Secretion of functional anti-CD30-angiogenin immunotoxins into the supernatant of transfected 293T-cells

Protein Expression and Purification

Volumn 28, Issue 2, 2003, Pages 211-219

  Stöcker, Michael ^a   Tur, Mehmet K ^a   Sasse, Stefanie ^b   Krüßmann, Anne ^b   Barth, Stefan ^a   Engert, Andreas ^b  

^a FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

^b UNIVERSITY HOSPITAL OF COLOGNE   (Germany)

Author keywords

Angiogenin; Eucaryotic expression; Hodgkin's lymphoma; Immunotherapy; Ki4(scFv); Recombinant immunotoxin

Indexed keywords

BACTERIA (MICROORGANISMS); EUKARYOTA;

EID: 0038733970     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00709-X     Document Type: Article

References (39)

1. R.J. Kreitman, I. Pastan, Recombinant toxins, Adv. Pharmacol. 28 (1994) 193-219.
2. R.J. Kreitman, Immunotoxins in cancer therapy, Curr. Opin. Immunol. 11 (5) (1999) 570-578.
3. R.J. Kreitman, Immunotoxins, Expert. Opin. Pharmacother. 1 (6) (2000) 1117-1129.
4. R. Schnell, E. Vitetta, J. Schindler, P. Borchmann, S. Barth, V. Ghetie, K. Hell, S. Drillich, V. Diehl, A. Engert, Treatment of refractory Hodgkin's lymphoma patients with an anti-CD25 ricin A-chain immunotoxin, Leukemia 14 (1) (2000) 129-135.
5. U. Winkler, S. Barth, R. Schnell, V. Diehl, A. Engert, The emerging role of immunotoxins in leukemia and lymphoma, Ann. Oncol. 8 (Suppl. 1) (1997) 139-146.
6. S.M. Rybak, H.R. Hoogenboom, H.M. Meade, J.C. Raus, D. Schwartz, R.J. Youle, Humanization of immunotoxins, Proc. Natl. Acad. Sci. USA 89 (8) (1992) 3165-3169.
7. D. Newton, Y. Xue, K. Olson, J. Fett, S. Rybak, Angiogenin single-chain immunofusions: influence of peptide linkers and spacers between fusion protein domains, Biochemistry 35 (2) (1996) 545-553.
8. M. Zewe, S. Rybak, S. Dubel, J. Coy, M. Welschof, D. Newton, M. Little, Cloning and cytotoxicity of a human pancreatic RNase immunofusion, Immunotechnology 3 (2) (1997) 127-136.
9. J.M. Yoon, S.H. Han, O.B. Kown, S.H. Kim, M.H. Park, B.K. Kim, Cloning and cytotoxicity of fusion proteins of EGF and angiogenin, Life Sci. 64 (16) (1999) 1435-1445.
10. D.L. Newton, S.M. Rybak, Preparation and preclinical characterization of RNase-based immunofusion proteins, Methods Mol. Biol. 160 (2001) 387-406.
11. D. FitzGerald, I. Pastan, J. Robertus, Clinical applications of immunotoxins introduction, Curr. Top. Microbiol. Immunol. 234 (1998) 1-11.
12. J. Buchner, I. Pastan, U. Brinkmann, A method for increasing the yield of properly folded recombinant fusion proteins: single-chain immunotoxins from renaturation of bacterial inclusion bodies, Anal. Biochem. 205 (2) (1992) 263-270.
13. I. Benhar, I. Pastan, Cloning, expression and characterization of the Fv fragments of the anti-carbohydrate mAbs B1 and B5 as single-chain immunotoxins, Protein Eng. 7 (12) (1994) 1509-1515.
14. S. Barth, M. Huhn, B. Matthey, A. Klimka, E.A. Galinski, A. Engert, Compatible-solute-supported periplasmic expression of functional recombinant proteins under stress conditions, Appl. Environ. Microbiol. 66 (4) (2000) 1572-1579.
15. J. Thomas, F. Baneyx, Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing heat-shock proteins, J. Biol. Chem. 271 (19) (1996) 11141-11147.
16. G. Georgiou, P. Valax, Expression of correctly folded proteins in Escherichia coli, Curr. Opin. Biotechnol. 7 (2) (1996) 190-197.
17. J.G. Wall, A. Pluckthun, The hierarchy of mutations influencing the folding of antibody domains in Escherichia coli, Protein Eng. 12 (7) (1999) 605-611.
18. M.J. Coloma, A. Hastings, L.A. Wims, S.L. Morrison, Novel vectors for the expression of antibody molecules using variable regions generated by polymerase chain reaction, J. Immunol. Methods 152 (1) (1992) 89-104.
19. J.B. Domachowske, K.D. Dyer, A.G. Adams, T.L. Leto, H.F. Rosenberg, Eosinophil cationic protein/RNase 3 is another RNase A-family ribonuclease with direct antiviral activity, Nucleic Acids Res. 26 (14) (1998) 3358-3363.
20. R.A. Spooner, D.J. Allen, A.A. Epenetos, J.M. Lord, Expression of immunoglobulin heavy chain-ricin A chain fusions in mammalian cells, Mol. Immunol. 31 (2) (1994) 117-125.
21. Y.Y. Liu, I. Gordienko, A. Mathias, S. Ma, J. Thompson, J.H. Woo, D.M. Neville Jr., Expression of an anti-CD3 single-chain immunotoxin with a truncated diphtheria toxin in a mutant CHO cell line, Protein Expr. Purif. 19 (2) (2000) 304-311.
22. A. Yang, S. Chen, A new class of antigen-specific killer cells [see comments], Nat. Biotechnol. 15 (1) (1997) 46-51.
23. S. Chen, A. Yang, J. Chen, T. Kute, C. King, J. Collier, Y. Cong, C. Yao, X. Huang, Potent antitumour activity of a new class of tumour-specific killer cells, Nature 385 (6611) (1997) 78-80.
24. D.A. Vallera, N. Jin, J.M. Baldrica, A. Panoskaltsis-Mortari, S.Y. Chen, B.R. Blazar, Retroviral immunotoxin gene therapy of acute myelogenous leukemia in mice using cytotoxic T cells transduced with an interleukin 4/diphtheria toxin gene, Cancer Res. 60 (4) (2000) 976-984.
25. J. Sambrook, E. Fritsch, T. Maniatis, Molecular Cloning. A Laboratory Manual, second ed., Cold Spring Harbor Laboratory Press, New York, 1989.
26. U. Kapp, J. Wolf, C. von Kalle, S. Tawadros, A. Rottgen, A. Engert, C. Fonatsch, H. Stein, V. Diehl, Preliminary report: growth of Hodgkin's lymphoma derived cells in immune compromised mice, Ann. Oncol. 3 (Suppl 4) (1992) 21-23.
27. F.L. Graham, J. Smiley, W.C. Russell, R. Nairn, Characteristics of a human cell line transformed by DNA from human adenovirus type 5, J. Gen. Virol. 36 (1) (1977) 59-74.
28. J. Bukovsky, A. Evans, M. Tartaglione, R.H. Kennett, Selection of variant neuroblastoma cell line which has lost cell surface expression of antigen detected by monoclonal antibody PI153/3, Somat. Cell Mol. Genet. 11 (5) (1985) 517-522.
29. B. Matthey, A. Engert, A. Klimka, V. Diehl, S. Barth, A new series of pET-derived vectors for high efficiency expression of Pseudomonas exotoxin-based fusion proteins, Gene 229 (1-2) (1999) 145-153.
30. A. Klimka, S. Barth, B. Matthey, R.C. Roovers, H. Lemke, H. Hansen, J.W. Arends, V. Diehl, H.R. Hoogenboom, A. Engert, An anti-CD30 single-chain Fv selected by phage display and fused to Pseudomonas exotoxin A (Ki-4(scFv)-ETA') is a potent immunotoxin against a Hodgkin-derived cell line, Br. J. Cancer 80 (8) (1999) 1214-1222.
31. J. Porath, J. Carlsson, I. Olsson, G. Belfrage, Metal chelate affinity chromatography, a new approach to protein fractionation, Nature 258 (5536) (1975) 598-599.
32. V. Hochuli, Cholesterol testing for all [letter], BMJ 298 (6677) (1989) 891.
33. S. Barth, M. Huhn, W. Wels, V. Diehl, A. Engert, Construction and in vitro evaluation of RFT5(scFv)-ETA', a new recombinant single-chain immunotoxin with specific cytotoxicity toward CD25+ Hodgkin-derived cell lines, Int. J. Mol. Med. 1 (1) (1998) 249-256.
34. S.M. Rybak, D.L. Newton, Natural and engineered cytotoxic ribonucleases: therapeutic potential, Exp. Cell Res. 253 (2) (1999) 325-335.
35. P.A. Leland, L.W. Schultz, B.M. Kim, R.T. Raines, Ribonuclease A variants with potent cytotoxic activity, Proc. Natl. Acad. Sci. USA 95 (18) (1998) 10407-10412.
36. M. Huhn, S. Sasse, M.K. Tur, B. Matthey, T. Schinkothe, S.M. Rybak, S. Barth, A. Engert, Human angiogenin fused to human CD30 ligand (Ang-CD30L) exhibits specific cytotoxicity against CD30-positive lymphoma, Cancer Res. 61 (24) (2001) 8737-8742.
37. C.J. Springer, I. Niculescu-Duvaz, Approaches to gene-directed enzyme prodrug therapy (GDEPT), Adv. Exp. Med. Biol. 465 (2000) 403-409.
38. M.E. Kadin, Pathology of Hodgkin's disease, Curr. Opin. Oncol. 6 (5) (1994) 456-463.
39. S. Chen, W. Marasco, Novel genetic immunotoxins and intracellular antibodies for cancer therapy, Semin. Oncol. 23 (1) (1996) 148-153.