Purification and characterization of a protease produced by an aerobic haloalkaliphilic species belonging to the Salinivibrio genus

Research in Microbiology

Volumn 156, Issue 4, 2005, Pages 478-484

  Lama, Licia ^a   Romano, Ida ^a   Calandrelli, Valeria ^a   Nicolaus, Barbara ^a   Gambacorta, Agata ^a  

^a CNR   (Italy)

Author keywords

Extremophiles; Haloalkaliphilic bacteria; Protease; Salinivibrio

Indexed keywords

BACTERIAL ENZYME; BENZYLSULFONYL FLUORIDE; CALCIUM CHLORIDE; DODECYL SULFATE SODIUM; PROTEINASE; SODIUM CHLORIDE; THIOL DERIVATIVE; UREA;

ALKALINITY; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM ISOLATE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; GROWTH CURVE; HALF LIFE TIME; HALOPHILIC BACTERIUM; MOLECULAR WEIGHT; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL; PROTEINASE INHIBITION; SALINIVIBRIO COSTICOLA COSTICOLA; TEMPERATURE DEPENDENCE;

SALINIVIBRIO; SALINIVIBRIO COSTICOLA;

EID: 18144369986     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.resmic.2004.12.004     Document Type: Article

References (25)

1. M.W.W. Adams, and R.M. Kelly Enzymes from microorganisms in extreme environments Chem. Eng. News 73 1995 32 42
2. M.M. Bradford A rapid and sensitive method for quantitation of microgram quantities of proteine utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
3. G. Catara, G. Ruggiero, F. La Cara, F.A. Digilio, A. Capasso, and M. Rossi A novel extracellular subtilisin-like protease from the hyperthermophile Aeropyrum pernix K1: Biochemical properties, cloning, and expression Extremophiles 7 2003 391 399
4. M.J. Coronado, C. Vargas, J. Hofemeister, A. Ventosa, and J.J. Nieto Production and biochemical characterization of an amylase from the moderate halophile Halomonas meridiana FEMS Microbiol. Lett. 183 2000 67 71
5. M.I. Gimenez, C.A. Studdert, J. J Sanchez, and R.E. De Castro Extracellular protease of Natrialba magadii: Purification and biochemical characterization Extremophiles 4 2000 181 188
6. G. Gnosspellius Purification and properties of an extracellular protease from Myxococcus virescens J. Bacteriol. 133 1978 17 25
7. R. Gupta, Q.R. Beg, and P. Lorenz Bacterial alkaline proteases: Molecular approaches and industrial applications Appl. Microbiol. Biotechnol. 59 2002 15 32
8. L.S. Izotova, A.Y. Strongin, L.N. Chekulaeva, V.E. Sterkin, V.I. Ostoslavskaya, L.A. Lyublinskaya, E.A. Timokhina, and V.M. Stepanov Purification and properties of serine protease from Halobacterium halobium J. Bacteriol. 155 1983 826 830
9. M. Kamekura, and Y. Seno A halophilic extracellular protease from a halophilic archaeabacterium 172 P1 Biochem. Cell. Biol. 68 1990 352 359
10. M. Kamekura, Y. Seno, M.L. Holmes, and M.L. Dyall-Smith Molecular cloning and sequencing of the gene for a halophilic alkaline serine protease (halolysin) from an unidentified halophilic archaea strain (172 P1) and expression of the gene in Haloferax volcanii J. Bacteriol. 174 1992 736 742
11. N. Kato, S. Adachi, K. Tacheuchi, K. Morihara, Y. Tani, and K. Ogata Substrate specificities of the protease from a marine-psychrophilic bacterium, Pseudomonas sp. No. 548 Agric. Biol. Chem. 38 1974 103 109
12. C.T. Kelly, and W.M. Fogarty Microbial alkaline enzymes Process Biochem. 11 1976 3 9
13. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
14. P.L. Manichini, M.G. Fortina, and C. Parini Thermostable alkaline protease produced by Bacillus thermorubber - a new sp. of Bacillus Appl. Microbiol. Biotechnol. 28 1988 409 413
15. K. Morihara Comparative specificity of microbial proteinases Adv. Enzymol. 41 1974 179 243
16. P. Norberg, and B. Hofsten Proteolytic enzymes from extremely halophilic bacteria J. Gen. Microbiol. 55 1969 251 256
17. M.B. Rao, A.M. Tanksale, M.S. Ghatge, and V.V. Deshpande Molecular and biotechnological aspects of microbial proteases Microbiol. Mol. Biol. Rev. 62 1998 597 635
18. K. Ryu, J. Kim, and J.S. Dordick Catalytic properties and potential of an extracellular protease from an extreme halophile Enzyme Microbiol. Technol. 16 1994 266 275
19. S. Sanchez-Porro, S. Martin, E. Mellado, and A. Ventosa Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes J. Appl. Microbiol. 94 2003 295 300
20. C. Sanchez-Porro, E. Mellado, C. Bertoldo, G. Antranikian, and A. Ventosa Screening and characterization of the protease CP1 produced by the moderately halophilic bacterium Pseudoalteromonas sp. strain CP76 Extremophiles 7 2003 221 228
21. V.M. Stepanov, G.N. Rudenskaya, L.P. Revina, Y.B. Gryaznova, E.N. Lysogorskaya, I. Y Filippova, and I.I. Ivanova A serine proteinase of an archaebacterium, Halobacterium mediterraneum: A homologue of eubacterial subtilisins Biochem. J. 285 1992 281 286
22. A.Y.A. Strongin, Z.T. Abramov, N.G. Yroslavtseva, L.A. Baratova, K.A. Shaginyan, L.P. Belianova, and V.M. Stepanov Direct comparison of subtilisin-like intracellular protease of Bacillus licheniformis with the homologous enzymes of Bacillus subtilis J. Bacteriol. 137 1979 1017 1019
23. A. Ventosa, and J.J. Nieto Biotechnological applications and potentialities of halophilic microorganisms World J. Microbiol. Biotechnol. 11 1995 85 94
24. A. Ventosa, J.J. Nieto, and A. Oren Biology of moderately halophilic aerobic bacteria Microbiol. Mol. Biol. Rev. 62 1998 504 544
25. O.P. Ward W.M. Fogarty Microbial Enzymes and Biotechnology 1982 Applied Science Publ. New York 251 371