Immunogold localisation of P-glycoprotein in supported lipid bilayers by transmission electron microscopy and atomic force microscopy

Histochemical Journal

Volumn 33, Issue 5, 2001, Pages 305-309

  Ruspantini, Irene ^a   Diociaiuti, Marco ^a   Ippoliti, Rodolfo ^b   Lendaro, Eugenio ^b   Cristina Gaudiano, Maria ^a   Cianfriglia, Maurizio ^a   Chistolini, Pietro ^a   Arancia, Giuseppe ^a   Molinari, Agnese ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON; EPITOPE; GLYCOPROTEIN P; LIPOSOME; MICA; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY MM4.17; MONOCLONAL ANTIBODY MRK 16; UNCLASSIFIED DRUG;

ARTICLE; ATOMIC FORCE MICROSCOPY; BIOSENSOR; CONTROLLED STUDY; HUMAN; HUMAN CELL; HYDROPHOBICITY; IMMUNOGOLD STAINING; LEUKEMIA CELL; LIPID BILAYER; LIPID MONOLAYER; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN CONFORMATION; PROTEIN LOCALIZATION; TRANSMISSION ELECTRON MICROSCOPY;

HUMANS; IMMUNOHISTOCHEMISTRY; LIPID BILAYERS; LIPOSOMES; MICROSCOPY, ATOMIC FORCE; MICROSCOPY, IMMUNOELECTRON; P-GLYCOPROTEIN; TUMOR CELLS, CULTURED;

MICA;

EID: 0034861414     PISSN: 00182214     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1017933225226     Document Type: Article

References (15)

1. P-glycoprotein epitope mapping. I. Identification of a linear human-specific epitope in the fourth loop of the P-glycoprotein extracellular domain by MM4.17 murine monoclonal antibody to human multi-drug-resistant cells
2. Efficient purification and reconstitution of P-glycoprotein for functional and structural studies
3. Cell-surface receptor and proteins on platelet membranes imaged by scanning force microscopy using immunogold contrast enhancement
4. Topology of P-glycoprotein as determined by epitope mapping of MRK-16 antibody
5. Multidrug resistance in human cancer
6. Structure of multidrug resistance p-glycoprotein
7. Modulation of human P-glycoprotein epitope expression by temperature and/or resistance-modulating agents
8. Symmetry and structure in P-glycoprotein and ABC transporters
9. A surface glycoprotein modulating drug permeability in Chinese hamster ovary mutants
10. The minimum functional unit of human p-glycoprotein appears to be a monomer
11. P-glycoprotein function involves conformational transitions detectable by differential immunoreactivity
12. P-glycoprotein expression in the golgi apparatus of multidrug-resistant cells
13. Planar lipid bilayers on solid supports from liposomes: Factors of importance for kinetics and stability
14. Reconstitution of membrane proteins into liposomes: Application to energy-transducing membrane proteins