One-step quantitative RT-PCR for the detection of rotavirus in acute gastroenteritis

Journal of Virological Methods

Volumn 153, Issue 2, 2008, Pages 238-240

  Zeng, S Q ^a   Halkosalo, A ^a   Salminen, M ^a   Szakal, E D ^a   Puustinen, L ^a   Vesikari, T ^a,b  

^a TAMPERE UNIVERSITY OF TECHNOLOGY   (Finland)

^b TAMPERE UNIVERSITY HOSPITAL   (Finland)

Author keywords

Gastroenteritis; Quantitative RT PCR; Rotavirus

Indexed keywords

ACUTE GASTROENTERITIS; ARTICLE; CROSS REACTION; DISEASE SEVERITY; ENZYME IMMUNOASSAY; FOLLOW UP; HUMAN; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ROTAVIRUS; SENSITIVITY ANALYSIS; VIRUS DETECTION; VIRUS INFECTION;

ACUTE DISEASE; CHILD, PRESCHOOL; DNA PRIMERS; FECES; GASTROENTERITIS; HUMANS; INFANT; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; ROTAVIRUS; ROTAVIRUS INFECTIONS; SENSITIVITY AND SPECIFICITY; TIME FACTORS; VIRAL NONSTRUCTURAL PROTEINS;

ROTAVIRUS;

EID: 53049102880     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.08.004     Document Type: Article

References (15)

1. Beards G.M., Campbell A.D., Cottrell N.R., Peiris J.S., Rees N., Sanders R.C., Shirley J.A., Wood H.C., and Flewett T.H. Enzyme-linked immunosorbent assays based on polyclonal and monoclonal antibodies for rotavirus detection. J. Clin. Microbiol. 19 (1984) 248-254
2. Boom R., Sol C.J.A., Salimans M.M.M., Jansen C.L., Wertheim-van Dillen P.M.E., and van der Noordaa J. Rapid and simple method for purifications of nucleic acids. J. Clin. Microbiol. 28 (1991) 495-503
3. Das B.K., Gentsch J.R., Cicirello H.G., Woods P.A., Gupta A., Ramachandran M., Kumar R., Bhan M.K., and Glass R.I. Characterization of rotavirus strains from newborns in New Delhi. India J. Clin. Microbiol. 32 (1994) 1820-1822
4. Gouvea V., Glass G.V., Woods R.I.P., Taniguchi K., Clark H.F., Forrester B., and Fang Z.Y. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J. Clin. Microbiol. 28 (1990) 276-282
5. Gunson R.N., Miller J., Leonard A., and Carman W.F. Importance of PCR in the diagnosis and understanding of rotavirus illness in the community. Commun. Dis. Public Health 6 (2003) 63-65
6. Kang G., Iturriza-Gomara M., Wheeler J.G., Crystal P., Monica B., Ramani S., Primrose B., Moses P.D., Gallimore C.I., Brown D.W., and Gray J. Quantitation of group A rotavirus by real-time reverse-transcription-polymerase chain reaction: correlation with clinical severity in children in South India. J. Med. Virol. 73 (2004) 118-122
7. Kutyavin I.V., Afonina I.A., Mills A., Gorn V.V., Lukhtanov E.A., and Belousov E.S. 3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures. Nucleic Acids Res. 28 (2000) 655-661
8. Mackay I.M., Arden K.E., and Nitsche A. Real-time PCR in virology. Nucleic Acids Res. 30 (2002) 1292-1305
9. Pang X.L., Joensuu J., Hoshino Y., Kapikian A.Z., and Vesikari T. Rotaviruses detected by reverse transcription polymerase chain reaction in acute gastroenteritis during a trial of rhesus-human reassortant rotavirus tetravalent vaccine, implication for vaccine efficacy analysis. J. Clin. Virol. 13 (1999) 9-16
10. Pang X.L., Lee B., Boroumand N., Leblanc B., Preiksaitis J.K., and Yu I.C.C. Increased detection of rotavirus using a real time reverse transcription-polymerase chain reaction (RT-PCR) assay in stool specimens from children with diarrhea. J. Med. Virol. 72 (2004) 496-501
11. Richardson S., Grimwood K., Gorrell R., Palombo E., Barnes G., and Bishop R. Extended excretion of rotavirus after severe diarrhoea in young children. Lancet 351 (1998) 1844-1848
12. Ruuska T., and Vesikari T. Rotavirus disease in Finnish children: use of numerical scores for clinical severity of diarrhoeal episodes. Scand. J. Infect. Dis. 22 (1990) 259-267
13. Vesikari T., Karvonen A., Puustinen L., Zeng S.Q., Szakal E.D., Delem A., and De Vos B. Efficacy of RIX4414 live attenuated human rotavirus vaccine in Finnish infants. Pediatr. Infect. Dis. J. 23 (2004) 937-943
14. Wilde J., Yolken R., Willoughby R., and Eiden J. Improved detection of rotavirus shedding by polymerase chain reaction. Lancet 337 (1991) 323-326
15. Yao Y., Nellåker C., and Karlsson H. Evaluation of minor groove binding probe and Taqman probe PCR assays: Influence of mismatches and template complexity on quantification. Mol. Cell Probes 20 (2006) 311-316