characterization of a new fibrinolytic enzyme of Bacillus licheniformis KJ-31, isolated from Korean traditional Jeot-gal. J Microbiol Biotechnol 17, 1469-1476.
15. Joo, H.-S., Kumar, C.G., Park, G.-C., Paik, S.R. and Chang, C.-S. (2003) Oxidant and SDS-stable alkaline protease from Bacillus clausii I-52: production and some properties. J Appl Microbiol 95, 267-272.
16. Kim, W.J. and Kim, S.M. (2005) Purification and characterization of Bacillus subtilis JM-3 protease from anchovy sauce. J Food Biochem 29, 591-610.
17. Kobayashi, T., Hakamada, Y., Adachi, S., Hitomi, J., Yoshimatsu, T., Koike, K., Kawai, S. and Ito, S. (1995) Purification and properties of an alkaline protease from alkalophilic Bacillus sp. KSM-K16. Appl Microbiol Biotechnol 43, 473-481.
18. Kushner, D.J. (1978) Life in high salt and solute concentrations: halophilic bacteria. In Microbial Life in Extreme Environments ed. Kushner, D.J. pp. 317-368. London: Academic Press, Ltd.
19. Lama, L., Romano, I., Calandrelli, V., Nicolaus, B. and Gambacorta, A. (2005) Purification and characterization of a protease produced by an aerobic haloalkaliphilic species belonging to the Salinivibrio genus. Res Microbiol 156, 478-484.
20. Lanyi, J.K. (1974) Salt-dependent properties of proteins from extremely halophilic bacteria. Bacteriol Rev 38, 272-290.
21. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277, 680-685.
22. Namwong, S., Hiraga, K., Takada, K., Tsunemi, M., Tanasupawat, S. and Oda, K. (2006) A halophilic serine proteinase from Halobacillus sp. SR5-3 isolated from fish sauce: purification and characterization. Biosci Biotechnol Biochem 70, 1395-1401.
23. Neuhoff, V., Stamm, R. and Eibl, H. (1985) Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: a systematic analysis. Electrophoresis 6, 427-448.
24. Nonaka, T., Fujihashi, M., Kita, A., Saeki, K., Ito, S. and Miki, K. (2001) Crystallization and preliminary X-ray diffraction studies of a novel alkaline serine protease (KP-43) from alkaliphilic Bacillus sp. strain KSM-KP43. Acta Crystallogr 57, 717-718.
25. Okuda, M., Sumitomo, N., Takimura, Y., Ogawa, A., Saeki, K., Kawai, S., Kobayashi, T. and Ito, S. (2004) A new subtilisin family: nucleotide and deduced amino acid sequences of new high-molecular-mass alkaline proteases from Bacillus spp. Extremophiles 8, 229-235.
26. Omura, K., Hitosugi, M., Zhu, X., Ikeda, M., Maeda, H. and Tokudome, S. (2005) A newly derived protein from Bacillus subtilis natto with both antithrombotic and fibrinolytic effects. J Pharm Sci 99, 247-251.
27. Peng, Q.Z., Chen, J., Zhang, Y.Q., Chen, Q.H., Peng, D.J., Cui, X.L., Li, W.J. and Chen, Y.G. (2009) Virgibacillus zhanjiangensis sp. nov., a marine bacterium isolated from sea water. Antonie Van Leeuwenhoek 96, 645-652.
28. Rao, M.B., Tanksale, A.M., Ghatge, M.S. and Deshpande, V.V. (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62, 597-635.
29. Rawlings, N.D., Morton, F.R., Kok, C.Y., Kong, J. and Barrett, A.J. (2008) MEROPS: the peptidase database. Nucleic Acids Res 36, 320-325.
30. Rohban, R., Amoozegar, M.A. and Ventosa, A. (2009) Screening and isolation of halophilic bacteria producing extracellular hydrolyses from Howz Soltan Lake, Iran. J Ind Microbiol Biotechnol 36, 333-340.
31. Saeki, K., Mitsuyoshi, O., Hatada, Y., Kobayashi, T., Ito, S., Takami, H. and Horikoshi, K. (2000) Novel oxidatively stable subtilisin-like serine proteases from alkaliphilic Bacillus spp.: enzymatic properties, sequences, and evolutionary relationships. Biochem Biophys Res Commun 279, 313-319.
32. Setyorini, E., Takenaka, S., Murakami, S. and Aoki, K. (2006) Purification and characterization of two novel halotolerant extracellular proteases from Bacillus subtilis strain FP-133. Biosci Biotechnol Biochem 70, 433-440.
33. Siezen, R.J. and Leunissen, J.A.M. (1997) Subtilases: the superfamily of subtilisin-like serine proteases. Protein Sci 6, 501-523.
34. Siezen, R.J., De Vos, W.M., Leunissen, J.A.M. and Dijkstra, B.W. (1991) Homology modeling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteases. Protein Eng 4, 719-739.
35. Sinsuwan, S., Rodtong, S. and Yongsawatdigul, J. (2007) NaCl-activated extracellular proteinase from Virgibacillus sp. SK37 isolated from fish sauce fermentation. J Food Sci 72, 264-269.
36. Sinsuwan, S., Rodtong, S. and Yongsawatdigul, J. (2008) Production and characterization of NaCl-activated proteinases from Virgibacillus sp. SK33 isolated from fish sauce fermentation. Process Biochem 43, 185-192.
37. Sinsuwan, S., Rodtong, S. and Yongsawatdigul, J. (2010) A NaCl-stable serine proteinase from Virgibacillus sp. SK33 isolated from Thai fish sauce. Food Chem 119, 573-579.
38. Sloma, A., Rufo, G.A. Jr, Rudolph, C.F., Sullivan, B.J., Theriault, K.A. and Pero, J. (1990) Bacillopeptidase F of Bacillus subtilis: purification of the protein and cloning of the gene. J Bacteriol 172, 1470-1477.
39. Stauffer, C.E. and Etson, D. (1969) The effect on subtilisin activity of oxidizing a methionine residue. J Biol Chem 244, 5333-5338.
40. Suzuki, M., Taguchi, S., Yamada, S., Kojima, S., Miura, K.I. and Momose, H. (1997) A novel member of the subtilisin-like protease family from Streptomyces albogrisolus. J Bacteriol 179, 430-438.
41. Uchida, H., Kondo, D., Yamashita, S., Tanaka, T., Tran, L.H., Nagano, H. and Uwajima, T. (2004) Purification and properties of a protease produced by Bacillus subtilis CN2 isolated from a Vietnamese fish sauce. J Microbiol Biotechnol 20, 579-582.
42. Ventosa, A.J., Nieto, J. and Oren, A. (1998) Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62, 504-554.
43. Wang, C.Y., Chang, C.C., Ng, C.C., Chen, T.W. and Shyu, Y.T. (2008) Virgibacillus chiguensis sp. nov., a novel halophilic bacterium isolated from Chigu, a previously commercial saltern located in southern Taiwan. Int J Syst Evol Microbiol 58, 341-345.
44. Wu, J., Bian, Y., Tang, B., Chen, X., Shen, P. and Peng, Z. (2004) Cloning and analysis of WF146 protease, a novel thermophilic subtilisin-like protease with four inserted surface loops. FEMS Microbiol Lett 230, 251-258.
45. Yongsawatdigul, J., Rodtong, S. and Raksakulthai, N. (2007) Acceleration of Thai fish sauce fermentation using proteinases and bacterial starter cultures. J Food Sci 72, 382-390.
46. Yoon, J.H., Kang, S.J., Jung, Y.T., Lee, K.C., Oh, H.W. and Oh, T.K. (2010) Virgibacillus byunsanensis sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 60, 291-295.