A green fluorescent protein solubility screen in E. coli reveals domain boundaries of the GTP-binding domain in the P element transposase

Protein Science

Volumn 19, Issue 11, 2010, Pages 2210-2218

  Sabogal, Alex ^a   Rio, Donald C ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Domain; Green fluorescent protein; Guanosine triphosphate; P element; Screen; Solubility; THAP domain

Indexed keywords

AMINO ACID; GREEN FLUORESCENT PROTEIN; GUANOSINE TRIPHOSPHATE; LEUCINE ZIPPER PROTEIN; TRANSPOSASE;

AMINO ACID SEQUENCE; ARTICLE; CLONE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DIMERIZATION; DNA STRAND BREAKAGE; DROSOPHILA MELANOGASTER; ESCHERICHIA COLI; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION; SOLUBILITY; TRANSPOSON;

ANIMALS; BINDING SITES; CELL LINE; CLONING, MOLECULAR; DNA FOOTPRINTING; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE LIBRARY; GREEN FLUORESCENT PROTEINS; GUANOSINE TRIPHOSPHATE; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SOLUBILITY; TRANSPOSASES;

DROSOPHILA MELANOGASTER;

EID: 78349285120     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.499     Document Type: Article

References (31)

1. Lee CC, Beall EL, Rio DC (1998) DNA binding by the KP repressor protein inhibits P-element transposase activity in vitro. EMBO J 17:4166-4174.
2. Lee CC, Mul YM, Rio DC (1996) The Drosophila P-element KP repressor protein dimerizes and interacts with multiple sites on P-element DNA. Mol Cell Biol 16:5616-5622.
3. Kaufman PD, Rio DC (1992) P element transposition in vitro proceeds by a cut-and-paste mechanism and uses GTP as a cofactor. Cell 69:27-39.
4. Mul YM, Rio DC (1997) Reprogramming the purine nucleotide cofactor requirement of Drosophila P element transposase in vivo. EMBO J 16:4441-4447.
5. Kaufman PD, Doll RF, Rio DC (1989) Drosophila P element transposase recognizes internal P element DNA sequences. Cell 59:359-371.
6. Davies DR, Goryshin IY, Reznikoff WS, Rayment I (2000) Three-dimensional structure of the Tn5 synaptic complex transposition intermediate. Science 289:77-85.
7. Richardson JM, Zhang L, Marcos S, Finnegan DJ, Harding MM, Taylor P, Walkinshaw MD (2004) Expression, purification and preliminary crystallographic studies of a single-point mutant of Mos1 mariner transposase. Acta Crystallogr D Biol Crystallogr 60:962-964.
8. Wang JY, Ling H, Yang W, Craigie R (2001) Structure of a two-domain fragment of HIV-1 integrase: implications for domain organization in the intact protein. EMBO J 20:7333-7343.
9. Rice P, Mizuuchi K (1995) Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration. Cell 82:209-220.
10. Kim DR, Dai Y, Mundy CL, Yang W, Oettinger MA (1999) Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase. Genes Dev 13:3070-3080.
11. Barton GJ (1994) Scop: structural classification of proteins. Trends Biochem Sci 19:554-555.
12. Nowotny M, Gaidamakov SA, Crouch RJ, Yang W (2005) Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis. Cell 121:1005-1016.
13. Nowotny M (2009) Retroviral integrase superfamily: the structural perspective. EMBO Rep 10:144-151.
14. Schweidenback CT, Baker TA (2008) Dissecting the roles of MuB in Mu transposition: ATP regulation of DNA binding is not essential for target delivery. Proc Natl Acad Sci USA 105:12101-12107.
15. Tang M, Cecconi C, Bustamante C, Rio DC (2007) Analysis of P element transposase protein-DNA interactions during the early stages of transposition. J Biol Chem 282:29002-29012.
16. Tang M, Cecconi C, Kim H, Bustamante C, Rio DC (2005) Guanosine triphosphate acts as a cofactor to promote assembly of initial P-element transposase-DNA synaptic complexes. Genes Dev 19:1422-1425.
17. Georgiou G, Valax P (1996) Expression of correctly folded proteins in Escherichia coli. Curr Opin Biotechnol 7:190-197.
18. Baneyx F, Palumbo JL (2003) Improving heterologous protein folding via molecular chaperone and foldase coexpression. Methods Mol Biol 205:171-197.
19. Kapust RB, Waugh DS (1999) Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci 8:1668-1674.
20. Liu JW, Boucher Y, Stokes HW, Ollis DL (2006) Improving protein solubility: the use of the Escherichia coli dihydrofolate reductase gene as a fusion reporter. Protein Expr Purif 47:258-263.
21. Maxwell KL, Mittermaier AK, Forman-Kay JD, Davidson AR (1999) A simple in vivo assay for increased protein solubility. Protein Sci 8:1908-1911.
22. Cabantous S, Rogers Y, Terwilliger TC, Waldo GS (2008) New molecular reporters for rapid protein folding assays. PLoS ONE 3:e2387.
23. Kawasaki M, Inagaki F (2001) Random PCR-based screening for soluble domains using green fluorescent protein. Biochem Biophys Res Commun 280:842-844.
24. Jacobs SA, Podell ER, Wuttke DS, Cech TR (2005) Soluble domains of telomerase reverse transcriptase identified by high-throughput screening. Protein Sci 14:2051-2058.
25. Waldo GS, Standish BM, Berendzen J, Terwilliger TC (1999) Rapid protein-folding assay using green fluorescent protein. Nat Biotechnol 17:691-695.
26. Hammon J, Palanivelu DV, Chen J, Patel C, Minor DL, Jr (2009) A green fluorescent protein screen for identification of well-expressed membrane proteins from a cohort of extremophilic organisms. Protein Sci 18:121-133.
27. Sabogal A, Lyubimov AY, Corn JE, Berger JM, Rio DC (2010) THAP proteins target specific DNA sites through bipartite recognition of adjacent major and minor grooves. Nat Struct Mol Biol 17:117-123.
28. Roussigne M, Kossida S, Lavigne AC, Clouaire T, Ecochard V, Glories A, Amalric F, Girard JP (2003) The THAP domain: a novel protein motif with similarity to the DNA-binding domain of P element transposase. Trends Biochem Sci 28:66-69.
29. Bessiere D, Lacroix C, Campagne S, Ecochard V, Guillet V, Mourey L, Lopez F, Czaplicki J, Demange P, Milon A, Girard JP, Gervais V (2008) Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways. J Biol Chem 283:4352-4363.
30. Liew CK, Crossley M, Mackay JP, Nicholas HR (2007) Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP). J Mol Biol 366:382-390.
31. Landschulz WH, Johnson PF, McKnight SL (1988) The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science 240:1759-1764.