Improving protein solubility: The use of the Escherichia coli dihydrofolate reductase gene as a fusion reporter

Protein Expression and Purification

Volumn 47, Issue 1, 2006, Pages 258-263

  Liu, Jian Wei ^a   Boucher, Yan ^b   Stokes, H W ^b   Ollis, David L ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^b MACQUARIE UNIVERSITY   (Australia)

Author keywords

Dihydrofolate reductase; Directed evolution; Fusion protein; Gene cassette; Protein solubility

Indexed keywords

ACYLTRANSFERASE; DIHYDROFOLATE REDUCTASE; HYBRID PROTEIN;

ARTICLE; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; REPORTER GENE; SOLUBILITY;

ACETYLTRANSFERASES; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENES, REPORTER; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TETRAHYDROFOLATE DEHYDROGENASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; VIBRIO;

EID: 33646113145     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.11.019     Document Type: Article

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