메뉴 건너뛰기




Volumn 3, Issue 3-4, 2010, Pages 179-191

Gilthead sea bream (Sparus auratus) and European sea bass (Dicentrarchus labrax) expressed sequence tags: Characterization, tissue-specific expression and gene markers

(16)  Louro, Bruno a,j   Passos, Ana Lúcia S a   Souche, Erika L b,k   Tsigenopoulos, Costas c   Beck, Alfred d   Lagnel, Jacques c   Bonhomme, François e   Cancela, Leonor a   Cerdà, Joan f   Clark, Melody S g   Lubzens, Esther h   Magoulas, Antonis c   Planas, Josep V i   Volckaert, Filip A M b   Reinhardt, Richard d   Canario, Adelino V M a  


Author keywords

Annotation; Aquaculture; Expressed Sequence Tag; Microsatellite; Single nucleotide polymorphism; Teleost fish

Indexed keywords

ARCHOSARGUS RHOMBOIDALIS; DICENTRARCHUS LABRAX; GASTEROSTEUS ACULEATUS; SERRANIDAE; SPARUS AURATA; TELEOSTEI;

EID: 78649495378     PISSN: 18747787     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.margen.2010.09.005     Document Type: Article
Times cited : (27)

References (68)
  • 1
    • 34447299402 scopus 로고    scopus 로고
    • Annotated Expressed Sequence Tags (ESTs) from pre-smolt atlantic salmon (Salmo salar) in a searchable data resource
    • Adzhubei A.A., Vlasova A.V., Hagen-Larsen H., Ruden T.A., Laerdahl J.K., Hoyheim B. Annotated Expressed Sequence Tags (ESTs) from pre-smolt atlantic salmon (Salmo salar) in a searchable data resource. BMC Genomics 2007, 8:209.
    • (2007) BMC Genomics , vol.8 , pp. 209
    • Adzhubei, A.A.1    Vlasova, A.V.2    Hagen-Larsen, H.3    Ruden, T.A.4    Laerdahl, J.K.5    Hoyheim, B.6
  • 4
    • 0008203899 scopus 로고    scopus 로고
    • The stress response of gilthead sea bream (Sparus aurata L) to air exposure and confinement
    • Arends R., Mancera J., Munoz J., Wendelaar Bonga S., Flick G. The stress response of gilthead sea bream (Sparus aurata L) to air exposure and confinement. J. Endocrinol. 1999, 143:23-31.
    • (1999) J. Endocrinol. , vol.143 , pp. 23-31
    • Arends, R.1    Mancera, J.2    Munoz, J.3    Wendelaar Bonga, S.4    Flick, G.5
  • 6
    • 78649510357 scopus 로고    scopus 로고
    • Genetic variation analysis for biomedical researchers: A primer, in,
    • Barnes, M.R. Genetic variation analysis for biomedical researchers: A primer, in, 2009, pp. 1-20.
    • (2009) , pp. 1-20
    • Barnes, M.R.1
  • 7
    • 0037986743 scopus 로고    scopus 로고
    • Mining for single nucleotide polymorphisms and insertions/deletions in maize expressed sequence tag data
    • Batley J., Barker G., O'Sullivan H., Edwards K.J., Edwards D. Mining for single nucleotide polymorphisms and insertions/deletions in maize expressed sequence tag data. Plant Physiol. 2003, 132:84-91.
    • (2003) Plant Physiol. , vol.132 , pp. 84-91
    • Batley, J.1    Barker, G.2    O'Sullivan, H.3    Edwards, K.J.4    Edwards, D.5
  • 8
    • 0036295210 scopus 로고    scopus 로고
    • Molecular analysis of expansion, differentiation, and growth factor treatment of human chondrocytes identifies differentiation markers and growth-related genes
    • Benz K., Breit S., Lukoschek M., Mau H., Richter W. Molecular analysis of expansion, differentiation, and growth factor treatment of human chondrocytes identifies differentiation markers and growth-related genes. Biochem. Biophys. Res. Commun. 2002, 293:284-292.
    • (2002) Biochem. Biophys. Res. Commun. , vol.293 , pp. 284-292
    • Benz, K.1    Breit, S.2    Lukoschek, M.3    Mau, H.4    Richter, W.5
  • 9
    • 0345051044 scopus 로고    scopus 로고
    • Normalization and subtraction: two approaches to facilitate gene discovery
    • Bonaldo M.F., Lennon G., Soares M.B. Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res. 1996, 6:791-806.
    • (1996) Genome Res. , vol.6 , pp. 791-806
    • Bonaldo, M.F.1    Lennon, G.2    Soares, M.B.3
  • 10
    • 0035047940 scopus 로고    scopus 로고
    • G protein-coupled prostanoid receptors and the kidney
    • Breyer M.D., Breyer R.M. G protein-coupled prostanoid receptors and the kidney. Annu. Rev. Physiol. 2001, 63:579-605.
    • (2001) Annu. Rev. Physiol. , vol.63 , pp. 579-605
    • Breyer, M.D.1    Breyer, R.M.2
  • 11
    • 77952275950 scopus 로고    scopus 로고
    • Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.)
    • Calduch-Giner J., Davey G., Saera-Vila A., Houeix B., Talbot A., Prunet P., Cairns M., Perez-Sanchez J. Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.). BMC Genomics 2010, 11:193.
    • (2010) BMC Genomics , vol.11 , pp. 193
    • Calduch-Giner, J.1    Davey, G.2    Saera-Vila, A.3    Houeix, B.4    Talbot, A.5    Prunet, P.6    Cairns, M.7    Perez-Sanchez, J.8
  • 14
    • 3042615882 scopus 로고    scopus 로고
    • Using the miraEST assembler for reliable and automated mRNA transcript assembly and SNP detection in sequenced ESTs
    • Chevreux B., Pfisterer T., Drescher B., Driesel A.J., Muller W.E., Wetter T., Suhai S. Using the miraEST assembler for reliable and automated mRNA transcript assembly and SNP detection in sequenced ESTs. Genome Res. 2004, 14:1147-1159.
    • (2004) Genome Res. , vol.14 , pp. 1147-1159
    • Chevreux, B.1    Pfisterer, T.2    Drescher, B.3    Driesel, A.J.4    Muller, W.E.5    Wetter, T.6    Suhai, S.7
  • 15
    • 33746932130 scopus 로고    scopus 로고
    • EST-based identification of genes expressed in the liver of adult seabass (Dicentrarchus labrax, L.)
    • Chini V., Rimoldi S., Terova G., Saroglia M., Rossi F., Bernardini G., Gornati R. EST-based identification of genes expressed in the liver of adult seabass (Dicentrarchus labrax, L.). Gene 2006, 376:102-106.
    • (2006) Gene , vol.376 , pp. 102-106
    • Chini, V.1    Rimoldi, S.2    Terova, G.3    Saroglia, M.4    Rossi, F.5    Bernardini, G.6    Gornati, R.7
  • 16
    • 0023277545 scopus 로고
    • Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
    • Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 1987, 162:156-159.
    • (1987) Anal. Biochem. , vol.162 , pp. 156-159
    • Chomczynski, P.1    Sacchi, N.2
  • 17
    • 0036137277 scopus 로고    scopus 로고
    • DNA sequence quality trimming and vector removal
    • Chou H.H., Holmes M.H. DNA sequence quality trimming and vector removal. Bioinformatics 2001, 17:1093-1104.
    • (2001) Bioinformatics , vol.17 , pp. 1093-1104
    • Chou, H.H.1    Holmes, M.H.2
  • 19
    • 33646349713 scopus 로고    scopus 로고
    • Expressed sequence tags from normalized cDNA libraries prepared from gill and hypodermal tissues of the blue crab, Callinectes sapidus
    • Coblentz F.E., Towle D.W., Shafer T.H. Expressed sequence tags from normalized cDNA libraries prepared from gill and hypodermal tissues of the blue crab, Callinectes sapidus. Comp. Biochem. Physiol. Part D Genomics Proteomics 2006, 1:200-208.
    • (2006) Comp. Biochem. Physiol. Part D Genomics Proteomics , vol.1 , pp. 200-208
    • Coblentz, F.E.1    Towle, D.W.2    Shafer, T.H.3
  • 20
    • 0033195096 scopus 로고    scopus 로고
    • Winter flounder expressed sequence tags: establishment of an EST database and identification of novel fish genes
    • Douglas S., Gallant J., Bullerwell C., Wolff C., Munholland J., Reith M. Winter flounder expressed sequence tags: establishment of an EST database and identification of novel fish genes. Mar. Biotechnol. 1999, 1:458-464.
    • (1999) Mar. Biotechnol. , vol.1 , pp. 458-464
    • Douglas, S.1    Gallant, J.2    Bullerwell, C.3    Wolff, C.4    Munholland, J.5    Reith, M.6
  • 21
    • 34447527051 scopus 로고    scopus 로고
    • Comprehensive EST analysis of Atlantic halibut (Hippoglossus hippoglossus), a commercially relevant aquaculture species
    • Douglas S.E., Knickle L.C., Kimball J., Reith M.E. Comprehensive EST analysis of Atlantic halibut (Hippoglossus hippoglossus), a commercially relevant aquaculture species. BMC Genomics 2007, 8:144.
    • (2007) BMC Genomics , vol.8 , pp. 144
    • Douglas, S.E.1    Knickle, L.C.2    Kimball, J.3    Reith, M.E.4
  • 22
    • 34247254351 scopus 로고    scopus 로고
    • Novel peptide identification from tandem mass spectra using ESTs and sequence database compression
    • Edwards N.J. Novel peptide identification from tandem mass spectra using ESTs and sequence database compression. Mol. Syst. Biol. 2007, 3:102.
    • (2007) Mol. Syst. Biol. , vol.3 , pp. 102
    • Edwards, N.J.1
  • 23
    • 0031978181 scopus 로고    scopus 로고
    • Base-calling of automated sequencer traces using phred. II. Error probabilities
    • Ewing B., Green P. Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res. 1998, 8:186-194.
    • (1998) Genome Res. , vol.8 , pp. 186-194
    • Ewing, B.1    Green, P.2
  • 24
    • 0031955518 scopus 로고    scopus 로고
    • Base-calling of automated sequencer traces using phred. I. Accuracy assessment
    • Ewing B., Hillier L., Wendl M.C., Green P. Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res. 1998, 8:175-185.
    • (1998) Genome Res. , vol.8 , pp. 175-185
    • Ewing, B.1    Hillier, L.2    Wendl, M.C.3    Green, P.4
  • 27
    • 0032721621 scopus 로고    scopus 로고
    • Identification of candidate coding region single nucleotide polymorphisms in 165 human genes using assembled expressed sequence tags
    • Garg K., Green P., Nickerson D.A. Identification of candidate coding region single nucleotide polymorphisms in 165 human genes using assembled expressed sequence tags. Genome Res. 1999, 9:1087-1092.
    • (1999) Genome Res. , vol.9 , pp. 1087-1092
    • Garg, K.1    Green, P.2    Nickerson, D.A.3
  • 28
    • 0032622083 scopus 로고    scopus 로고
    • Zebrafish expressed sequence tags and their applications
    • Gong Z. Zebrafish expressed sequence tags and their applications. Methods Cell Biol. 1999, 60:213-233.
    • (1999) Methods Cell Biol. , vol.60 , pp. 213-233
    • Gong, Z.1
  • 29
    • 0030817790 scopus 로고    scopus 로고
    • Rapid identification and isolation of zebrafish cDNA clones
    • Gong Z., Yan T., Liao J., Lee S., He J., Hew C. Rapid identification and isolation of zebrafish cDNA clones. Gene 1997, 201:87-98.
    • (1997) Gene , vol.201 , pp. 87-98
    • Gong, Z.1    Yan, T.2    Liao, J.3    Lee, S.4    He, J.5    Hew, C.6
  • 31
    • 33748697895 scopus 로고    scopus 로고
    • Generation of a large scale repertoire of Expressed Sequence Tags (ESTs) from normalised rainbow trout cDNA libraries
    • Govoroun M., Le Gac F., Guiguen Y. Generation of a large scale repertoire of Expressed Sequence Tags (ESTs) from normalised rainbow trout cDNA libraries. BMC Genomics 2006, 7:196.
    • (2006) BMC Genomics , vol.7 , pp. 196
    • Govoroun, M.1    Le Gac, F.2    Guiguen, Y.3
  • 32
    • 29144462664 scopus 로고    scopus 로고
    • An EST-based approach for identifying genes expressed in the intestine and gills of pre-smolt atlantic salmon (Salmo salar)
    • Hagen-Larsen H., Laerdahl J.K., Panitz F., Adzhubei A., Høyheim B. An EST-based approach for identifying genes expressed in the intestine and gills of pre-smolt atlantic salmon (Salmo salar). BMC Genomics 2005, 6:171.
    • (2005) BMC Genomics , vol.6 , pp. 171
    • Hagen-Larsen, H.1    Laerdahl, J.K.2    Panitz, F.3    Adzhubei, A.4    Høyheim, B.5
  • 33
    • 48449095902 scopus 로고    scopus 로고
    • Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon
    • Haugarvoll E., Bjerkas I., Nowak B.F., Hordvik I., Koppang E.O. Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon. J. Anat. 2008, 213:202-209.
    • (2008) J. Anat. , vol.213 , pp. 202-209
    • Haugarvoll, E.1    Bjerkas, I.2    Nowak, B.F.3    Hordvik, I.4    Koppang, E.O.5
  • 34
    • 0031449827 scopus 로고    scopus 로고
    • Expressed sequence tags of medaka (Oryzias latipes) liver mRNA
    • Hirono I., Aoki T. Expressed sequence tags of medaka (Oryzias latipes) liver mRNA. Mol. Mar. Biol. Biotechnol. 1997, 6:345-350.
    • (1997) Mol. Mar. Biol. Biotechnol. , vol.6 , pp. 345-350
    • Hirono, I.1    Aoki, T.2
  • 35
    • 33244486093 scopus 로고    scopus 로고
    • An in silico mining for simple sequence repeats from expressed sequence tags of zebrafish, medaka, Fundulus, and Xiphophorus
    • Ju Z., Wells M.C., Martinez A., Hazlewood L., Walter R.B. An in silico mining for simple sequence repeats from expressed sequence tags of zebrafish, medaka, Fundulus, and Xiphophorus. In Silico Biol. 2005, 5:439-463.
    • (2005) In Silico Biol. , vol.5 , pp. 439-463
    • Ju, Z.1    Wells, M.C.2    Martinez, A.3    Hazlewood, L.4    Walter, R.B.5
  • 36
    • 0036226603 scopus 로고    scopus 로고
    • BLAT- the BLAST-like alignment tool
    • Kent W.J. BLAT- the BLAST-like alignment tool. Genome Res. 2002, 12:656-664.
    • (2002) Genome Res. , vol.12 , pp. 656-664
    • Kent, W.J.1
  • 38
    • 77649287723 scopus 로고    scopus 로고
    • The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing
    • Kuhl H., Beck A., Wozniak G., Canario A.V.M., Volckaert F.A.M., Reinhardt R. The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing. BMC Genomics 2010, 11:68.
    • (2010) BMC Genomics , vol.11 , pp. 68
    • Kuhl, H.1    Beck, A.2    Wozniak, G.3    Canario, A.V.M.4    Volckaert, F.A.M.5    Reinhardt, R.6
  • 42
    • 12744261322 scopus 로고    scopus 로고
    • Maintenance of genetic differentiation across a transition zone in the sea: discordance between nuclear and cytoplasmic markers
    • Lemaire C., Versini J.J., Bonhomme F. Maintenance of genetic differentiation across a transition zone in the sea: discordance between nuclear and cytoplasmic markers. J. Evolution Biol. 2005, 18:70-80.
    • (2005) J. Evolution Biol. , vol.18 , pp. 70-80
    • Lemaire, C.1    Versini, J.J.2    Bonhomme, F.3
  • 45
    • 0030947025 scopus 로고    scopus 로고
    • Spontaneous calcification of arteries and cartilage in mice lacking matrix GLA protein
    • Luo G., Ducy P., McKee M.D., Pinero G.J., Loyer E., Behringer R.R., Karsenty G. Spontaneous calcification of arteries and cartilage in mice lacking matrix GLA protein. Nature 1997, 386:78-81.
    • (1997) Nature , vol.386 , pp. 78-81
    • Luo, G.1    Ducy, P.2    McKee, M.D.3    Pinero, G.J.4    Loyer, E.5    Behringer, R.R.6    Karsenty, G.7
  • 47
    • 0036557834 scopus 로고    scopus 로고
    • Physiological changes in the spawning gilthead seabream, Sparus aurata, succeeding the removal of males
    • Meiri I., Gothilf Y., Zohar Y., Elizur A. Physiological changes in the spawning gilthead seabream, Sparus aurata, succeeding the removal of males. J. Exp. Zool. 2002, 292:555-564.
    • (2002) J. Exp. Zool. , vol.292 , pp. 555-564
    • Meiri, I.1    Gothilf, Y.2    Zohar, Y.3    Elizur, A.4
  • 48
    • 53649088131 scopus 로고    scopus 로고
    • Applications of next-generation sequencing technologies in functional genomics
    • Morozova O., Marra M.A. Applications of next-generation sequencing technologies in functional genomics. Genomics 2008, 92:255-264.
    • (2008) Genomics , vol.92 , pp. 255-264
    • Morozova, O.1    Marra, M.A.2
  • 49
    • 25444475269 scopus 로고    scopus 로고
    • FunnyBase: a systems level functional annotation of Fundulus ESTs for the analysis of gene expression
    • DL C.
    • Paschall J., Oleksiak M., VanWye J., Roach J., Whitehead J., Wyckoff G., Kolell K., DL C. FunnyBase: a systems level functional annotation of Fundulus ESTs for the analysis of gene expression. BMC Genomics 2004, 5:96.
    • (2004) BMC Genomics , vol.5 , pp. 96
    • Paschall, J.1    Oleksiak, M.2    VanWye, J.3    Roach, J.4    Whitehead, J.5    Wyckoff, G.6    Kolell, K.7
  • 50
    • 34447505998 scopus 로고    scopus 로고
    • Migrations, fishery interactions, and management units of sea bass (Dicentrarchus labrax) in Northwest Europe
    • Pawson M.G., Pickett G.D., Leballeur J., Brown M., Fritsch M. Migrations, fishery interactions, and management units of sea bass (Dicentrarchus labrax) in Northwest Europe. ICES J. Mar. Sci. 2007, 64:332-345.
    • (2007) ICES J. Mar. Sci. , vol.64 , pp. 332-345
    • Pawson, M.G.1    Pickett, G.D.2    Leballeur, J.3    Brown, M.4    Fritsch, M.5
  • 52
    • 18144400545 scopus 로고    scopus 로고
    • Genetic, endocrine, and environmental components of sex determination and differentiation in the European sea bass (Dicentrarchus labrax L.)
    • Piferrer F., Blazquez M., Navarro L., Gonzalez A. Genetic, endocrine, and environmental components of sex determination and differentiation in the European sea bass (Dicentrarchus labrax L.). Gen. Comp. Endocrinol. 2005, 142:102-110.
    • (2005) Gen. Comp. Endocrinol. , vol.142 , pp. 102-110
    • Piferrer, F.1    Blazquez, M.2    Navarro, L.3    Gonzalez, A.4
  • 56
    • 4344695759 scopus 로고    scopus 로고
    • Bioinformatic mining of type I microsatellites from expressed sequence tags of channel catfish (Ictalurus punctatus)
    • Serapion J., Kucuktas H., Feng J., Liu Z. Bioinformatic mining of type I microsatellites from expressed sequence tags of channel catfish (Ictalurus punctatus). Mar. Biotechnol. 2004, 6:364-377.
    • (2004) Mar. Biotechnol. , vol.6 , pp. 364-377
    • Serapion, J.1    Kucuktas, H.2    Feng, J.3    Liu, Z.4
  • 57
    • 78649500873 scopus 로고    scopus 로고
    • RepeatMasker Open-3.0
    • Smit, A.F.A., Hubley, R., Green, P. RepeatMasker Open-3.0, 1996-2010 http://www.repeatmasker.org.
    • Smit, A.F.A.1    Hubley, R.2    Green, P.3
  • 58
    • 0023164446 scopus 로고
    • Evidence that PGE2 stimulates intestinal epithelial cell adenylate cyclase by a receptor-mediated mechanism
    • Smith G., Warhurst G., Lees M., Turnberg L. Evidence that PGE2 stimulates intestinal epithelial cell adenylate cyclase by a receptor-mediated mechanism. Dig. Dis. Sci. 1987, 32:71-75.
    • (1987) Dig. Dis. Sci. , vol.32 , pp. 71-75
    • Smith, G.1    Warhurst, G.2    Lees, M.3    Turnberg, L.4
  • 60
    • 0031574072 scopus 로고    scopus 로고
    • The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools
    • Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997, 25:4876-4882.
    • (1997) Nucleic Acids Res. , vol.25 , pp. 4876-4882
    • Thompson, J.D.1    Gibson, T.J.2    Plewniak, F.3    Jeanmougin, F.4    Higgins, D.G.5
  • 61
    • 0033858199 scopus 로고    scopus 로고
    • Microsatellites in different eukaryotic genomes: survey and analysis
    • Tóth G., Gáspári Z., Jurka J. Microsatellites in different eukaryotic genomes: survey and analysis. Genome Res. 2000, 10:967-981.
    • (2000) Genome Res. , vol.10 , pp. 967-981
    • Tóth, G.1    Gáspári, Z.2    Jurka, J.3
  • 62
    • 78649506617 scopus 로고    scopus 로고
    • In silico mining and characterization of simple sequence repeats from gilthead sea bream (Sparus aurata) expressed sequence tags (EST-SSRs); PCR amplification, polymorphism evaluation and multiplexing and cross-species assays, Marine Genomics, (submitted).
    • Vogiatzi, E., Lagnel, J., Pakaki, V., Louro, B., Canario, A.V.M., Reinhardt, R., Kotoulas, G., Magoulas, A., Tsigenopoulos, C.S. In silico mining and characterization of simple sequence repeats from gilthead sea bream (Sparus aurata) expressed sequence tags (EST-SSRs); PCR amplification, polymorphism evaluation and multiplexing and cross-species assays, Marine Genomics, (submitted).
    • Vogiatzi, E.1    Lagnel, J.2    Pakaki, V.3    Louro, B.4    Canario, A.V.M.5    Reinhardt, R.6    Kotoulas, G.7    Magoulas, A.8    Tsigenopoulos, C.S.9
  • 64
    • 0035189310 scopus 로고    scopus 로고
    • Spidey: a tool for mRNA-to-genomic alignments
    • Wheelan S.J., Church D.M., Ostell J.M. Spidey: a tool for mRNA-to-genomic alignments. Genome Res. 2001, 11:1952-1957.
    • (2001) Genome Res. , vol.11 , pp. 1952-1957
    • Wheelan, S.J.1    Church, D.M.2    Ostell, J.M.3
  • 66
    • 67650721347 scopus 로고    scopus 로고
    • CateGOrizer: A web-based program to batch gene ontology classification categories, in
    • Zhi-Liang H., Bao J., Reecy J.M. CateGOrizer: A web-based program to batch gene ontology classification categories, in. Online J. Bioinformatics 2008, 5.
    • (2008) Online J. Bioinformatics , pp. 5
    • Zhi-Liang, H.1    Bao, J.2    Reecy, J.M.3
  • 67
    • 0035046755 scopus 로고    scopus 로고
    • Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction
    • Zhu Y.Y., Machleder E.M., Chenchik A., Li R., Siebert P.D. Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction. Biotechniques 2001, 30:892-897.
    • (2001) Biotechniques , vol.30 , pp. 892-897
    • Zhu, Y.Y.1    Machleder, E.M.2    Chenchik, A.3    Li, R.4    Siebert, P.D.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.