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Volumn 53, Issue 9, 2010, Pages 1049-1056

Electron microscopic visualization of asymmetric precursor translocation intermediates: SecA functions as a dimer

Author keywords

electron microscopy; SecA; SecYEG; single particle analysis

Indexed keywords

ADENOSINE TRIPHOSPHATASE; BACTERIAL PROTEIN; CARRIER PROTEIN; ESCHERICHIA COLI PROTEIN; PROTEIN PRECURSOR; PROTEOLIPID; PROTEOLIPOSOMES; SECA PROTEIN, BACTERIA; SECYEG PROTEIN, E COLI;

EID: 78649543432     PISSN: 16747305     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11427-010-4061-x     Document Type: Article
Times cited : (6)

References (42)
  • 1
    • 36749001066 scopus 로고    scopus 로고
    • Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes
    • Rapoport T A. Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes. Nature, 2007, 450: 663-669.
    • (2007) Nature , vol.450 , pp. 663-669
    • Rapoport, T.A.1
  • 2
    • 0031037648 scopus 로고    scopus 로고
    • Binding of SecB to ribosome-bound polypeptides has the same characteristics as binding to full-length, denatured proteins
    • Randall L L, Topping T B, Hardy S J, et al. Binding of SecB to ribosome-bound polypeptides has the same characteristics as binding to full-length, denatured proteins. Proc Natl Acad Sci USA, 1997, 94: 802-807.
    • (1997) Proc Natl Acad Sci USA , vol.94 , pp. 802-807
    • Randall, L.L.1    Topping, T.B.2    Hardy, S.J.3
  • 3
    • 0028064967 scopus 로고
    • SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion
    • Economou A, Wickner W. SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion. Cell, 1994, 78: 835-843.
    • (1994) Cell , vol.78 , pp. 835-843
    • Economou, A.1    Wickner, W.2
  • 4
    • 0026073817 scopus 로고
    • + and ATP function at different steps of the catalytic cycle of preprotein translocase
    • + and ATP function at different steps of the catalytic cycle of preprotein translocase. Cell, 1991, 64: 927-939.
    • (1991) Cell , vol.64 , pp. 927-939
    • Schiebel, E.1    Driessen, A.J.2    Hartl, F.U.3
  • 5
    • 0035374515 scopus 로고    scopus 로고
    • Functional signal peptides bind a soluble N-terminal fragment of SecA and inhibit its ATPase activity
    • Triplett T L, Sgrignoli A R, Gao F B, et al. Functional signal peptides bind a soluble N-terminal fragment of SecA and inhibit its ATPase activity. J Biol Chem, 2001, 276: 19648-19655.
    • (2001) J Biol Chem , vol.276 , pp. 19648-19655
    • Triplett, T.L.1    Sgrignoli, A.R.2    Gao, F.B.3
  • 6
    • 0037134449 scopus 로고    scopus 로고
    • Allosteric communication between signal peptides and the SecA protein DEAD motor ATPase domain
    • Baud C, Karamanou S, Sianidis G, et al. Allosteric communication between signal peptides and the SecA protein DEAD motor ATPase domain. J Biol Chem, 2002, 277: 13724-13731.
    • (2002) J Biol Chem , vol.277 , pp. 13724-13731
    • Baud, C.1    Karamanou, S.2    Sianidis, G.3
  • 7
    • 0036663201 scopus 로고    scopus 로고
    • Bacterial secretome: the assembly manual and operating instructions (Review)
    • Economou A. Bacterial secretome: the assembly manual and operating instructions (Review). Mol Membr Biol, 2002, 19: 159-169.
    • (2002) Mol Membr Biol , vol.19 , pp. 159-169
    • Economou, A.1
  • 8
    • 15744404686 scopus 로고    scopus 로고
    • The bacterial ATPase SecA functions as a monomer in protein translocation
    • Or E, Boyd D, Gon S, et al. The bacterial ATPase SecA functions as a monomer in protein translocation. J Biol Chem, 2005, 280: 9097-9105.
    • (2005) J Biol Chem , vol.280 , pp. 9097-9105
    • Or, E.1    Boyd, D.2    Gon, S.3
  • 9
    • 0037009514 scopus 로고    scopus 로고
    • Dissociation of the dimeric SecA ATPase during protein translocation across the bacterial membrane
    • Or E, Navon A, Rapoport T. Dissociation of the dimeric SecA ATPase during protein translocation across the bacterial membrane. Embo J, 2002, 21: 4470-4479.
    • (2002) Embo J , vol.21 , pp. 4470-4479
    • Or, E.1    Navon, A.2    Rapoport, T.3
  • 10
    • 27444439568 scopus 로고    scopus 로고
    • Covalently dimerized SecA is functional in protein translocation
    • de Keyzer J, van der Sluis E O, Spelbrink R E, et al. Covalently dimerized SecA is functional in protein translocation. J Biol Chem, 2005, 280: 35255-35260.
    • (2005) J Biol Chem , vol.280 , pp. 35255-35260
    • de Keyzer, J.1    van der Sluis, E.O.2    Spelbrink, R.E.3
  • 11
    • 19644372742 scopus 로고    scopus 로고
    • Dimeric SecA is essential for protein translocation
    • Jilaveanu L B, Zito C R, Oliver D. Dimeric SecA is essential for protein translocation. Proc Natl Acad Sci USA, 2005, 102: 7511-7516.
    • (2005) Proc Natl Acad Sci USA , vol.102 , pp. 7511-7516
    • Jilaveanu, L.B.1    Zito, C.R.2    Oliver, D.3
  • 12
    • 38949141646 scopus 로고    scopus 로고
    • Additional in vitro and in vivo evidence for SecA functioning as dimers in the membrane: dissociation into monomers is not essential for protein translocation in Escherichia coli
    • Wang H, Na B, Yang H, et al. Additional in vitro and in vivo evidence for SecA functioning as dimers in the membrane: dissociation into monomers is not essential for protein translocation in Escherichia coli. J Bacteriol, 2008, 190: 1413-1418.
    • (2008) J Bacteriol , vol.190 , pp. 1413-1418
    • Wang, H.1    Na, B.2    Yang, H.3
  • 13
    • 55749113726 scopus 로고    scopus 로고
    • Reexamination of the role of the amino terminus of SecA in promoting its dimerization and functional state
    • Das S, Stivison E, Folta-Stogniew E, et al. Reexamination of the role of the amino terminus of SecA in promoting its dimerization and functional state. J Bacteriol, 2008, 190: 7302-7307.
    • (2008) J Bacteriol , vol.190 , pp. 7302-7307
    • Das, S.1    Stivison, E.2    Folta-Stogniew, E.3
  • 14
    • 35348908966 scopus 로고    scopus 로고
    • Bacterial protein secretion through the translocase nanomachine
    • Papanikou E, Karamanou S, Economou A. Bacterial protein secretion through the translocase nanomachine. Nat Rev Microbiol, 2007, 5: 839-851.
    • (2007) Nat Rev Microbiol , vol.5 , pp. 839-851
    • Papanikou, E.1    Karamanou, S.2    Economou, A.3
  • 15
    • 57649143096 scopus 로고    scopus 로고
    • Full-length Escherichia coli SecA dimerizes in a closed conformation in solution as determined by cryo-electron microscopy
    • Chen Y, Pan X, Tang Y, et al. Full-length Escherichia coli SecA dimerizes in a closed conformation in solution as determined by cryo-electron microscopy. J Biol Chem, 2008, 283: 28783-28787.
    • (2008) J Biol Chem , vol.283 , pp. 28783-28787
    • Chen, Y.1    Pan, X.2    Tang, Y.3
  • 16
    • 0345196631 scopus 로고    scopus 로고
    • The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex
    • Meyer T H, Menetret J F, Breitling R, et al. The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex. J Mol Biol, 1999, 285: 1789-1800.
    • (1999) J Mol Biol , vol.285 , pp. 1789-1800
    • Meyer, T.H.1    Menetret, J.F.2    Breitling, R.3
  • 17
    • 27644518797 scopus 로고    scopus 로고
    • The oligomeric distribution of SecYEG is altered by SecA and translocation ligands
    • Scheuring J, Braun N, Nothdurft L, et al. The oligomeric distribution of SecYEG is altered by SecA and translocation ligands. J Mol Biol, 2005, 354: 258-271.
    • (2005) J Mol Biol , vol.354 , pp. 258-271
    • Scheuring, J.1    Braun, N.2    Nothdurft, L.3
  • 18
    • 33947717366 scopus 로고    scopus 로고
    • Protein translocation is mediated by oligomers of the SecY complex with one SecY copy forming the channel
    • Osborne A R, Rapoport T A. Protein translocation is mediated by oligomers of the SecY complex with one SecY copy forming the channel. Cell, 2007, 129: 97-110.
    • (2007) Cell , vol.129 , pp. 97-110
    • Osborne, A.R.1    Rapoport, T.A.2
  • 19
    • 0025005885 scopus 로고
    • Translocation of ProOmpA possessing an intramolecular disulfide bridge into membrane vesicles of Escherichia coli. Effect of membrane energization
    • Tani K, Tokuda H, Mizushima S. Translocation of ProOmpA possessing an intramolecular disulfide bridge into membrane vesicles of Escherichia coli. Effect of membrane energization. J Biol Chem, 1990, 265: 17341-17347.
    • (1990) J Biol Chem , vol.265 , pp. 17341-17347
    • Tani, K.1    Tokuda, H.2    Mizushima, S.3
  • 20
    • 0034161573 scopus 로고    scopus 로고
    • SecYEG assembles into a tetramer to form the active protein translocation channel
    • Manting E H, van Der Does C, Remigy H, et al. SecYEG assembles into a tetramer to form the active protein translocation channel. Embo J, 2000, 19: 852-861.
    • (2000) Embo J , vol.19 , pp. 852-861
    • Manting, E.H.1    van der Does, C.2    Remigy, H.3
  • 21
    • 0034663803 scopus 로고    scopus 로고
    • Evaluating the oligomeric state of SecYEG in preprotein translocase
    • Yahr T L, Wickner W T. Evaluating the oligomeric state of SecYEG in preprotein translocase. Embo J, 2000, 19: 4393-4401.
    • (2000) Embo J , vol.19 , pp. 4393-4401
    • Yahr, T.L.1    Wickner, W.T.2
  • 22
    • 0036500974 scopus 로고    scopus 로고
    • The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure
    • Bessonneau P, Besson V, Collinson I, et al. The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure. Embo J, 2002, 21: 995-1003.
    • (2002) Embo J , vol.21 , pp. 995-1003
    • Bessonneau, P.1    Besson, V.2    Collinson, I.3
  • 23
    • 0035873223 scopus 로고    scopus 로고
    • Projection structure and oligomeric properties of a bacterial core protein translocase
    • Collinson I, Breyton C, Duong F, et al. Projection structure and oligomeric properties of a bacterial core protein translocase. Embo J, 2001, 20: 2462-2471.
    • (2001) Embo J , vol.20 , pp. 2462-2471
    • Collinson, I.1    Breyton, C.2    Duong, F.3
  • 24
    • 54049111011 scopus 로고    scopus 로고
    • Structure of a complex of the ATPase SecA and the protein-translocation channel
    • Zimmer J, Nam Y, Rapoport T A. Structure of a complex of the ATPase SecA and the protein-translocation channel. Nature, 2008, 455: 936-943.
    • (2008) Nature , vol.455 , pp. 936-943
    • Zimmer, J.1    Nam, Y.2    Rapoport, T.A.3
  • 25
    • 0037389586 scopus 로고    scopus 로고
    • Ring-like pore structures of SecA: implication for bacterial protein-conducting channels
    • Wang H W, Chen Y, Yang H, et al. Ring-like pore structures of SecA: implication for bacterial protein-conducting channels. Proc Natl Acad Sci USA, 2003, 100: 4221-4226.
    • (2003) Proc Natl Acad Sci USA , vol.100 , pp. 4221-4226
    • Wang, H.W.1    Chen, Y.2    Yang, H.3
  • 26
    • 0029997381 scopus 로고    scopus 로고
    • Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation
    • Nishiyama K, Suzuki T, Tokuda H. Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation. Cell, 1996, 85: 71-81.
    • (1996) Cell , vol.85 , pp. 71-81
    • Nishiyama, K.1    Suzuki, T.2    Tokuda, H.3
  • 27
    • 0029919178 scopus 로고    scopus 로고
    • 2+ at an early post-targeting stage of translocation
    • 2+ at an early post-targeting stage of translocation. J Biol Chem, 1996, 271: 9429-9436.
    • (1996) J Biol Chem , vol.271 , pp. 9429-9436
    • Yoshihisa, T.1    Ito, K.2
  • 28
    • 0242317917 scopus 로고    scopus 로고
    • Reconstitution of purified bacterial preprotein translocase in liposomes
    • van der Does C, de Keyzer J, van der Laan M, et al. Reconstitution of purified bacterial preprotein translocase in liposomes. Methods Enzymol, 2003, 372: 86-98.
    • (2003) Methods Enzymol , vol.372 , pp. 86-98
    • van der Does, C.1    de Keyzer, J.2    van der Laan, M.3
  • 29
    • 0030897086 scopus 로고    scopus 로고
    • Identification of a region of interaction between Escherichia coli SecA and SecY proteins
    • Snyders S, Ramamurthy V, Oliver D. Identification of a region of interaction between Escherichia coli SecA and SecY proteins. J Biol Chem, 1997, 272: 11302-11306.
    • (1997) J Biol Chem , vol.272 , pp. 11302-11306
    • Snyders, S.1    Ramamurthy, V.2    Oliver, D.3
  • 30
    • 0034861414 scopus 로고    scopus 로고
    • Immunogold localisation of P-glycoprotein in supported lipid bilayers by transmission electron microscopy and atomic force microscopy
    • Ruspantini I, Diociaiuti M, Ippoliti R, et al. Immunogold localisation of P-glycoprotein in supported lipid bilayers by transmission electron microscopy and atomic force microscopy. Histochem J, 2001, 33: 305-309.
    • (2001) Histochem J , vol.33 , pp. 305-309
    • Ruspantini, I.1    Diociaiuti, M.2    Ippoliti, R.3
  • 31
    • 0031435335 scopus 로고    scopus 로고
    • The catalytic cycle of the Escherichia coli SecA ATPase comprises two distinct preprotein translocation events
    • van der Wolk J P, de Wit J G, Driessen A J. The catalytic cycle of the Escherichia coli SecA ATPase comprises two distinct preprotein translocation events. Embo J, 1997, 16: 7297-7304.
    • (1997) Embo J , vol.16 , pp. 7297-7304
    • van der Wolk, J.P.1    de Wit, J.G.2    Driessen, A.J.3
  • 32
    • 0033377664 scopus 로고    scopus 로고
    • EMAN: semiautomated software for high-resolution single-particle reconstructions
    • Ludtke S J, Baldwin P R, Chiu W. EMAN: semiautomated software for high-resolution single-particle reconstructions. J Struct Biol, 1999, 128: 82-97.
    • (1999) J Struct Biol , vol.128 , pp. 82-97
    • Ludtke, S.J.1    Baldwin, P.R.2    Chiu, W.3
  • 33
    • 0029975088 scopus 로고    scopus 로고
    • SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields
    • Frank J, Radermacher M, Penczek P, et al. SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields. J Struct Biol, 1996, 116: 190-199.
    • (1996) J Struct Biol , vol.116 , pp. 190-199
    • Frank, J.1    Radermacher, M.2    Penczek, P.3
  • 35
    • 0028142465 scopus 로고
    • Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY, SecE, and SecG (p12)
    • Hanada M, Nishiyama K I, Mizushima S, et al. Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY, SecE, and SecG (p12). J Biol Chem, 1994, 269: 23625-23631.
    • (1994) J Biol Chem , vol.269 , pp. 23625-23631
    • Hanada, M.1    Nishiyama, K.I.2    Mizushima, S.3
  • 36
    • 0025036708 scopus 로고
    • The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane
    • Hartl F U, Lecker S, Schiebel E, et al. The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane. Cell, 1990, 63: 269-279.
    • (1990) Cell , vol.63 , pp. 269-279
    • Hartl, F.U.1    Lecker, S.2    Schiebel, E.3
  • 37
    • 0025087853 scopus 로고
    • The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation
    • Brundage L, Hendrick J P, Schiebel E, et al. The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation. Cell, 1990, 62: 649-657.
    • (1990) Cell , vol.62 , pp. 649-657
    • Brundage, L.1    Hendrick, J.P.2    Schiebel, E.3
  • 38
    • 0027503249 scopus 로고
    • Purified Escherichia coli preprotein translocase catalyzes multiple cycles of precursor protein translocation
    • Bassilana M, Wickner W. Purified Escherichia coli preprotein translocase catalyzes multiple cycles of precursor protein translocation. Biochemistry, 1993, 32: 2626-2630.
    • (1993) Biochemistry , vol.32 , pp. 2626-2630
    • Bassilana, M.1    Wickner, W.2
  • 39
    • 0029148784 scopus 로고
    • SecYEG and SecA are the stoichiometric components of preprotein translocase
    • Douville K, Price A, Eichler J, et al. SecYEG and SecA are the stoichiometric components of preprotein translocase. J Biol Chem, 1995, 270: 20106-20111.
    • (1995) J Biol Chem , vol.270 , pp. 20106-20111
    • Douville, K.1    Price, A.2    Eichler, J.3
  • 40
    • 50649104037 scopus 로고    scopus 로고
    • Protein translocation across the bacterial cytoplasmic membrane
    • Driessen A J, Nouwen N. Protein translocation across the bacterial cytoplasmic membrane. Annu Rev Biochem, 2008, 77: 643-667.
    • (2008) Annu Rev Biochem , vol.77 , pp. 643-667
    • Driessen, A.J.1    Nouwen, N.2
  • 41
    • 0347192985 scopus 로고    scopus 로고
    • X-ray structure of a protein-conducting channel
    • van den Berg B, Clemons W M Jr, Collinson I, et al. X-ray structure of a protein-conducting channel. Nature, 2004, 427: 36-44.
    • (2004) Nature , vol.427 , pp. 36-44
    • van den Berg, B.1    Clemons Jr., W.M.2    Collinson, I.3
  • 42
    • 0041736710 scopus 로고    scopus 로고
    • Binding, activation and dissociation of the dimeric SecA ATPase at the dimeric SecYEG translocase
    • Duong F. Binding, activation and dissociation of the dimeric SecA ATPase at the dimeric SecYEG translocase. Embo J, 2003, 22: 4375-4384.
    • (2003) Embo J , vol.22 , pp. 4375-4384
    • Duong, F.1


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