Photochemical degradation of citrate buffers leads to covalent acetonation of recombinant protein therapeutics

Protein Science

Volumn 19, Issue 11, 2010, Pages 2152-2163

  Valliere Douglass, John F ^a   Connell Crowley, Lisa ^a   Jensen, Randy ^b   Schnier, Paul D ^b   Trilisky, Egor ^a   Leith, Matt ^a   Follstad, Brian D ^a   Kerr, Jennifer ^a   Lewis, Nathan ^a   Vunnum, Suresh ^a   Treuheit, Michael J ^a   Balland, Alain ^a   Wallace, Alison ^a  

^a AMGEN INC   (United States)

^b AMGEN INC   (United States)

Author keywords

Acetonation; Carbonylation; Citrate decarboxylation; Iron; Photo fenton; Protein acetone adducts; Protein aldimine adducts; Transition metal

Indexed keywords

AMINO ACID; BUFFER; CARBON 13; CARBONYL DERIVATIVE; CITRIC ACID; GLYCINE; IMINE; IRON; METHYL GROUP; RECOMBINANT PROTEIN; TRACE METAL;

ACETONATION; AMINO TERMINAL SEQUENCE; ARTICLE; CARBONYLATION; CHEMICAL REACTION; COVALENT BOND; DECOMPOSITION; DEGRADATION; LIGHT; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE; PHOTOCHEMISTRY; PRIORITY JOURNAL; PROTEIN MODIFICATION;

ACETONE; ANIMALS; ANTIBODIES, MONOCLONAL; CARBON ISOTOPES; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CITRATES; CRICETINAE; CRICETULUS; GLYCINE; HUMANS; IMINES; IMMUNOGLOBULIN G; IRON; ISOTOPE LABELING; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHOTOCHEMICAL PROCESSES; PROTEIN CARBONYLATION; RECOMBINANT PROTEINS;

EID: 78349246709     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.495     Document Type: Article

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