The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure

EMBO Journal

Volumn 21, Issue 5, 2002, Pages 995-1003

  Bessonneau, Pascal ^a   Besson, Véronique ^a   Collinson, Ian ^a   Duong, Franck ^a  

^a INSTITUTE FOR INTEGRATIVE BIOLOGY OF THE CELL I2BC   (France)

Author keywords

Blue native gel electrophoresis; Membrane protein oligomerisation; Preprotein translocase; SecYEGA; Translocation channel

Indexed keywords

CARRIER PROTEIN; DETERGENT; DIMER; MONOMER; POLYMER; PROTEIN PRECURSOR; PROTEIN SECE; PROTEIN SECG; PROTEIN SECYEG; SECY PROTEIN; TETRAMER; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL MEMBRANE; COMPLEX FORMATION; CONCENTRATION RESPONSE; CROSS LINKING; DIMERIZATION; DISSOCIATION; ENZYME IMMOBILIZATION; ESCHERICHIA COLI; GEL ELECTROPHORESIS; MEMBRANE CHANNEL; MOLECULAR DYNAMICS; NONHUMAN; OLIGOMERIZATION; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN SECRETION; PROTEIN TRANSPORT;

ADENOSINE TRIPHOSPHATASES; BACTERIAL PROTEINS; DIMERIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MACROMOLECULAR SUBSTANCES; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MOTION; PROTEIN CONFORMATION; PROTEIN PRECURSORS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN TRANSPORT; STRUCTURE-ACTIVITY RELATIONSHIP;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0036500974     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/21.5.995     Document Type: Article

References (41)

1. Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex
2. SecY, SecE and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase
3. Projection structure and oligomeric properties of a bacterial core protein translocase
4. Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coli
5. Precusor protein translocation by the Escherichia coli translocase is directed by the protonmotive force
6. The structural basis of protein targeting and translocation in bacteria
7. Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme
8. The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling
9. Sec-dependent membrane protein biogenesis: SecYEG, preprotein hydrophobicity and translocation kinetics control the stop-transfer function
10. The PrlA and Prig phenotypes are caused by a loosened association among the translocase SecYEG subunits
11. Bacterial preprotein translocase: Mechanism and conformational dynamics of a processive enzyme
12. Seca promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion
13. Archaeal protein translocation crossing membranes in the third domain of life
14. Both an N-terminal 65-kDa domain and a C-terminal 30-kDa domain of SecA cycle into the m embrane at SecYEG during translocation
15. SecG is an auxiliary component of the protein export apparatus of Escherichia coli
16. BiP maintains the permeability barrier of the ER membrane by sealing the lumenal end of the translocon pore before and early in translocation
17. SecG plays a critical role in protein translocation in the absence of the proton motive force as well as at low temperature
18. Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation
19. The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. Coli plasma membrane
20. Evolutionary conservation of components of the protein translocation complex
21. The Sec61p complex mediates the integration of a membrane protein by allowing lipid partitioning of the transmembrane domain
22. SecA protein needs both acidic phospholipids and SecY/E protein for functional high-affinity binding to the Escherichia coli plasma membrane
23. Subunit dynamics in Escherichia coli preprotein translocase
24. The ATPase activity of Seca is regulated by acidic phospholipids, SecY, and the leader and mature domains of precursor proteins
25. In vivo cross-linking of the Seca and SecY subunits of the Escherichia coli preprotein translocase
26. SecYEG assembles into a tetramer to form the active protein translocation channel
27. The protein-conducting channel in the membrane of the endoplasmic reticulum is open laterally toward the lipid bilayer
28. The structure of ribosome-channel complexes engaged in protein translocation
29. The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic sec61p complex
30. Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane
31. Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation
32. Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes
33. Blue-native gels to isolate protein complexes from mitochodria
34. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form
35. + and ATP function at different steps of the catalytic cycle of preprotein translocase
36. YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase
37. A protein-conducting channel in the endoplasmic reticulum
38. Signal peptides open protein-conducting channels in E. Coli
39. Translocation of ProOmpA possessing an intramolecular disulfide bridge into membrane vesicles of Escherichia coil Effect of membrane energization
40. PrlA4 prevents the rejection of signal sequence defective preproteins by stabilizing the SecA-SecY interaction during the initiation of translocation
41. Evaluating the oligomeric state of SecYEG in preprotein translocase