An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells

BMC Biotechnology

Volumn 10, Issue , 2010, Pages

  Dreyer, Anita M ^a,b   Beauchamp, Jeremy ^c   Matile, Hugues ^c   Pluschke, Gerd ^a,b  

^a SWISS TROPICAL AND PUBLIC HEALTH INSTITUTE   (Switzerland)

^b UNIVERSITY OF BASEL   (Switzerland)

^c F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE PROTEINS; CELL SURFACES; CELL-BASED; CHARACTERISATION; EFFICIENT SYSTEMS; GPI-ANCHORED PROTEIN; MALARIA PARASITE; MAMMALIAN CELLS; MEMBRANE PROTEINS; MEMBRANE-ASSOCIATED PROTEINS; NATIVE CONFORMATION; PROTEIN ANTIGENS; RECOMBINANT PROTEIN; TRANSFECTED CELLS;

ANTIGENS; BIOLOGICAL MEMBRANES; CELL MEMBRANES; IMMUNIZATION; MAMMALS; MONOCLONAL ANTIBODIES; PURIFICATION; SURFACE PLASMON RESONANCE;

PROTEINS;

IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2A; IMMUNOGLOBULIN G2B; IMMUNOGLOBULIN G3; MEMBRANE PROTEIN; MONOCLONAL ANTIBODY; PROTEIN ANTIBODY; RECOMBINANT PROTEIN; PARASITE ANTIGEN; PROTOZOON ANTIBODY;

ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ANTIGEN EXPRESSION; ARTICLE; CELL SURFACE; CONTROLLED STUDY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HYBRIDOMA; IMMUNIZATION; MOUSE; NONHUMAN; OPEN READING FRAME; PLASMODIUM FALCIPARUM; PROTEIN BINDING; PROTEIN PURIFICATION; SEROCONVERSION; SPOROZOITE; ANIMAL; BIOSYNTHESIS; CELL STRAIN HEK293; GENE VECTOR; IMMUNOLOGY;

MAMMALIA; MUS;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODIES, PROTOZOAN; ANTIGENS, PROTOZOAN; GENETIC VECTORS; HEK293 CELLS; HUMANS; HYBRIDOMAS; MEMBRANE PROTEINS; MICE; PLASMODIUM FALCIPARUM; TRANSFECTION;

EID: 78650070290     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-10-87     Document Type: Article

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