Novel oxidatively stable subtilisin-like serine proteases from alkaliphilic Bacillus spp.: Enzymatic properties, sequences, and evolutionary relationships

Biochemical and Biophysical Research Communications

Volumn 279, Issue 2, 2000, Pages 313-319

  Saeki, Katsuhisa ^a   Okuda, Mitsuyoshi ^a   Hatada, Yuji ^a   Kobayashi, Tohru ^a   Ito, Susumu ^a   Takami, Hideto ^b   Horikoshi, Koki ^b  

^a KAO CORPORATION   (Japan)

^b JAPAN AGENCY FOR MARINE EARTH SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Alkaliphile; Evolution; Oxidatively stable protease; Phylogenetic tree; Subtilisin

Indexed keywords

ALKALINE PROTEINASE; SERINE PROTEINASE; SUBTILISIN;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ANALYSIS; ENZYME INACTIVATION; GENETIC ANALYSIS; MOLECULAR CLONING; MOLECULAR EVOLUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; PHYLOGENY; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STRAIN DIFFERENCE; THERMOSTABILITY;

BACILLUS;

EID: 0034694799     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.3931     Document Type: Article

References (26)

1. Molecular and biotechnological aspects of microbial proteases
2. Subtilases: The superfamily of subtilisin-like serine proteases
3. Production of alkaline enzymes by alkalophilic microorganisms. Part I. Alkaline protease produced by Bacillus no. 221
4. Crystal structure of the alkaline proteinase Savinase™ from Bacillus lentus at 1.4 Å resolution
5. Proteolytic compounds of alkaline proteases of Bacillus strains. Zymograms and electrophoretic isolation
6. Purification and properties of an alkaline protease from alkalophilic Bacillus sp. KSM-K16
7. Crystal structure of the high-alkaline serine protease PB92 from Bacillus alcalophilus
8. Structure of a new alkaline serine protease (M-protease) from Bacillus sp. KSM-K16
9. High-resolution crystal structure of M-protease: Phylogeny aided analysis of the high-alkaline adaptation mechanism
10. Subtilisin - An enzyme designed to be engineered
11. Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteases
12. Stabilization and rational design of serine protease AprM under highly alkaline and high-temperature conditions
13. The effect on subtilisin activity of oxidizing a methionine residue
14. The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 Å and an analysis of the structural consequences of peroxide inactivation
15. Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation
16. Molecular cloning and nucleotide sequence of the gene for an alkaline protease from the alkalophilic Bacillus sp. KSM-K16
17. Preparation of transforming deoxyribonucleic acid by phenol treatment
18. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
19. Clustal W: Improving the sensitivity of progressive multiple sequence alignments through sequence weighting, positions-specific gap penalties and weight matrix choice
20. A neighbor-joining method: A new method for reconstructing phylogenetic trees
21. Protein measurement with the Folin phenol reagent
22. Protein secretion in Bacillus species
23. Homology analysis of the propeptides of subtilisin-like serine proteases (subtilases)
24. Pro-protein convertases of subtilisin/kexin family
25. The prosegment-subtilisin BPN' complex: Crystal structure of a specific 'foldase'