Expression, purification, bioactivity, and partial characterization of a recombinant human bone morphogenetic protein-7 produced in human 293T cells

Molecular Biotechnology

Volumn 46, Issue 2, 2010, Pages 118-126

  Bustos Valenzuela, J C ^a   Halcsik, E ^a   Bassi, E J ^a   Demasi, M A ^a   Granjeiro, J M ^a,b   Sogayar, M C ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b FLUMINENSE FEDERAL UNIVERSITY   (Brazil)

Author keywords

Affinity chromatography purification; Lentiviral vector; Mammalian cell expression system; Recombinant human BMP 7

Indexed keywords

AFFINITY CHROMATOGRAPHY PURIFICATION; ALKALINE PHOSPHATASE ACTIVITY; BONE MORPHOGENETIC PROTEIN-7; C2C12 CELLS; CONDITIONED MEDIUM; CYTOKINES; ECTOPIC BONES; EXOGLYCOSIDASE; GROWTH FACTOR; HOMODIMERS; HUMAN BONE MORPHOGENETIC PROTEIN; IN-VITRO; IN-VIVO; KIDNEY DISEASE; LENTIVIRAL VECTOR; MAMMALIAN CELLS; MATURE PROTEINS; MESENCHYMAL CELLS; NUDE MICE; OSTEOINDUCTIVE PROPERTIES; PARTIAL CHARACTERIZATION; PURIFIED PROTEIN; RECOMBINANT HUMAN BMP-7; RECOMBINANT PROTEIN; T CELLS; WESTERN BLOTTING;

AFFINITY CHROMATOGRAPHY; BIOACTIVITY; BONE; MAMMALS; MEDICAL APPLICATIONS; PHOSPHATASES; PURIFICATION;

PROTEINS;

ALKALINE PHOSPHATASE; DIMER; GLYCOSIDASE; GLYCOSYLATED PROTEIN; LENTIVIRUS VECTOR; PROTEIN ANTIBODY; RECOMBINANT OSTEOGENIC PROTEIN 1;

AMINO ACID SEQUENCE; ARTICLE; CELL CULTURE; CELL STRAIN 293T; CELL STRAIN C2C12; CONTROLLED STUDY; CULTURE MEDIUM; EMBRYO CELL; GENE AMPLIFICATION; HUMAN; HUMAN CELL; MOLECULAR CLONING; OSSIFICATION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; STABLE TRANSFECTION; VIRAL GENE DELIVERY SYSTEM; WESTERN BLOTTING;

ANIMALS; BIOLOGICAL ASSAY; BONE MORPHOGENETIC PROTEIN 7; CHROMATOGRAPHY, AFFINITY; GENETIC VECTORS; HEK293 CELLS; HUMANS; LENTIVIRUS; MICE; MICE, INBRED BALB C; PLASMIDS; RECOMBINANT PROTEINS;

MAMMALIA; MUS MUSCULUS;

EID: 78149466997     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9287-0     Document Type: Article

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