Soluble domains of telomerase reverse transcriptase identified by high-throughput screening

Protein Science

Volumn 14, Issue 8, 2005, Pages 2051-2058

  Jacobs, Steven A ^a   Podell, Elaine R ^a   Wuttke, Deborah S ^a   Cech, Thomas R ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

GFP; High throughput; Telomerase; TERT

Indexed keywords

RECOMBINANT PROTEIN; TELOMERASE REVERSE TRANSCRIPTASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOPHYSICS; CONTROLLED STUDY; ESCHERICHIA COLI; MEDICAL INFORMATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; TETRAHYMENA THERMOPHILA;

AMINO ACID SEQUENCE; ANIMALS; DNA-BINDING PROTEINS; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; LUMINESCENT AGENTS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TELOMERASE; TETRAHYMENA THERMOPHILA;

ESCHERICHIA COLI; EUKARYOTA; TETRAHYMENA THERMOPHILA;

EID: 23644461031     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.051532105     Document Type: Article

References (26)

1. Armbruster, B.N., Banik, S.S., Guo, C., Smith, A.C., and Counter, C.M. 2001. N-terminal domains of the human telomerase catalytic subunit required for enzyme activity in vivo. Mol. Cell. Biol. 21: 7775-7786.
2. Bachand, F. and Autexier, C. 1999. Functional reconstitution of human telomerase expressed in Saccharomyces cerevisiae. J. Biol. Chem. 274: 38027-38031.
3. Beattie, T.L., Zhou, W., Robinson, M.O., and Harrington, L. 2001. Functional multimerization of the human telomerase reverse transcriptase. Mol. Cell. Biol. 21: 6151-6160.
4. Bryan, T.M., Goodrich, K.J., and Cech, T.R. 2000a. A mutant of Tetrahymena telomerase reverse transcriptase with increased processivity. J. Biol. Chem. 275: 24199-24207.
5. _. 2000b. Telomerase RNA bound by protein motifs specific to telomerase reverse transcriptase. Mol. Cell. 6: 493-499.
6. Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T.J., Higgins, D.G., and Thompson, J.D. 2003. Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res. 31: 3497-3500.
7. Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., and Bax, A. 1995. NMRPipe: A multidimensional spectral processing system based on UNIX pipes. J. Biomol. NMR 6: 277-293.
8. Friedman, K.L. and Cech, T.R. 1999. Essential functions of amino-terminal domains in the yeast telomerase catalytic subunit revealed by selection for viable mutants. Genes & Dev. 13: 2863-2874.
9. Friedman, K.L., Heit, J.J., Long, D.M., and Cech, T.R. 2003. N-terminal domain of yeast telomerase reverse transcriptase: Recruitment of Est3p to the telomerase complex. Mol. Biol. Cell 14: 1-13.
10. Greider, C.W. and Blackburn, E.H. 1989. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature 337: 331-337.
11. Grothues, D., Cantor, C.R., and Smith, C.L. 1993. PCR amplification of megabase DNA with tagged random primers (T-PCR). Nucleic Acids Res. 21: 1321-1322.
12. Johnson, B.A. and Blevins, R.A. 1994. NMRview: A computer program for the visualization and analysis of NMR data. J. Biomol. NMR 4: 603-614.
13. Kawasaki, M. and Inagaki, F. 2001. Random PCR-based screening for soluble domains using green fluorescent protein. Biochem. Biophys. Res. Commun. 280: 842-844.
14. Kelleher, C., Teixeira, M.T., Forstemann, K., and Lingner, J. 2002. Telomerase: Biochemical considerations for enzyme and substrate. Trends Biochem. Sci. 27: 572-579.
15. Knaust, R.K. and Nordlund, P. 2001. Screening for soluble expression of recombinant proteins in a 96-well format. Anal. Biochem. 297: 79-85.
16. Lee, S.R., Wong, J.M., and Collins, K. 2003. Human telomerase reverse transcriptase motifs required for elongation of a telomeric substrate. J. Biol. Chem. 278: 52531-52536.
17. Lingner, J., Hughes, T.R., Shevchenko, A., Mann, M., Lundblad, V., and Cech, T.R. 1997. Reverse transcriptase motifs in the catalytic subunit of telomerase. Science 276: 561-567.
18. Masutomi, K., Kaneko, S., Hayashi, N., Yamashita, T., Shirota, Y., Kobayashi, K., and Murakami, S. 2000. Telomerase activity reconstituted in vitro with purified human telomerase reverse transcriptase and human telomerase RNA component. J. Biol. Chem. 275: 22568-22573.
19. Mikuni, O., Trager, J.B., Ackerly,H.,Weinrich, S.L., Asai, A., Yamashita, Y., Mizukami, T., and Anazawa, H. 2002. Reconstitution of telomerase activity utilizing human catalytic subunit expressed in insect cells. Biochem. Biophys. Res. Commun. 298: 144-150.
20. Moriarty, T.J., Marie-Egyptienne, D.T., and Autexier, C. 2004. Functional organization of repeat addition processivity andDNA synthesis determinants in the human telomerase multimer. Mol. Cell. Biol. 24: 3720-3733.
21. O'Connor, C.M., Lai, C.K., and Collins, K. 2005. Two purified domains of telomerase reverse transcriptase reconstitute sequence-specific interactions with RNA. J. Biol. Chem. 17: 17533-17539.
22. Schnapp, G., Rodi, H.P., Rettig, W.J., Schnapp, A., and Damm, K. 1998. One-step affinity purification protocol for human telomerase. Nucleic Acids Res. 26: 3311-3313.
23. Sousa, R. 1996. Structural and mechanistic relationships between nucleic acid polymerases. Trends Biochem. Sci. 21: 186-190.
24. Waldo, G.S., Standish, B.M., Berendzen, J., and Terwilliger, T.C. 1999. Rapid protein-folding assay using green fluorescent protein. Nat. Biotechnol. 17: 691-695.
25. Weinrich, S.L., Pruzan, R., Ma, L., Ouellette, M., Tesmer, V.M., Holt, S.E., Bodnar, A.G., Lichtsteiner, S., Kim, N.W., Trager, J.B., et al. 1997. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT. Nat. Genet. 17: 498-502.
26. Xia, J., Peng, Y., Mian, I.S., and Lue, N.F. 2000. Identification of functionally important domains in the N-terminal region of telomerase reverse transcriptase. Mol. Cell. Biol. 20: 5196-5207.