Development of a virus concentration method and its application to detection of enterovirus and Norwalk virus from coastal seawater

Applied and Environmental Microbiology

Volumn 68, Issue 3, 2002, Pages 1033-1039

  Katayama, Hiroyuki ^a   Shimasaki, Akihiro ^a   Ohgaki, Shinichiro ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GENES; MEMBRANES; PH EFFECTS; POSITIVE IONS; SEAWATER;

RECOVERY YIELDS;

VIRUSES;

SEA WATER;

DETECTION METHOD; SEAWATER; VIRUS;

ARTICLE; ELUTION; ENTEROVIRUS; JAPAN; NONHUMAN; NORWALK GASTROENTERITIS VIRUS; POLIOMYELITIS VIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEA POLLUTION; SEASHORE; TECHNIQUE; VIRUS ADSORPTION; VIRUS CONCENTRATION; VIRUS DETECTION;

ENTEROVIRUS; NORWALK VIRUS; SEAWATER; VIROLOGY; WATER MICROBIOLOGY;

JAPAN;

ENTEROVIRUS; NORWALK VIRUS; POLIOVIRUS; RNA VIRUSES;

EID: 0036196006     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.3.1033-1039.2002     Document Type: Article

References (45)

1. Detection of enteroviruses in groundwater with the PCR
2. A strategy for detection of viruses in groundwater by PCR
3. Detection of Norwalk virus and hepatitis A virus in shellfish tissues with the PCR
4. Detection of human enteric viruses in oysters by in vivo and in vitro amplification of nucleic acid
5. Applied and theoretical aspects of virus adsorption to surfaces
6. Practical direct plaque assay for coliphage in 100-ml samples of drinking water
7. A nested reverse transcriptase PCR assay for detection of small round-structured viruses in environmentally contaminated molluscan shellfish
8. Detection of viral pathogens by reverse transcriptase PCR and of microbial indicators by standard methods in the canals of the Florida Keys
9. Three-year study to assess human enteric viruses in shellfish
10. F-specific RNA bacteriophages in sewage: Methodology and occurrence
11. Bacteriophages as model viruses in water quality control
12. A method to remove environmental inhibitors prior to the detection of waterborne enteric viruses by reverse transcriptase-PCR
13. A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization
14. Detection of hepatitis E virus in raw and treated wastewater with the PCR
15. Immunomagnetic capture PCR for rapid concentration and detection of hepatitis A virus from environmental samples
16. Small, round-structured viruses (SRSVs) associated with acute gastroenteritis outbreak in Gifu, Japan
17. Detection of naturally occurring enteroviruses in water by reverse transcription, PCR, and hybridization
18. Fate of liberated viral RNA in waste-water determined by PCR
19. Application of PCR to detect coliphage Qβ in environmental water sample
20. Rapid concentration of bacteriophages from large volumes of freshwater: Evaluation of positively charged, microporous filters
21. A portable device for the rapid concentration of viruses from large volumes of natural freshwater
22. Influence of salts on virus adsorption to microporous filters
23. The recovery of viruses from drinking water by means of an in-line electropositive cartridge filter
24. Simple methodological approach for counting and identifying culturable viruses adsorbed to cellulose nitrate membrane filter
25. Detection and health risk associated with low virus concentration in drinking water
26. Detection of adenoviruses and enteroviruses in polluted water by nested PCR amplification
27. Comparison of methods for the recovery and quantification of coliphage and indigenous bacteriophage from marine water and sediments
28. Comparison of PCR and plaque assay for detection and enumeration of coliphage in polluted marine waters
29. Development of PCR methods for enteric virus detection in water
30. Concentration and purification of beef extract mock eluates from water samples for detection of enteroviruses, hepatitis A viruses, and Norwalk viruses by reverse transcription-PCR
31. Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR
32. Method to remove inhibitors in sewage and other fecal wastes for enterovirus detection by the PCR
33. Modified membrane-filter procedure for concentration of enteroviruses from tap water
34. Concentration of coliphage from water and sewage with charge-modified filter aid
35. Concentration of enteroviruses from large volumes of water
36. Concentration of poliovirus from tap water using positively charged microporous filters
37. Detection of hepatitis A virus in drinking water
38. Virus removal and recovery in the drinking water treatment train
39. Simple method of concentrating enteroviruses and hepatitis A virus from sewage and ocean water for rapid detection by reverse transcriptase PCR
40. Concentration of enteroviruses on membrane filters
41. A practical method for the concentration of viruses from water using fibriform cellulose and organic coagulant