Molecular analysis of expansion, differentiation, and growth factor treatment of human chondrocytes identifies differentiation markers and growth-related genes

Biochemical and Biophysical Research Communications

Volumn 293, Issue 1, 2002, Pages 284-292

  Benz, Karin ^a   Breit, Stephen ^a   Lukoschek, Martin ^a   Mau, Hans ^a   Richter, Wiltrud ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

3D culture; Bone morphogenic proteins; Cartilage; Collagen; Proteoglycan; Tissue engineering

Indexed keywords

ALGINIC ACID; BASIC FIBROBLAST GROWTH FACTOR; BONE MORPHOGENETIC PROTEIN 2; BONE MORPHOGENETIC PROTEIN 4; BONE MORPHOGENETIC PROTEIN 6; COMPLEMENTARY DNA; EPIDERMAL GROWTH FACTOR; FIBROMODULIN; GROWTH DIFFERENTIATION FACTOR 5; GROWTH FACTOR; OSTEOGENIC PROTEIN 1; PLATELET DERIVED GROWTH FACTOR BB; SOMATOMEDIN C; TRANSFORMING GROWTH FACTOR BETA1;

ADOLESCENT; ADULT; AGED; ARTICLE; CARTILAGE CELL; CELL DIFFERENTIATION; CELL GROWTH; CLINICAL ARTICLE; CONTROLLED STUDY; DNA MICROARRAY; DOWN REGULATION; EXTRACELLULAR MATRIX; GENE EXPRESSION; GENE IDENTIFICATION; HUMAN; HUMAN TISSUE; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL;

BIOLOGICAL MARKERS; BONE MORPHOGENETIC PROTEINS; CELL DIFFERENTIATION; CELL DIVISION; CELLS, CULTURED; CHONDROCYTES; GROWTH SUBSTANCES; HUMANS; INSULIN-LIKE GROWTH FACTOR I; KINETICS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PLATELET-DERIVED GROWTH FACTOR; PROTEINS; TRANSFORMING GROWTH FACTOR BETA;

EID: 0036295210     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(02)00223-1     Document Type: Article

References (30)

1. Expression of cartilage-specific molecules is retained on long-term culture of human articular chondrocytes
2. Phenotypic stability of articular chondrocytes in vitro: The effects of culture models bone morphogenetic protein 2, and serum supplementation
3. The repair of experimentally produced defects in rabbit articular cartilage by autologous chondrocyte transplantation
4. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation
5. Two- to 9-year outcome after autologous chondrocyte transplantation of the knee
6. Expression of the human chondrocyte phenotype in vitro
7. Reexpression of cartilage-specific genes by dedifferentiated human articular chondrocytes cultured in alginate beads
8. Phenotypic modulation in sub-populations of human articular chondrocytes in vitro
9. Molecular markers predictive of the capacity of expanded human articular chondrocytes to form stable cartilage in vivo
10. Recombinant human bone morphogenetic protein-2 maintains the articular chondrocyte phenotype in long-term culture
11. Recombinant bone morphogenetic protein-4 transforming growth factor-beta 1 and activin A enhance the cartilage phenotype of articular chondrocytes in vitro
12. Effects of basic fibroblast growth factor transforming growth factor-beta 1, insulin-like growth factor-1 and insulin on human osteoarthritic articular cartilage explants
13. BMP-6 is an autocrine stimulator of chondrocyte differentiation
14. Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads
15. Quantification of 35S-labeled proteoglycans complexed to alcian blue by rapid filtration in multiwell plates
16. Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture
17. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels
18. Regulation of cartilage oligomeric matrix protein synthesis in human synovial cells and articular chondrocytes
19. Differentiation of mesenchymal limb bud cells to chondrocytes in alginate beads
20. Chondrocyte expressed protein-68 (CEP-68) a novel human marker gene for cultured chondrocytes
21. Specific growth factors during the expansion and redifferentiation of adult human articular chondrocytes enhance chondrogenesis and cartilaginous tissue formation in vitro
22. Effect of transforming growth factor beta on cell proliferation and glycosaminoglycan synthesis by rabbit growth-plate chondrocytes in culture
23. Growth factor actions on articular cartilage
24. Growth factor responsiveness of human articular chondrocytes: Distinct profiles in primary chondrocytes subcultured chondrocytes and fibroblasts
25. Bone morphogenetic proteins (BMP-2 and BMP-3) promote growth and expression of the differentiated phenotype of rabbit chondrocytes and osteoblastic MC3T3-E1 cells in vitro
26. Natural bovine osteogenin and recombinant human bone morphogenetic protein-2B are equipotent in the maintenance of proteoglycans in bovine articular cartilage explant cultures
27. Bone morphogenetic protein signaling is required for maintenance of differentiated phenotype control of proliferation and hypertrophy in chondrocytes
28. Growth factor responsiveness of human articular chondrocytes in aging and development
29. Cartilage tissue engineering: Current limitations and solutions
30. The evolving concept of a stem cell: Entity or function?