Micropatterning as a tool to decipher cell morphogenesis and functions

Journal of Cell Science

Volumn 123, Issue 24, 2010, Pages 4201-4213

  Théry, Manuel ^a  

^a CEA GRENOBLE   (France)

Author keywords

Cell architecture; Cell differentiation; Cell growth; Cell polarity; Microenvironment; Micropatterning

Indexed keywords

ACTIN; INTEGRIN;

ANALYTIC METHOD; CELL ADHESION; CELL CULTURE; CELL DIFFERENTIATION; CELL DIVISION; CELL FUNCTION; CELL GROWTH; CELL MATURATION; CELL MIGRATION; CELL POLARITY; CELL SHAPE; CELL SPREADING; CELL STRUCTURE; CYTOARCHITECTURE; HUMAN; MICROPATTERNING; MICROTUBULE; MORPHOGENESIS; NOTE; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL POLARITY; CELL PROLIFERATION; CELL SHAPE; CELLS; HUMANS;

EID: 78649740028     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: 10.1242/jcs.075150     Document Type: Note

References (142)

1. Arnold, M., Cavalcanti-Adam, E. A., Glass, R., Blummel, J., Eck, W., Kantlehner, M., Kessler, H. and Spatz, J. P. (2004). Activation of integrin function by nanopatterned adhesive interfaces. Chemphyschem 5, 383-388.
2. Azioune, A., Storch, M., Bornens, M., Thery, M. and Piel, M. (2009). Simple and rapid process for single cell micro-patterning. Lab Chip 9, 1640-1642.
3. Azioune, A., Carpi, N., Tseng, Q., Théry, M. and Piel, M. (2010). Protein micropatterns: a direct printing protocol using deep UVs. Methods Cell Biol. 97, 133-146.
4. Balakirev, M. Y., Porte, S., Vernaz-Gris, M., Berger, M., Arie, J. P., Fouque, B. and Chatelain, F. (2005). Photochemical patterning of biological molecules inside a glass capillary. Anal. Chem. 77, 5474-5479. (Pubitemid 41266039)
5. Bauwens, C. L., Peerani, R., Niebruegge, S., Woodhouse, K. A., Kumacheva, E., Husain, M. and Zandstra, P. W. (2008). Control of human embryonic stem cell colony and aggregate size heterogeneity influences differentiation trajectories. Stem Cells 26, 2300-2310.
6. Belisle, J. M., Kunik, D. and Costantino, S. (2009). Rapid multicomponent optical protein patterning. Lab Chip 9, 3580-3585.
7. Beningo, K. A., Lo, C. M. and Wang, Y. L. (2002). Flexible polyacrylamide substrata for the analysis of mechanical interactions at cell-substratum adhesions. Methods Cell Biol. 69, 325-339.
8. Beningo, K. A., Dembo, M. and Wang, Y. L. (2004). Responses of fibroblasts to anchorage of dorsal extracellular matrix receptors. Proc. Natl. Acad. Sci. USA 101, 18024-18029.
9. Bershadsky, A. D., Balaban, N. Q. and Geiger, B. (2003). Adhesion-dependent cell mechanosensitivity. Annu. Rev. Cell Dev. Biol. 19, 677-695.
10. Bhatia, S. N., Balis, U. J., Yarmush, M. L. and Toner, M. (1998). Microfabrication of hepatocyte/fibroblast co-cultures: role of homotypic cell interactions. Biotechnol. Prog. 14, 378-387.
11. Borghi, N., Lowndes, M., Maruthamuthu, V., Gardel, M. L. and Nelson, W. J. (2010). Regulation of cell motile behavior by crosstalk between cadherin- and integrin-mediated adhesions. Proc. Natl. Acad. Sci. USA 107, 13324-13329.
12. Bornens, M. (2008). Organelle positioning and cell polarity. Nat. Rev. Mol. Cell Biol. 9, 874-886.
13. Bray, M. A., Sheehy, S. P. and Parker, K. K. (2008). Sarcomere alignment is regulated by myocyte shape. Cell Motil. Cytoskeleton. 65, 641-651.
14. Brock, A., Chang, E., Ho, C. C., LeDuc, P., Jiang, X., Whitesides, G. M. and Ingber, D. E. (2003). Geometric determinants of directional cell motility revealed using microcontact printing. Langmuir 19, 1611-1617.
15. Carter, S. B. (1967). Haptotactic islands - a method of confining single cells to study individual cell reactions and clone formation. Exp. Cell Res. 48, 189-193.
16. Charest, J. L., Jennings, J. M., King, W. P., Kowalczyk, A. P. and Garcia, A. J. (2009). Cadherin-mediated cell-cell contact regulates keratinocyte differentiation. J. Invest. Dermatol. 129, 564-572.
17. Chen, C. S., Mrksich, M., Huang, S., Whitesides, G. M. and Ingber, D. E. (1997). Geometric control of cell life and death. Science 276, 1425-1428.
18. Connelly, J. T., Gautrot, J. E., Trappmann, B., Tan, D. W., Donati, G., Huck, W. T. and Watt, F. M. (2010). Actin and serum response factor transduce physical cues from the microenvironment to regulate epidermal stem cell fate decisions. Nat. Cell Biol. 12, 711-718.
19. Damljanovic, V., Lagerholm, B. C. and Jacobson, K. (2005). Bulk and micropatterned conjugation of extracellular matrix proteins to characterized polyacrylamide substrates for cell mechanotransduction assays. Biotechniques 39, 847-851.
20. Desai, R. A., Gao, L., Raghavan, S., Liu, W. F. and Chen, C. S. (2009). Cell polarity triggered by cell-cell adhesion via E-cadherin. J. Cell Sci. 122, 905-911.
21. Dike, L. E., Chen, C. S., Mrksich, M., Tien, J., Whitesides, G. M. and Ingber, D. E. (1999). Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates. In Vitro Cell Dev. Biol. Anim. 35, 441-448.
22. Dillmore, W. S., Yousaf, M. N. and Mrksich, M. (2004). A photochemical method for patterning the immobilization of ligands and cells to self-assembled monolayers. Langmuir 20, 7223-7231.
23. Doyle, A. D., Wang, F. W., Matsumoto, K. and Yamada, K. M. (2009). One-dimensional topography underlies three-dimensional fibrillar cell migration. J. Cell Biol. 184, 481-490.
24. Dupin, I., Camand, E. and Etienne-Manneville, S. (2009). Classical cadherins control nucleus and centrosome position and cell polarity. J. Cell Biol. 185, 779-786.
25. Dusseiller, M. R., Schlaepfer, D., Koch, M., Kroschewski, R. and Textor, M. (2005). An inverted microcontact printing method on topographically structured polystyrene chips for arrayed micro-3-D culturing of single cells. Biomaterials 26, 5917-5925. (Pubitemid 40798282)
26. Engler, A. J., Griffin, M. A., Sen, S., Bonnemann, C. G., Sweeney, H. L. and Discher, D. E. (2004). Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments. J. Cell Biol. 166, 877-887.
27. Fan, C. Y., Tung, Y. C., Takayama, S., Meyhofer, E. and Kurabayashi, K. (2008). Electrically programmable surfaces for configurable patterning of cells. Adv. Mater. 20, 1418.
28. Feinberg, A. W., Feigel, A., Shevkoplyas, S. S., Sheehy, S., Whitesides, G. M. and Parker, K. K. (2007). Muscular thin films for building actuators and powering devices. Science 317, 1366-1370.
29. Fink, J., Thery, M., Azioune, A., Dupont, R., Chatelain, F., Bornens, M. and Piel, M. (2007). Comparative study and improvement of current cell micro-patterning techniques. Lab Chip 7, 672-680.
30. Folch, A. and Toner, M. (2000). Microengineering of cellular interactions. Annu. Rev. Biomed. Eng. 2, 227-256.
31. Fraley, S. I., Feng, Y., Krishnamurthy, R., Kim, D. H., Celedon, A., Longmore, G. D. and Wirtz, D. (2010). A distinctive role for focal adhesion proteins in three-dimensional cell motility. Nat. Cell Biol., 12, 598-604.
32. Fu, J., Wang, Y. K., Yang, M. T., Desai, R. A., Yu, X., Liu, Z. and Chen, C. S. (2010). Mechanical regulation of cell function with geometrically modulated elastomeric substrates. Nat. Methods 5, 733-736.
33. Gabi, M., Larmagnac, A., Schulte, P. and Voros, J. (2010). Electrically controlling cell adhesion, growth and migration. Colloids Surf. B Biointerfaces 79, 365-371.
34. Gao, L., McBeath, R. and Chen, C. S. (2010). Stem cell shape regulates a chondrogenic versus myogenic fate through Rac1 and N-cadherin. Stem Cells 28, 564-572.
35. Geisse, N. A., Sheehy, S. P. and Parker, K. K. (2009). Control of myocyte remodeling in vitro with engineered substrates. In Vitro Cell Dev. Biol. Anim. 45, 343-350.
36. Goffin, J. M., Pittet, P., Csucs, G., Lussi, J. W., Meister, J. J. and Hinz, B. (2006). Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers. J. Cell Biol. 172, 259-268. (Pubitemid 43112972)
37. Gomez, E. W., Chen, Q. K., Gjorevski, N. and Nelson, C. M. (2010). Tissue geometry patterns epithelial-mesenchymal transition via intercellular mechanotransduction. J. Cell. Biochem. 110, 44-51.
38. Gray, D. S., Liu, W. F., Shen, C. J., Bhadriraju, K., Nelson, C. M. and Chen, C. S. (2008). Engineering amount of cell-cell contact demonstrates biphasic proliferative regulation through RhoA and the actin cytoskeleton. Exp. Cell Res. 314, 2846-2854.
39. Griffin, M. A., Engler, A. J., Barber, T. A., Healy, K. E., Sweeney, H. L. and Discher, D. E. (2004). Patterning, prestress, and peeling dynamics of myocytes. Biophys. J. 86, 1209-1222.
40. Guillou, H., Depraz-Depland, A., Planus, E., Vianay, B., Chaussy, J., Grichine, A., Albiges-Rizo, C. and Block, M. R. (2008). Lamellipodia nucleation by filopodia depends on integrin occupancy and downstream Rac1 signaling. Exp. Cell Res. 314, 478-488.
41. Guo, Q., Xia, B., Moshiach, S., Xu, C., Jiang, Y., Chen, Y., Sun, Y., Lahti, J. M. and Zhang, X. A. (2008). The microenvironmental determinants for kidney epithelial cyst morphogenesis. Eur. J. Cell Biol. 87, 251-266.
42. Guo, W. H. and Wang, Y. L. (2007). Retrograde fluxes of focal adhesion proteins in response to cell migration and mechanical signals. Mol. Biol. Cell 18, 4519-4527.
43. Gupta, S. K., Meiri, K. F., Mahfooz, K., Bharti, U. and Mani, S. (2010). Coordination between extrinsic extracellular matrix cues and intrinsic responses to orient the centrosome in polarizing cerebellar granule neurons. J. Neurosci. 30, 2755-2766.
44. Hahn, M. S., Miller, J. S. and West, J. L. (2006). Three-dimensional biochemical and biomechanical patterning of hydrogels for guiding cell behavior. Adv. Mater. 18, 2679-2684.
45. Harris, A. (1973). Behavior of cultured cells on substrata of variable adhesiveness. Exp. Cell Res. 77, 285-297.
46. Huang, S., Chen, C. S. and Ingber, D. E. (1998). Control of cyclin D1, p27(Kip1), and cell cycle progression in human capillary endothelial cells by cell shape and cytoskeletal tension. Mol. Biol. Cell 9, 3179-3193.
47. Huang, S., Brangwynne, C. P., Parker, K. K. and Ingber, D. E. (2005). Symmetry-breaking in mammalian cell cohort migration during tissue pattern formation: role of random-walk persistence. Cell Motil. Cytoskeleton 61, 201-213.
48. Hui, E. E. and Bhatia, S. N. (2007). Micromechanical control of cell-cell interactions. Proc. Natl. Acad. Sci. USA 104, 5722-5726.
49. Ingber, D. E. (2003). Tensegrity II. How structural networks influence cellular information processing networks. J. Cell Sci. 116, 1397-1408.
50. Ingber, D. E. (2006). Mechanical control of tissue morphogenesis during embryological development. Int. J. Dev. Biol. 50, 255-266.
51. James, J., Goluch, E. D., Hu, H., Liu, C. and Mrksich, M. (2008). Subcellular curvature at the perimeter of micropatterned cells influences lamellipodial distribution and cell polarity. Cell Motil. Cytoskeleton 65, 841-852.
52. Jiang, X., Bruzewicz, D. A., Wong, A. P., Piel, M. and Whitesides, G. M. (2005). Directing cell migration with asymmetric micropatterns. Proc. Natl. Acad. Sci. USA 102, 975-978.
53. Johnson, K. R., Leight, J. L. and Weaver, V. M. (2007). Demystifying the effects of a three-dimensional microenvironment in tissue morphogenesis. Methods Cell Biol. 83, 547-583.
54. Kam, L., Shain, W., Turner, J. N. and Bizios, R. (2001). Axonal outgrowth of hippocampal neurons on micro-scale networks of polylysine-conjugated laminin. Biomaterials 22, 1049-1054.
55. Khatau, S. B., Hale, C. M., Stewart-Hutchinson, P. J., Patel, M. S., Stewart, C. L., Searson, P. C., Hodzic, D. and Wirtz, D. (2009). A perinuclear actin cap regulates nuclear shape. Proc. Natl. Acad. Sci. USA 106, 19017-19022.
56. Khetani, S. R. and Bhatia, S. N. (2008). Microscale culture of human liver cells for drug development. Nat. Biotechnol. 26, 120-126.
57. Kikuchi, Y., Nakanishi, J., Nakayama, H., Shimizu, T., Yoshino, Y., Yamaguchi, K., Yoshida, Y. and Horiike, Y. (2008a). Grafting Poly(ethylene glycol) to a Glass Surface via a Photocleavable Linker for Light-induced Cell Micropatterning and Cell Proliferation Control. Chemistry Letters, 37, 1062-1063.
58. Kikuchi, Y., Nakanishi, J., Shimizu, T., Nakayama, H., Inoue, S., Yamaguchi, K., Iwai, H., Yoshida, Y., Horiike, Y., Takarada, T. et al. (2008b). Arraying heterotypic single cells on photoactivatable cell-culturing substrates. Langmuir 24, 13084-13095.
59. Kilian, K. A., Bugarija, B., Lahn, B. T. and Mrksich, M. (2010). Geometric cues for directing the differentiation of mesenchymal stem cells. Proc. Natl. Acad. Sci. USA 107, 4872-4877.
60. Kim, J. H., Kushiro, K., Graham, N. A. and Asthagiri, A. R. (2009). Tunable interplay between epidermal growth factor and cell-cell contact governs the spatial dynamics of epithelial growth. Proc. Natl. Acad. Sci. USA 106, 11149-11153.
61. Kim, M., Choi, J. C., Jung, H. R., Katz, J. S., Kim, M. G. and Doh, J. (2010). Addressable micropatterning of multiple proteins and cells by microscope projection photolithography based on a protein friendly photoresist. Langmuir 26, 12112-12118.
62. Kurpinski, K., Chu, J., Hashi, C. and Li, S. (2006). Anisotropic mechanosensing by mesenchymal stem cells. Proc. Natl. Acad. Sci. USA 103, 16095-16100. (Pubitemid 44715157)
63. Kwon, M., Godinho, S. A., Chandhok, N. S., Ganem, N. J., Azioune, A., Thery, M. and Pellman, D. (2008). Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes. Genes Dev. 22, 2189-2203.
64. Ladoux, B., Anon, E., Lambert, M., Rabodzey, A., Hersen, P., Buguin, A., Silberzan, P. and Mege, R. M. (2010). Strength dependence of cadherin-mediated adhesions. Biophys. J. 98, 534-542.
65. Le Beyec, J., Xu, R., Lee, S. Y., Nelson, C. M., Rizki, A., Alcaraz, J. and Bissell, M. J. (2007). Cell shape regulates global histone acetylation in human mammary epithelial cells. Exp. Cell Res. 313, 3066-3075. (Pubitemid 47125878)
66. Lee, G. Y., Kenny, P. A., Lee, E. H. and Bissell, M. J. (2007). Three-dimensional culture models of normal and malignant breast epithelial cells. Nat. Methods 4, 359-365. (Pubitemid 46766981)
67. Lee, S. H., Moon, J. J. and West, J. L. (2008). Three-dimensional micropatterning of bioactive hydrogels via two-photon laser scanning photolithography for guided 3D cell migration. Biomaterials 29, 2962-2968.
68. Lehnert, D., Wehrle-Haller, B., David, C., Weiland, U., Ballestrem, C., Imhof, B. A. and Bastmeyer, M. (2004). Cell behaviour on micropatterned substrata: limits of extracellular matrix geometry for spreading and adhesion. J. Cell Sci. 117, 41-52.
69. Lelievre, S. A., Weaver, V. M., Nickerson, J. A., Larabell, C. A., Bhaumik, A., Petersen, O. W. and Bissell, M. J. (1998). Tissue phenotype depends on reciprocal interactions between the extracellular matrix and the structural organization of the nucleus. Proc. Natl. Acad. Sci. USA 95, 14711-14716.
70. Levina, E. M., Kharitonova, M. A., Rovensky, Y. A. and Vasiliev, J. M. (2001). Cytoskeletal control of fibroblast length: experiments with linear strips of substrate. J. Cell Sci. 114, 4335-4341.
71. Li, B., Li, F., Puskar, K. M. and Wang, J. H. (2009). Spatial patterning of cell proliferation and differentiation depends on mechanical stress magnitude. J. Biomech. 42, 1622-1627.
72. Liu Tsang, V., Chen, A. A., Cho, L. M., Jadin, K. D., Sah, R. L., DeLong, S., West, J. L. and Bhatia, S. N. (2007). Fabrication of 3D hepatic tissues by additive photopatterning of cellular hydrogels. FASEB J. 21, 790-801.
73. Liu, Y., Mu, L., Liu, B. H. and Kong, J. L. (2005a). Controlled switchable surface. Chem. Eur. J. 11, 2622-2631.
74. Liu, Y., Sun, S., Singha, S., Cho, M. R. and Gordon, R. J. (2005b). 3D femtosecond laser patterning of collagen for directed cell attachment. Biomaterials 26, 4597-4605.
75. Liu, Z., Tan, J. L., Cohen, D. M., Yang, M. T., Sniadecki, N. J., Ruiz, S. A., Nelson, C. M. and Chen, C. S. (2010). Mechanical tugging force regulates the size of cell-cell junctions. Proc. Natl. Acad. Sci. USA 107, 9944-9949.
76. Mahmud, G., Campbell, C. J., Bishop, K. J. M., Komarova, Y. A., Chaga, O., Soh, S., Huda, S., Kandere-Grzybowska, K. and Grzybowski, B. A. (2009). Directing cell motions on micropatterned ratchets. Nat. Phys. 5, 606-612.
77. Mammoto, A., Huang, S., Moore, K., Oh, P. and Ingber, D. E. (2004). Role of RhoA, mDia, and ROCK in cell shape-dependent control of the Skp2-p27kip1 pathway and the G1/S transition. J. Biol. Chem. 279, 26323-26330.
78. McBeath, R., Pirone, D. M., Nelson, C. M., Bhadriraju, K. and Chen, C. S. (2004). Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev. Cell 6, 483-495.
79. Moon, J. J., Hahn, M. S., Kim, I., Nsiah, B. A. and West, J. L. (2009). Micropatterning of poly(ethylene glycol) diacrylate hydrogels with biomolecules to regulate and guide endothelial morphogenesis. Tissue Eng. Part A 15, 579-585.
80. Mrksich, M. (2009). Using self-assembled monolayers to model the extracellular matrix. Acta Biomater. 5, 832-841.
81. Nakanishi, J., Kikuchi, Y., Takarada, T., Nakayama, H., Yamaguchi, K. and Maeda, M. (2006). Spatiotemporal control of cell adhesion on a self-assembled monolayer having a photocleavable protecting group. Anal. Chim. Acta 578, 100-104. (Pubitemid 44373822)
82. Nakanishi, J., Takarada, T., Yamaguchi, K. and Maeda, M. (2008). Recent advances in cell micropatterning techniques for bioanalytical and biomedical sciences. Anal. Sci. 24, 67-72.
83. Nelson, C. M. and Chen, C. S. (2002). Cell-cell signaling by direct contact increases cell proliferation via a PI3K-dependent signal. FEBS Lett. 514, 238-242.
84. Nelson, C. M., Jean, R. P., Tan, J. L., Liu, W. F., Sniadecki, N. J., Spector, A. A. and Chen, C. S. (2005). Emergent patterns of growth controlled by multicellular form and mechanics. Proc. Natl. Acad. Sci. USA 102, 11594-11599.
85. Nelson, C. M., Vanduijn, M. M., Inman, J. L., Fletcher, D. A. and Bissell, M. J. (2006). Tissue geometry determines sites of mammary branching morphogenesis in organotypic cultures. Science 314, 298-300.
86. Nelson, C. M., Khauv, D., Bissell, M. J. and Radisky, D. C. (2008). Change in cell shape is required for matrix metalloproteinase-induced epithelial-mesenchymal transition of mammary epithelial cells. J. Cell. Biochem. 105, 25-33.
87. O'Brien, L. E., Zegers, M. M. and Mostov, K. E. (2002). Opinion: Building epithelial architecture: insights from three-dimensional culture models. Nat. Rev. Mol. Cell Biol. 3, 531-537.
88. Ochsner, M., Textor, M., Vogel, V. and Smith, M. L. (2010). Dimensionality controls cytoskeleton assembly and metabolism of fibroblast cells in response to rigidity and shape. PLoS ONE 5, e9445.
89. Okochi, N., Okazaki, T. and Hattori, H. (2009). Encouraging effect of cadherin-mediated cell-cell junctions on transfer printing of micropatterned vascular endothelial cells. Langmuir 25, 6947-6953.
90. Oliva, A. A., Jr, James, C. D., Kingman, C. E., Craighead, H. G. and Banker, G. A. (2003). Patterning axonal guidance molecules using a novel strategy for microcontact printing. Neurochem. Res. 28, 1639-1648.
91. Ouyang, M., Sun, J., Chien, S. and Wang, Y. (2008). Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors. Proc. Natl. Acad. Sci. USA 105, 14353-14358.
92. Parker, K. K., Brock, A. L., Brangwynne, C., Mannix, R. J., Wang, N., Ostuni, E., Geisse, N. A., Adams, J. C., Whitesides, G. M. and Ingber, D. E. (2002). Directional control of lamellipodia extension by constraining cell shape and orienting cell tractional forces. FASEB J. 16, 1195-1204.
93. Peerani, R., Rao, B. M., Bauwens, C., Yin, T., Wood, G. A., Nagy, A., Kumacheva, E. and Zandstra, P. W. (2007). Niche-mediated control of human embryonic stem cell self-renewal and differentiation. EMBO J. 26, 4744-4755.
94. Petitjean, L., Reffay, M., Grasland-Mongrain, E., Poujade, M., Ladoux, B., Buguin, A. and Silberzan, P. (2010). Velocity fields in a collectively migrating epithelium. Biophys. J. 98, 1790-1800.
95. Pitaval, A., Tseng, Q., Bornens, M. and Théry, M. (2010). Cell shape and contractility regulate ciliogenesis in cell cycle arrested cells. J. Cell Biol. 191, 303-312.
96. Polte, T. R., Eichler, G. S., Wang, N. and Ingber, D. E. (2004). Extracellular matrix controls myosin light chain phosphorylation and cell contractility through modulation of cell shape and cytoskeletal prestress. Am. J. Physiol. Cell Physiol. 286, C518-C528.
97. Poujade, M., Grasland-Mongrain, E., Hertzog, A., Jouanneau, J., Chavrier, P., Ladoux, B., Buguin, A. and Silberzan, P. (2007). Collective migration of an epithelial monolayer in response to a model wound. Proc. Natl. Acad. Sci. USA 104, 15988-15993. (Pubitemid 350099346)
98. Pouthas, F., Girard, P., Lecaudey, V., Ly, T. B., Gilmour, D., Boulin, C., Pepperkok, R. and Reynaud, E. G. (2008). In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum. J. Cell Sci. 121, 2406-2414.
99. Raghavan, S., Desai, R. A., Kwon, Y., Mrksich, M. and Chen, C. S. (2010a). Micropatterned dynamically adhesive substrates for cell migration. Langmuir 26, 17733-17738.
100. Raghavan, S., Nelson, C. M., Baranski, J. D., Lim, E. and Chen, C. S. (2010b). Geometrically controlled endothelial tubulogenesis in micropatterned gels. Tissue Eng Part A 16, 2255-2263.
101. Roca-Cusachs, P., Alcaraz, J., Sunyer, R., Samitier, J., Farre, R. and Navajas, D. (2008). Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation. Biophys. J. 94, 4984-4995.
102. Rossier, O. M., Gauthier, N., Biais, N., Vonnegut, W., Fardin, M. A., Avigan, P., Heller, E. R., Mathur, A., Ghassemi, S., Koeckert, M. S. et al. (2010). Force generated by actomyosin contraction builds bridges between adhesive contacts. EMBO J. 29, 1055-1068.
103. Ruiz, S. A. and Chen, C. S. (2007). Microcontact printing: a tool to pattern. Soft Matter 3, 168-177.
104. Ruiz, S. A. and Chen, C. S. (2008). Emergence of patterned stem cell differentiation within multicellular structures. Stem Cells 26, 2921-2927.
105. Saez, A., Ghibaudo, M., Buguin, A., Silberzan, P. and Ladoux, B. (2007). Rigidity-driven growth and migration of epithelial cells on microstructured anisotropic substrates. Proc. Natl. Acad. Sci. USA 104, 8281-8286. (Pubitemid 47175479)
106. Schauer, K., Duong, T., Bleakley, K., Bardin, S., Bornens, M. and Goud, B. (2010). Probabilistic density maps to study global endomembrane organization. Nat. Methods 7, 560-566.
107. Shah, S., Lee, J. Y., Verkhoturov, S., Tuleuova, N., Schweikert, E. A., Ramanculov, E. and Revzin, A. (2008). Exercising spatiotemporal control of cell attachment with optically transparent microelectrodes. Langmuir 24, 6837-6844.
108. Shelly, M., Lim, B. K., Cancedda, L., Heilshorn, S. C., Gao, H. and Poo, M. M. (2010). Local and long-range reciprocal regulation of cAMP and cGMP in axon/dendrite formation. Science 327, 547-552.
109. Shi, P., Shen, K. and Kam, L. C. (2007). Local presentation of L1 and N-cadherin in multicomponent, microscale patterns differentially direct neuron function in vitro. Dev. Neurobiol. 67, 1765-1776.
110. Singhvi, R., Kumar, A., Lopez, G. P., Stephanopoulos, G. N., Wang, D. I., Whitesides, G. M. and Ingber, D. E. (1994). Engineering cell shape and function. Science 264, 696-698.
111. Steinberg, M. S. (2007). Differential adhesion in morphogenesis: a modern view. Curr. Opin. Genet. Dev. 17, 281-286.
112. Stenger, D. A., Hickman, J. J., Bateman, K. E., Ravenscroft, M. S., Ma, W., Pancrazio, J. J., Shaffer, K., Schaffner, A. E., Cribbs, D. H. and Cotman, C. W. (1998). Microlithographic determination of axonal/dendritic polarity in cultured hippocampal neurons. J. Neurosci. Methods 82, 167-173.
113. Su, J., Brau, R. R., Jiang, X., Whitesides, G. M., Lange, M. J. and So, P. T. (2007). Geometric confinement influences cellular mechanical properties II - intracellular variances in polarized cells. Mol. Cell. Biomech. 4, 105-118.
114. Szabo, B., Kornyei, Z., Zach, J., Selmeczi, D., Csucs, G., Czirok, A. and Vicsek, T. (2004). Auto-reverse nuclear migration in bipolar mammalian cells on micropatterned surfaces. Cell Motil. Cytoskeleton 59, 38-49. (Pubitemid 39167097)
115. Tan, J. L., Tien, J., Pirone, D. M., Gray, D. S., Bhadriraju, K. and Chen, C. S. (2003). Cells lying on a bed of microneedles: an approach to isolate mechanical force. Proc. Natl. Acad. Sci. USA 100, 1484-1489. (Pubitemid 36254472)
116. Tay, C. Y., Yu, H., Pal, M., Leong, W. S., Tan, N. S., Ng, K. W., Leong, D. T. and Tan, L. P. (2010). Micropatterned matrix directs differentiation of human mesenchymal stem cells towards myocardial lineage. Exp. Cell Res. 316, 1159-1168.
117. Thakar, R. G., Ho, F., Huang, N. F., Liepmann, D. and Li, S. (2003). Regulation of vascular smooth muscle cells by micropatterning. Biochem. Biophys. Res. Commun. 307, 883-890.
118. Thakar, R. G., Cheng, Q., Patel, S., Chu, J., Nasir, M., Liepmann, D., Komvopoulos, K. and Li, S. (2009). Cell-shape regulation of smooth muscle cell proliferation. Biophys. J. 96, 3423-3432.
119. Théry, M. and Bornens, M. (2006). Cell shape and cell division. Curr. Opin. Cell Biol. 18, 648-657.
120. Théry, M. and Piel, M. (2009). Adhesive micropatterns for cells: a microcontact printing protocol. Cold Spring Harbor Protoc. 2009, pdb.prot5255.
121. Théry, M., Racine, V., Pepin, A., Piel, M., Chen, Y., Sibarita, J. B. and Bornens, M. (2005). The extracellular matrix guides the orientation of the cell division axis. Nat. Cell Biol. 7, 947-953.
122. Théry, M., Pepin, A., Dressaire, E., Chen, Y. and Bornens, M. (2006a). Cell distribution of stress fibres in response to the geometry of the adhesive environment. Cell Motil. Cytoskeleton 63, 341-355.
123. Théry, M., Racine, V., Piel, M., Pepin, A., Dimitrov, A., Chen, Y., Sibarita, J. B. and Bornens, M. (2006b). Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity. Proc. Natl. Acad. Sci. USA 103, 19771-19776.
124. Théry, M., Jimenez-Dalmaroni, A., Racine, V., Bornens, M. and Julicher, F. (2007). Experimental and theoretical study of mitotic spindle orientation. Nature 447, 493-496. (Pubitemid 46816737)
125. Trepat, X., Wasserman, M. R., Angelini, T. E., Millet, E., Weitz, D. A., Butler, J. P. and Fredberg, J. J. (2009). Physical forces during collective cell migration. Nat. Phys. 5, 426-430.
126. Tsai, J. and Kam, L. (2009). Rigidity-dependent cross talk between integrin and cadherin signaling. Biophys. J. 96, L39-L41.
127. Vartanian, K. B., Berny, M. A., McCarty, O. J., Hanson, S. R. and Hinds, M. T. (2010). Cytoskeletal structure regulates endothelial cell immunogenicity independent of fluid shear stress. Am. J. Physiol. Cell Physiol. 298, C333-C341.
128. Vartiainen, M. K., Guettler, S., Larijani, B. and Treisman, R. (2007). Nuclear actin regulates dynamic subcellular localization and activity of the SRF cofactor MAL. Science 316, 1749-1752. (Pubitemid 46988550)
129. Vogt, A. K., Stefani, F. D., Best, A., Nelles, G., Yasuda, A., Knoll, W. and Offenhausser, A. (2004). Impact of micropatterned surfaces on neuronal polarity. J. Neurosci. Methods 134, 191-198. (Pubitemid 38299245)
130. Wang, N., Ostuni, E., Whitesides, G. M. and Ingber, D. E. (2002). Micropatterning tractional forces in living cells. Cell Motil. Cytoskeleton 52, 97-106.
131. Wang, N., Tytell, J. D. and Ingber, D. E. (2009). Mechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus. Nat. Rev. Mol. Cell Biol. 10, 75-82.
132. Watt, F. M., Jordan, P. W. and O'Neill, C. H. (1988). Cell shape controls terminal differentiation of human epidermal keratinocytes. Proc. Natl. Acad. Sci. USA 85, 5576-5580.
133. Welle, A. and Gottwald, E. (2002). UV-based patterning of polymeric substrates for cell culture applications. Biomed. Microdevices 4, 33-41. (Pubitemid 34240362)
134. Welle, A., Horn, S., Schimmelpfeng, J. and Kalka, D. (2005). Photo-chemically patterned polymer surfaces for controlled PC-12 adhesion and neurite guidance. J. Neurosci. Methods 142, 243-250. (Pubitemid 40215458)
135. Wheeler, B. C., Corey, J. M., Brewer, G. J. and Branch, D. W. (1999). Microcontact printing for precise control of nerve cell growth in culture. J. Biomech. Eng. 121, 73-78.
136. Whitesides, G. M., Ostuni, E., Takayama, S., Jiang, X. and Ingber, D. E. (2001). Soft lithography in biology and biochemistry. Annu. Rev. Biomed. Eng. 3, 335-373.
137. Wu, C. C., Li, Y. S., Haga, J. H., Kaunas, R., Chiu, J. J., Su, F. C., Usami, S. and Chien, S. (2007). Directional shear flow and Rho activation prevent the endothelial cell apoptosis induced by micropatterned anisotropic geometry. Proc. Natl. Acad. Sci. USA 104, 1254-1259.
138. Xia, N., Thodeti, C. K., Hunt, T. P., Xu, Q., Ho, M., Whitesides, G. M., Westervelt, R. and Ingber, D. E. (2008). Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation. FASEB J. 22, 1649-1659.
139. Yeo, W. S. and Mrksich, M. (2006). Electroactive self-assembled monolayers that permit orthogonal control over the adhesion of cells to patterned substrates. Langmuir 22, 10816-10820.
140. Yeo, W. S., Yousaf, M. N. and Mrksich, M. (2003). Dynamic interfaces between cells and surfaces: electroactive substrates that sequentially release and attach cells. J. Am. Chem. Soc. 125, 14994-14995.
141. Yousaf, M. N., Houseman, B. T. and Mrksich, M. (2001). Using electroactive substrates to pattern the attachment of two different cell populations. Proc. Natl. Acad. Sci. USA 98, 5992-5996.
142. Zhu, J., Burakov, A., Rodionov, V. and Mogilner, A. (2010). Finding the Cell Center by a Balance of Dynein and Myosin Pulling and Microtubule Pushing: a Computational Study. Mol. Biol. Cell., Epub ahead of print. PMID: 20980619.