Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration

Journal of Virological Methods

Volumn 170, Issue 1-2, 2010, Pages 42-46

  Fumian, Tulio M ^a   Leite, José Paulo G ^a   Castello, Alejandro A ^b   Gaggero, Aldo ^c   Caillou, Maria Susana L de ^d   Miagostovich, Marize P ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b UNIVERSIDAD NACIONAL DE QUILMES   (Argentina)

^c UNIVERSITY OF CHILE   (Chile)

^d UNIVERSIDAD NACIONAL DE TUCUMÁN   (Argentina)

Author keywords

Adsorption elution method; Multiplex qPCR; PP7; Rotavirus A; Ultracentrifugation

Indexed keywords

ADSORPTION KINETICS; ARTICLE; BACTERIOPHAGE; BACTERIOPHAGE PP7; CONTROLLED STUDY; ELUTION; INTERMETHOD COMPARISON; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; ROTAVIRUS; ROTAVIRUS A; SEWAGE; ULTRACENTRIFUGATION; ULTRAFILTRATION; VIRUS CONCENTRATION; VIRUS DETECTION; VIRUS PURIFICATION; WASTE WATER;

ADSORPTION; GASTROENTERITIS; HUMANS; LEVIVIRUS; POLYMERASE CHAIN REACTION; RNA, VIRAL; ROTAVIRUS; SEWAGE; ULTRACENTRIFUGATION; ULTRAFILTRATION; WATER MICROBIOLOGY;

MONOPLEX; ROTAVIRUS A;

EID: 78249273634     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.08.017     Document Type: Article

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